Nmrd
Hello!
Can someone explain to me how NMRD (nuclear magnetic relaxation dispersion) works? I found many papers where this technique was used but I could not find any explanations / pulse sequences for it. Essentially all I know is that you get a graph (vCPMG versus the transverse relaxation rate), from which you can extract information about time dynamic behaviour. So anything you can tell me is very welcome. Also I don't know what the vCPMG frequency is (CPMG = Carr-Purcell-Meiboom-Glll). best regards Vladimir |
Relaxation Dispersion
A good early paper that goes into some detail about the parameters and theory is:
Measurement of Slow (s-ms) Time Scale Dynamics in Protein Side Chains by 15N Relaxation Dispersion NMR Spectroscopy: Application to Asn and Gln Residues in a Cavity Mutant of T4 Lysozyme Frans A. A. Mulder, Nikolai R. Skrynnikov, Bin Hon, Frederick W. Dahlquist, and Lewis E. Kay* J. Am. Chem. Soc., 123 (5), 967 -975, 2001. 10.1021/ja003447g S0002-7863(00)03447-8 Web Release Date: January 16, 2001 although it is aimed at only at sidechain amide groups. A more comprehensive review can be found at: A.G. Palmer, C.D. Kroenke and J.P. Loria, NMR methods for quantifying microsecond-to-millisecond motions in biological macromolecules, Methods Enzymol. 339 (2001), pp. 204–238. Hope this helps |
All times are GMT. The time now is 07:15 AM. |
Powered by vBulletin® Version 3.7.3
Copyright ©2000 - 2024, Jelsoft Enterprises Ltd.
Search Engine Friendly URLs by vBSEO 3.6.0
Copyright, BioNMR.com, 2003-2013