BioNMR
NMR aggregator & online community since 2003
BioNMR    
Learn or help to learn NMR - get free NMR books!
 

Go Back   BioNMR > Educational resources > NMR Questions and Answers
Advanced Search



Jobs Groups Conferences Literature Pulse sequences Software forums Programs Sample preps Web resources BioNMR issues


Webservers
NMR processing:
MDD
NMR assignment:
Backbone:
Autoassign
MARS
UNIO Match
PINE
Side-chains:
UNIO ATNOS-Ascan
NOEs:
UNIO ATNOS-Candid
UNIO Candid
ASDP
Structure from NMR restraints:
Ab initio:
GeNMR
Cyana
XPLOR-NIH
ASDP
UNIO ATNOS-Candid
UNIO Candid
Fragment-based:
BMRB CS-Rosetta
Rosetta-NMR (Robetta)
Template-based:
GeNMR
I-TASSER
Refinement:
Amber
Structure from chemical shifts:
Fragment-based:
WeNMR CS-Rosetta
BMRB CS-Rosetta
Homology-based:
CS23D
Simshift
Torsion angles from chemical shifts:
Preditor
TALOS
Promega- Proline
Secondary structure from chemical shifts:
CSI (via RCI server)
TALOS
MICS caps, β-turns
d2D
PECAN
Flexibility from chemical shifts:
RCI
Interactions from chemical shifts:
HADDOCK
Chemical shifts re-referencing:
Shiftcor
UNIO Shiftinspector
LACS
CheckShift
RefDB
NMR model quality:
NOEs, other restraints:
PROSESS
PSVS
RPF scores
iCing
Chemical shifts:
PROSESS
CheShift2
Vasco
iCing
RDCs:
DC
Anisofit
Pseudocontact shifts:
Anisofit
Protein geomtery:
Resolution-by-Proxy
PROSESS
What-If
iCing
PSVS
MolProbity
SAVES2 or SAVES4
Vadar
Prosa
ProQ
MetaMQAPII
PSQS
Eval123D
STAN
Ramachandran Plot
Rampage
ERRAT
Verify_3D
Harmony
Quality Control Check
NMR spectrum prediction:
FANDAS
MestReS
V-NMR
Flexibility from structure:
Backbone S2
Methyl S2
B-factor
Molecular dynamics:
Gromacs
Amber
Antechamber
Chemical shifts prediction:
From structure:
Shiftx2
Sparta+
Camshift
CH3shift- Methyl
ArShift- Aromatic
ShiftS
Proshift
PPM
CheShift-2- Cα
From sequence:
Shifty
Camcoil
Poulsen_rc_CS
Disordered proteins:
MAXOCC
Format conversion & validation:
CCPN
From NMR-STAR 3.1
Validate NMR-STAR 3.1
NMR sample preparation:
Protein disorder:
DisMeta
Protein solubility:
camLILA
ccSOL
Camfold
camGroEL
Zyggregator
Isotope labeling:
UPLABEL
Solid-state NMR:
sedNMR


Reply
Thread Tools Search this Thread Rate Thread Display Modes
  #1  
Unread 12-24-2002, 12:08 PM
Junior Member
 
Join Date: Dec 2002
Posts: 1
Points: 12, Level: 1
Points: 12, Level: 1 Points: 12, Level: 1 Points: 12, Level: 1
Level up: 23%, 38 Points needed
Level up: 23% Level up: 23% Level up: 23%
Activity: 0%
Activity: 0% Activity: 0% Activity: 0%
NMR Credits: 0
NMR Points: 12
Downloads: 0
Uploads: 0
Default Answered: How does cryogenic probes inprove NMR experiments?

How does cryogenic probes inprove NMR experiments?
Reply With Quote


Did you find this post helpful? Yes | No
Best Answer - Posted by scientific_boy3434
lowering the temperature of the sample under the coalescence temperature (depends on the molecule) will give you more data, for example resolving temperature dependend J like N-H protons on amides.

