BioNMR
NMR aggregator & online community since 2003
BioNMR    
Learn or help to learn NMR - get free NMR books!
 

Go Back   BioNMR > Educational resources > NMR pulse sequences
Advanced Search




Jobs Groups Conferences Literature Pulse sequences Software Sample preps Web resources BioNMR issues


Reply
Thread Tools Search this Thread Rate Thread Display Modes
  #1  
Unread 03-16-2005, 06:39 AM
nmrlearner's Avatar
Senior Member
 
Join Date: Jan 2005
Posts: 9,410
Points: 2,232, Level: 28
Points: 2,232, Level: 28 Points: 2,232, Level: 28 Points: 2,232, Level: 28
Level up: 55%, 68 Points needed
Level up: 55% Level up: 55% Level up: 55%
Activity: 99.9%
Activity: 99.9% Activity: 99.9% Activity: 99.9%
Last Achievements
Award-Showcase
NMR Credits: 0
NMR Points: 2,232
Downloads: 0
Uploads: 0
Default

The following NMR pulse sequences for biological macromolecules are available on the website of the Danish Instrument Center as of 3/15/05:
  • Coupling Constants in Proteins
    - 2D S3CT for measurement of one bond coupling constants (J & RDC) - Varian, Bruker
  • TROSY experiments
    - clean TROSY, 15N-1H correlation, gradient version, water flip back, optional t2 decoupling - Varian, Bruker
    - constant-time S3CT TROSY, 13 C-1H correlation, gradient version - Varian, Bruker
    - (H)CCH TROSY, 3D TROSY-type HCCH correlation with diagonal peak suppression - Varian
    - TROSY-Type NOESY, 2D NOESY with amide diagonal peak suppression - Varian
    - TROSY-NOESY, 3D version with amide diagonal peak suppression - Varian, Bruker
    - HNCA, 3D sequential HNCA for Protein Backbone Assignment - Varian, Bruker
    - HNCACB, 3D sequential HNCACB for Protein Backbone Assignment - Varian
  • J Coupling Constants across Hydrogen Bonds
    - 3D S3(HN) TROSY hHNCO pulse sequence for measurement of 2hJ(HN,CO) and 3hJ(N,CO) - Varian
    - 3D C E.COSY TROSY hHNCO pulse sequence for measurement of 3hJ(HN,C) - Varian
Reply With Quote


Did you find this post helpful? Yes | No
Reply
Similar Threads
Thread Thread Starter Forum Replies Last Post
[NMR paper] Sequential HNCACB and CBCANH protein NMR pulse sequences.
Sequential HNCACB and CBCANH protein NMR pulse sequences. Related Articles Sequential HNCACB and CBCANH protein NMR pulse sequences. J Magn Reson. 2001 Aug;151(2):328-31 Authors: Meissner A, Sørensen OW The pulse sequences HNCACB and CBCANH correlating side chain C(beta) resonances with amide resonances in the protein backbone do not distinguish between inter- and intraresidue correlations. The new pulse sequences sequential HNCACB and sequential CBCANH make this distinction by suppressing coherence transfer between 13C(alpha) and 15N via the...
nmrlearner Journal club 0 11-19-2010 07:44 PM
[Question from NMRWiki Q&A forum] Aromatic sidechain assignment pulse sequences for Varian?
Aromatic sidechain assignment pulse sequences for Varian? Hi, someone asked a similar question before, but I'd like to know: Are there readily available varian pulse sequences that help assign aromatic side-chains? Maybe there is something like that in Biopack or elsewhere? Thanks a bunch! M.
nmrlearner News from other NMR forums 0 09-14-2010 03:44 AM
[Question from NMRWiki Q&A forum] Varian Biopack pulse sequences for intermolecular NOEs
Varian Biopack pulse sequences for intermolecular NOEs Hi All, I'm trying to set up some experiments to determine intermolecular NOEs in a peptide:protein complex. At the moment from looking at the Biopack library, I have found 4 pulse sequences which will do this and each provide different information (gnoesyNhsqc_CN, gnoesyNhsqc_NN, gnoesyChsqc_CC and gnoesyChsqc_NC). Would people recommend using a combination of all four of these experiments to determine the intermolecular NOEs for use in structure calculations? Check if somebody has answered this question on NMRWiki QA forum
nmrlearner News from other NMR forums 0 08-22-2010 02:30 AM
[Question from NMRWiki Q&A forum] Bruker Avance pulse sequences - where to find if not in TopSpin?
Bruker Avance pulse sequences - where to find if not in TopSpin? I used to use Varian Inova systems with BioPack all the time, and now I'm using a Bruker Avance-III system geared for Chemistry, not biology. It's driving me crazy that I can't find the pulse sequences I'm used to using! Right now, I'd like to use a NOESY with WET water suppression, and I can't find one in the default set that came with the software. Where do I go for relatively simple pulse sequences for Avance systems? I see Bruker pulse sequences for advanced experiments on Ad Bax's site, at NMRFAM, and NMRWiki, but...
nmrlearner News from other NMR forums 0 08-22-2010 02:30 AM
[Question from NMRWiki Q&A forum] How do you port pulse sequences to different field strength?
How do you port pulse sequences to different field strength? Hi, when you set up a pulse sequence at a different magnet field strength - starting with an existing set of parameters - what do you look at? Does length of shaped pulses always need to be changed? What pulse lenghts need to be adjusted? When it is necessary to re-build RF waveforms - for what kinds of pulses?
nmrlearner News from other NMR forums 0 08-22-2010 02:30 AM
[Question from NMRWiki Q&A forum] Are hardcoded values in bruker pulse sequences overridable?
Are hardcoded values in bruker pulse sequences overridable? For example when there is a statement like this in the pulse program file "d12=20u" can I change value of d12 from the command line? Thanks.
nmrlearner News from other NMR forums 0 08-22-2010 02:30 AM
[Question from NMRWiki Q&A forum] 2D pulse sequences for NMR of protein/peptide complexes?
2D pulse sequences for NMR of protein/peptide complexes? Hello, I am looking for some 15N/13C-filtered 2D NOESY and 2D TOCSY pulse sequences to run on a Varian 600. Does anyone have any ideas where I would get some tried and tested pulse sequence input files? Check if somebody has answered this question on NMRWiki QA forum
nmrlearner News from other NMR forums 0 08-22-2010 02:30 AM
BMRB library of Bruker pulse sequences
The following Bruker pulse sequences can be found on BMRB website. Shift correlation: Homonuclear Three-dimensional TOCSY-HSQC 3D experiment NOESY-HSQC 3D experiment Heteronuclear
nmrlearner NMR pulse sequences 0 03-26-2005 05:18 AM


Thread Tools Search this Thread
Search this Thread:

Advanced Search
Display Modes Rate This Thread
Rate This Thread:

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is On
Trackbacks are Off
Pingbacks are Off
Refbacks are Off


Powered by vBulletin® Version 3.7.3
Copyright ©2000 - 2012, Jelsoft Enterprises Ltd.
vBCredits II Deluxe v1.2.0 Copyright © 2010 DragonByte Technologies
Copyright, BioNMR.com, 2003-2010
Search Engine Friendly URLs by vBSEO 3.6.0

All times are GMT. The time now is 06:36 AM.