BioNMR
NMR aggregator & online community since 2003
BioNMR    
Learn or help to learn NMR - get free NMR books!
 

Go Back   BioNMR > NMR community > News from other NMR forums
Advanced Search



Jobs Groups Conferences Literature Pulse sequences Software forums Programs Sample preps Web resources BioNMR issues


Webservers
NMR processing:
MDD
NMR assignment:
Backbone:
Autoassign
MARS
UNIO Match
PINE
Side-chains:
UNIO ATNOS-Ascan
NOEs:
UNIO ATNOS-Candid
UNIO Candid
ASDP
Structure from NMR restraints:
Ab initio:
GeNMR
Cyana
XPLOR-NIH
ASDP
UNIO ATNOS-Candid
UNIO Candid
Fragment-based:
BMRB CS-Rosetta
Rosetta-NMR (Robetta)
Template-based:
GeNMR
I-TASSER
Refinement:
Amber
Structure from chemical shifts:
Fragment-based:
WeNMR CS-Rosetta
BMRB CS-Rosetta
Homology-based:
CS23D
Simshift
Torsion angles from chemical shifts:
Preditor
TALOS
Promega- Proline
Secondary structure from chemical shifts:
CSI (via RCI server)
TALOS
MICS caps, β-turns
d2D
PECAN
Flexibility from chemical shifts:
RCI
Interactions from chemical shifts:
HADDOCK
Chemical shifts re-referencing:
Shiftcor
UNIO Shiftinspector
LACS
CheckShift
RefDB
NMR model quality:
NOEs, other restraints:
PROSESS
PSVS
RPF scores
iCing
Chemical shifts:
PROSESS
CheShift2
Vasco
iCing
RDCs:
DC
Anisofit
Pseudocontact shifts:
Anisofit
Protein geomtery:
Resolution-by-Proxy
PROSESS
What-If
iCing
PSVS
MolProbity
SAVES2 or SAVES4
Vadar
Prosa
ProQ
MetaMQAPII
PSQS
Eval123D
STAN
Ramachandran Plot
Rampage
ERRAT
Verify_3D
Harmony
Quality Control Check
NMR spectrum prediction:
FANDAS
MestReS
V-NMR
Flexibility from structure:
Backbone S2
Methyl S2
B-factor
Molecular dynamics:
Gromacs
Amber
Antechamber
Chemical shifts prediction:
From structure:
Shiftx2
Sparta+
Camshift
CH3shift- Methyl
ArShift- Aromatic
ShiftS
Proshift
PPM
CheShift-2- Cα
From sequence:
Shifty
Camcoil
Poulsen_rc_CS
Disordered proteins:
MAXOCC
Format conversion & validation:
CCPN
From NMR-STAR 3.1
Validate NMR-STAR 3.1
NMR sample preparation:
Protein disorder:
DisMeta
Protein solubility:
camLILA
ccSOL
Camfold
camGroEL
Zyggregator
Isotope labeling:
UPLABEL
Solid-state NMR:
sedNMR


Reply
Thread Tools Search this Thread Rate Thread Display Modes
  #1  
Unread 01-25-2013, 10:19 AM
nmrlearner's Avatar
Senior Member
 
Join Date: Jan 2005
Posts: 18,080
Points: 193,617, Level: 100
Points: 193,617, Level: 100 Points: 193,617, Level: 100 Points: 193,617, Level: 100
Level up: 0%, 0 Points needed
Level up: 0% Level up: 0% Level up: 0%
Activity: 50.7%
Activity: 50.7% Activity: 50.7% Activity: 50.7%
Last Achievements
Award-Showcase
NMR Credits: 0
NMR Points: 193,617
Downloads: 0
Uploads: 0
Default spinning problem on varian probe

spinning problem on varian probe

We are using a Varian300autoSW probe, which had previously worked well with no problems spinning samples. We had switched this probe out with a solids probe for some time and when we put the liquids probe back in, the liquids probe was no longer able to spin samples. We are well aware of all of the problems with dirt in the upper bore tube or on the spinner causing problems with sample spinning and have taken to time clean each of these throroughly. We believe that somehow there is a problem with the VNMRJ (2.2D) software setting that occured after switching probes. The reason we think this is that we have an old sundisk we previously used for this instrument. When I hook up the old sundisk (vnrmj 1.1D), the sample spins with no problems. Something we think might also be relevant is the CPU running VNMRJ 2.2D reports an error message in the terminal screen: 'masSpeedWrap.c' line: 139, * Speed Controller had problem with 'S' command.

This message is clearly about the solids probe. We've searched the vnmrj manuals and throughout the internet but cannot find a solution for our VNMRJ2.2D computer not being able to spin samples or what is causing the MAS error message.

