2D TOCSY Experiment

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2D TOCSY Experiment

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NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

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08-19-2010, 02:34 PM

nmrlearner

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2D TOCSY Experiment

2D TOCSY Experiment

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[NMRpipe Yahoo group] Re: N15 separated NOESY and TOCSY Re: N15 separated NOESY and TOCSY Thank you for your replies. The processing script of TOCSY (NH plane): $NMRTXT/ext.xz.com fid/test%03d.fid xz001.fid nmrPipe -in xz001.fid \ ... -out xzf.ft2 More...	NMRpipe Yahoo group news	News from other NMR forums	0	05-29-2011 11:00 AM
[NMRpipe Yahoo group] Re: N15 separated NOESY and TOCSY Re: N15 separated NOESY and TOCSY Hi Iris, Another question is what do you mean by the NH vs HH planes in regards to the 3D matrix (a single 3D peak can't be positive viewed along one axis and More...	NMRpipe Yahoo group news	News from other NMR forums	0	05-27-2011 10:40 AM
[NMRpipe Yahoo group] Re: N15 separated NOESY and TOCSY Re: N15 separated NOESY and TOCSY Hi Iris, It's difficult to diagnose the problem without seeing the processing script, but what phase correction was used in the 15N dimension? If the initial More...	NMRpipe Yahoo group news	News from other NMR forums	0	05-26-2011 10:34 PM
[NMRpipe Yahoo group] N15 separated NOESY and TOCSY N15 separated NOESY and TOCSY Hello, I did a N15 separated TOCSY experiment and used the following script: #!/bin/csh bruk2pipe -in ./ser \ -bad 0.0 -aswap -DMX -decim 2632 -dspfvs 20 More...	NMRpipe Yahoo group news	News from other NMR forums	0	05-26-2011 10:34 PM
HNCA-TOCSY-CANH experiments with alternate 13C-12C labeling: a set of 3D experiment w HNCA-TOCSY-CANH experiments with alternate 13C-12C labeling: a set of 3D experiment with unique supra-sequential information for mainchain resonance assignment Abstract Described here is a set of three-dimensional (3D) NMR experiments that rely on CACA-TOCSY magnetization transfer via the weak 3 \textJ\textCa\textCa coupling. These pulse sequences, which resemble recently described 13C detected CACA-TOCSY (Takeuchi et al. 2010) experiments, are recorded in 1H2O, and use 1H excitation and detection. These experiments require alternate 13C-12C labeling together with perdeuteration,...	nmrlearner	Journal club	0	11-27-2010 07:34 PM
[Nature network NMR forum] HCCH-TOCSY (0 replies) HCCH-TOCSY (0 replies) Hello everybody, I am a biologist who has jumped into NMR :( I hope this is an active forum so that I can improve myself and can help someday to others. I have a very stupid question!!! Do you know if I can see some HN peaks correlated with C-beta in HCCH-TOCSY? Cheers,	nmrlearner	News from other NMR forums	0	08-25-2010 02:58 AM
[U. of Ottawa NMR Facility Blog] Hsqc - tocsy HSQC - TOCSY There are many different NMR techniques used to probe molecular structure. Two very useful methods are the HSQC (or HMQC) sequence used to obtain heteronuclear coupling correlations and the TOCSY sequence used to look at extended homonuclear coupling correlations. These are among the most widely used pulse sequences by chemists to help elucidate the molecular structure of organic molecules. The sequences can also be combined into a single HSQC-TOCSY sequence which is simply an HSQC followed by a TOCSY spin lock. For the case of 1H and 13C in organic molecules, the HSQC-TOCSY...	nmrlearner	News from NMR blogs	0	08-21-2010 08:15 PM
CACA-TOCSY with alternate 13Câ??12C labeling: a 13CÎ± direct detection experiment for Abstract We present a 13C direct detection CACA-TOCSY experiment for samples with alternate 13Câ??12C labeling. It provides inter-residue correlations between 13CÎ± resonances of residue i and adjacent CÎ±s at positions i â?? 1 and i + 1. Furthermore, longer mixing times yield correlations to CÎ± nuclei separated by more than one residue. The experiment also provides CÎ±-to-sidechain correlations, some amino acid type identifications and estimates for Ï? dihedral angles. The power of the experiment derives from the alternate 13Câ??12C labeling with glycerol or glycerol, which allows...	nmrlearner	Journal club	0	08-14-2010 04:19 AM

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