  #2  
Unread 12-24-2002, 12:08 PM
Junior Member
 
Join Date: Dec 2002
Posts: 1
Points: 2, Level: 1
Points: 2, Level: 1 Points: 2, Level: 1 Points: 2, Level: 1
Level up: 3%, 48 Points needed
Level up: 3% Level up: 3% Level up: 3%
Activity: 0%
Activity: 0% Activity: 0% Activity: 0%
NMR Credits: 0
NMR Points: 2
Downloads: 0
Uploads: 0
Provided Answers: 1
Default How does cryogenic probes inprove NMR experiments?

lowering the temperature of the sample under the coalescence temperature (depends on the molecule) will give you more data, for example resolving temperature dependend J like N-H protons on amides.
Reply With Quote


1 out of 1 members found this post helpful. Did you find this post helpful? Yes | No
  #3  
Unread 12-25-2002, 01:34 AM
Junior Member
 
Join Date: Dec 2002
Posts: 1
Points: 2, Level: 1
Points: 2, Level: 1 Points: 2, Level: 1 Points: 2, Level: 1
Level up: 3%, 48 Points needed
Level up: 3% Level up: 3% Level up: 3%
Activity: 0%
Activity: 0% Activity: 0% Activity: 0%
NMR Credits: 0
NMR Points: 2
Downloads: 0
Uploads: 0
Default How does cryogenic probes inprove NMR experiments?

http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WJX-4BT1RP2-1&_user=10&_coverDate=05%2F31%2F2004&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=df54bfca7fe17072df3ea41064fd36d0http://www.rsc.org/publishing/journals/OC/article.asp?doi=b110384a