If anyone has any suggestions on how to proceed, we would be extremely grateful

Thanks

Quinn Best



Check if somebody has answered this question on NMRWiki QA forum
Reply With Quote


Did you find this post helpful? Yes | No

Reply
Similar Threads
Thread Thread Starter Forum Replies Last Post
[Question from NMRWiki Q&A forum] Setting up deuterium (2H) NMR on Bruker BBO probe
Setting up deuterium (2H) NMR on Bruker BBO probe I need to set up 2H NMR on a Bruker 600 MHz BBO probe for the first time. Can anyone give me a set of instructions? Do I run in protonated or deuterated solvent? How do I tune 2H on the BB channel? What 2H values do I need in the edprosol table? What pulse program? What hardware connections or disconnections, e.g. removing the 2H stop filter.... Check if somebody has answered this question on NMRWiki QA forum
nmrlearner News from other NMR forums 0 09-17-2012 02:13 PM
[Question from NMRWiki Q&A forum] Calibration of a Bruker NMR probe
Calibration of a Bruker NMR probe I've received a loaner probe from Bruker while ours is being repaired but I'm not getting any timely response on instructions on calibrating it for use. Anyone have a resource or documentation? Thanks, Ken
nmrlearner News from other NMR forums 0 06-16-2012 06:01 AM
[Question from NMRWiki Q&A forum] spinner problem [Varian]
spinner problem Dear Colleagues, my NMR system recently suffers from problems with spinning the sample. Namely, it does not regulate at the set spinning rate at all in any given period of time. The spinning frequency changes continuously and never stabilizes. I wonder what may be the reason for that? Which part of the system should I inspect in the first place looking for the solution? Thank you in advance! All the best, D. Kubicki
nmrlearner News from other NMR forums 0 03-15-2012 06:10 AM
[U. of Ottawa NMR Facility Blog] Probe Tuning and 90 Degree Pulses
Probe Tuning and 90 Degree Pulses In order to get meaningful results from multiple-pulse NMR pulse sequences, it is essential that the 90 and 180 pulses are calibrated at the power levels used in the sequences (see this post for example). The calibrations are usually done on a standard sample in a well tuned and matched probe. The calibrations are typically stored in a file which is called up when setting up particular NMR experiments. It is important to know that these calibrations are correct for the particular sample of interest only when the probe is well tuned and matched. For...
nmrlearner News from NMR blogs 0 09-16-2011 10:02 PM
solution to a basic nmr problem, practice problem 3
solution to a basic nmr problem, practice problem 3 http://i.ytimg.com/vi/mOdABDblqKo/default.jpg solution to a basic nmr problem, practice problem 3 The solution to a good, basic practice nmr and IR problem at Bryn Mawr College by M Nerz From: mnerzsto Views: 4060 http://gdata.youtube.com/static/images/icn_star_full_11x11.gif http://gdata.youtube.com/static/images/icn_star_full_11x11.gif http://gdata.youtube.com/static/images/icn_star_full_11x11.gif http://gdata.youtube.com/static/images/icn_star_full_11x11.gif http://gdata.youtube.com/static/images/icn_star_full_11x11.gif ...
nmrlearner NMR educational videos 0 09-14-2011 09:34 PM
[Question from NMRWiki Q&A forum] Tuning probe failed after a dual probe was replaced with a BBI probe
Tuning probe failed after a dual probe was replaced with a BBI probe We generally use Dual to run 13C and BBI to run 2D. After changed the probe, the command "edhead" was used to set the probe. Put the sample tube, lock the solvent, and then type "atma" to tune the probe. We always do it like this, but now we can not tune the proton after installed the BBI probe (13C is OK). The dip can not be found by "atma", and "atmm" was also not work on forming a dip. What is the most possible reason for this error? How to solve it and avoid it in the future ? Thanks. (Instrument: Bruker 400 MHz,...
nmrlearner News from other NMR forums 0 08-23-2011 05:31 PM
[NMR paper] Using NMR as a probe of protein structure and function.
Using NMR as a probe of protein structure and function. Related Articles Using NMR as a probe of protein structure and function. Biochem Soc Trans. 1999 Aug;27(4):701-13 Authors: Malthouse JP
nmrlearner Journal club 0 11-18-2010 08:31 PM
HELP!!!!! Probe Needed
I need a 300 MHz long bore broadband 1H carbon probe. Does anyone have one laying around they dont need anymore??????
mblume83 NMR Questions and Answers 0 05-08-2007 08:04 PM


Thread Tools Search this Thread
Search this Thread:

Advanced Search
Display Modes Rate This Thread
Rate This Thread:

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is On
Trackbacks are Off
Pingbacks are Off
Refbacks are Off



BioNMR advertisements to pay for website hosting and domain registration. Nobody does it for us.



Powered by vBulletin® Version 3.7.3
Copyright ©2000 - 2017, Jelsoft Enterprises Ltd.
Copyright, BioNMR.com, 2003-2013
Search Engine Friendly URLs by vBSEO 3.6.0

All times are GMT. The time now is 09:08 AM.


Map