Question about the accumulate time of the 5D spectrum?
To resolve overlapping resonances in a 2D spectrum of a protein acquired in 24 hours at 500MHz, a spectroscopist decided to measure a 5D spectrum using a 600MHz spectrometer. how long does he need to accumulate the 5D spectrum in order to reach the same signal-to-noise ratio as in the original 2D spectrum?
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it may take years to acquire a good resolution 5D spectrum using conventional sampling. Moreover a full 5D spectrum is a huge file (usually in T bytes) and its hard to navigate through such file for analysis. However..you can use time efficient acquisition by employing non-uniform sampling (NUS), also named as random or non-linear sampling. NUS data acquisition omits significant number of sampling points during the experiment and reconstruct them later. Because these methods require data sampled at uniform intervals, a two- to three-fold reduction in acquisition time can routinely be realized for each indirect time dimension that employs non uniform sampling.
For analysis..following may help. TSAR: a program for automatic resonance assignment using 2D cross-sections of high dimensionality, high-resolution spectra - Springer |
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