Content provided by Yahoo Answers.
Reply With Quote


0 out of 1 members found this post helpful. Did you find this post helpful? Yes | No
Reply
Similar Threads
Thread Thread Starter Forum Replies Last Post
Cryogenic temperature effects and resolution upon slow cooling of protein preparations in solid state NMR
Cryogenic temperature effects and resolution upon slow cooling of protein preparations in solid state NMR Abstract X-ray crystallography using synchrotron radiation and the technique of dynamic nuclear polarization (DNP) in nuclear magnetic resonance (NMR) require samples to be kept at temperatures below 100 K. Protein dynamics are poorly understood below the freezing point of water and down to liquid nitrogen temperatures. Therefore, we investigate the α-spectrin SH3 domain by magic angle spinning (MAS) solid state NMR (ssNMR) at various temperatures while cooling slowly. Cooling down...
nmrlearner Journal club 0 08-13-2011 02:47 AM
Cryogenic temperature effects and resolution upon slow cooling of protein preparations in solid state NMR.
Cryogenic temperature effects and resolution upon slow cooling of protein preparations in solid state NMR. Cryogenic temperature effects and resolution upon slow cooling of protein preparations in solid state NMR. J Biomol NMR. 2011 Aug 9; Authors: Linden AH, Franks WT, Akbey U, Lange S, van Rossum BJ, Oschkinat H X-ray crystallography using synchrotron radiation and the technique of dynamic nuclear polarization (DNP) in nuclear magnetic resonance (NMR) require samples to be kept at temperatures below 100*K. Protein dynamics are poorly understood...
nmrlearner Journal club 0 08-10-2011 12:30 PM
Slight mistuning of a cryogenic probe significantly perturbs the water 1H precession frequency
Slight mistuning of a cryogenic probe significantly perturbs the water 1H precession frequency Abstract A shift of the water proton precession frequency is described that can introduce errors in chemical shifts derived using the water signal as the chemical shift reference. This shift, fs, arises as a consequence of radiation damping when the water proton and detector circuit resonance frequencies differ. Herein it is shown that experimental values of fs, measured as a function of detector circuit tuning offset for 500 and 900 MHz cryogenic probes, are in good agreement with theory. Of...
nmrlearner Journal club 0 01-09-2011 12:46 PM
[NMR paper] Methyl groups as probes for proteins and complexes in in-cell NMR experiments.
Methyl groups as probes for proteins and complexes in in-cell NMR experiments. Related Articles Methyl groups as probes for proteins and complexes in in-cell NMR experiments. J Am Chem Soc. 2004 Jun 9;126(22):7119-25 Authors: Serber Z, Straub W, Corsini L, Nomura AM, Shimba N, Craik CS, Ortiz de Montellano P, Dtsch V Studying protein components of large intracellular complexes by in-cell NMR has so far been impossible because the backbone resonances are unobservable due to their slow tumbling rates. We describe a methodology that overcomes...
nmrlearner Journal club 0 11-24-2010 09:51 PM
[Question from NMRWiki Q&A forum] Please suggest experiments that push the power limits of NMR probes
Please suggest experiments that push the power limits of NMR probes I'm trying to come up with reasonable tests of probe performance for all channels by simulating conditions at which arcing is most likely to occur. For those tests I usually read voltage of the incoming wave on the RF line using directional coupler with a 50dB attenuated pickup and oscilloscope after running an "experiment" pulsing at the power and duty cycle that simulates the condition to be tested. If the waveform looks nice and square - there is no arcing. What experiments should I look into? OK for 1H I'd pick...
nmrlearner News from other NMR forums 0 10-08-2010 02:35 AM
[NMR paper] Use of paramagnetic NMR probes for structural analysis in cytochrome c3 from Desulfov
Use of paramagnetic NMR probes for structural analysis in cytochrome c3 from Desulfovibrio vulgaris. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_FREE_120x27.gif Related Articles Use of paramagnetic NMR probes for structural analysis in cytochrome c3 from Desulfovibrio vulgaris. Eur J Biochem. 1997 Mar 15;244(3):721-34 Authors: Salgueiro CA, Turner DL, Xavier AV The dipolar field generated by each of the four haems in the tetrahaem ferricytochrome c3 from...
nmrlearner Journal club 0 08-22-2010 03:31 PM
[NMR paper] NMR identification of protein surfaces using paramagnetic probes.
NMR identification of protein surfaces using paramagnetic probes. Related Articles NMR identification of protein surfaces using paramagnetic probes. Biochemistry. 1990 Oct 30;29(43):10041-8 Authors: Petros AM, Mueller L, Kopple KD Paramagnetic agents produce line broadening and thus cancellation of anti phase cross-peak components in two-dimensional correlated nuclear magnetic resonance spectra. The specificity of this effect was examined to determine its utility for identifying surface residues of proteins. Ubiquitin and hen egg white...
nmrlearner Journal club 0 08-21-2010 11:04 PM
Nitrogen-detected CAN and CON experiments as alternative experiments for main chain N
Abstract Heteronuclear direct-detection experiments, which utilize the slower relaxation properties of low γ nuclei, such as 13C have recently been proposed for sequence-specific assignment and structural analyses of large, unstructured, and/or paramagnetic proteins. Here we present two novel 15N direct-detection experiments. The CAN experiment sequentially connects amide 15N resonances using 13Cα chemical shift matching, and the CON experiment connects the preceding 13C? nuclei. When starting from the same carbon polarization, the intensities of nitrogen signals detected in the CAN or...
nmrlearner Journal club 0 08-14-2010 04:19 AM


Thread Tools Search this Thread
Search this Thread:

Advanced Search
Display Modes Rate This Thread
Rate This Thread:

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is On
Trackbacks are Off
Pingbacks are Off
Refbacks are Off



BioNMR advertisements to pay for website hosting and domain registration. Nobody does it for us.



Powered by vBulletin® Version 3.7.3
Copyright ©2000 - 2017, Jelsoft Enterprises Ltd.
Copyright, BioNMR.com, 2003-2013
Search Engine Friendly URLs by vBSEO 3.6.0

All times are GMT. The time now is 10:13 PM.


Map