what is best pulse sequence for c13 edit noesy on vnmrj ?
 

what is best pulse sequence for c13 edit noesy on vnmrj ?

Hi

What is best pulse sequence in bio pack on vnmrj for C-13 edit NOESY ? I am working with 12 kd size protein which can exist as homo dimers ( i.e total size of homo-dimer is 24 kd ). Most frequent problems we are encountering as follows !line broadening , baseline , phase correction problems ( huge signal at 2.8 ppm )in first increments and f2f3 planes huge strips errors !

Thanking you in advance !



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