Question from NMRWiki Q&A forum Should I decouple deuterium on 2H/15N labeled sample for R1, R2 and hetnoe?

		BioNMR > NMR community > News from other NMR forums
[Question from NMRWiki Q&A forum] Should I decouple deuterium on 2H/15N labeled sample for R1, R2 and hetnoe?

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

View First Unread

Thread Tools

Search this Thread

Rate Thread

Display Modes

08-22-2010, 02:30 AM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,174

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Should I decouple deuterium on 2H/15N labeled sample for R1, R2 and hetnoe?

Should I decouple deuterium on 2H/15N labeled sample for R1, R2 and hetnoe?

If I'm running relaxation experiments (15N) for R1, R2, and hetnoe on a double labeled (2H/15N) sample, do I need to do any deuterium decoupling added to the program?

Check if somebody has answered this question on NMRWiki QA forum

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
[Question from NMRWiki Q&A forum] 2DNOESY with N15 C13 Labelled sample or Unlabelled sample 2DNOESY with N15 C13 Labelled sample or Unlabelled sample Dear Friends, I am in process of doing structure calculation of dimeric protein 22kD by using ARIA2.2 . Can I acquire 2DNOESY spectrum for my protein. Will it provide useful information for structure calculation? And whether I should acquire 2DNOESY with N15 C13 Labelled sample or Unlabelled sample . Which sample would be appropriate for this kind of experiment on VNMRJ 700 with biopack ? Thanks & Regards Srinivas Check if somebody has answered this question on NMRWiki QA forum	nmrlearner	News from other NMR forums	0	02-06-2012 03:54 PM
NMR sample.JPG http://upload.wikimedia.org/wikimedia/commons/thumb/b/b0/NMR_sample.JPG/225px-NMR_sample.JPG Uploaded by user "Armando-Martin" on Mon, 25 Oct 2010 01:05:00 UTC Added to category on Mon, 25 Oct 2010 01:05:51 UTC Original image: 1704×2272 pixel; 666.542 bytes. Licensing : Public domain NMR sample.JPG More...	nmrlearner	NMR pictures	0	11-01-2010 08:38 AM
[Question from NMRWiki Q&A forum] What has been done with direct detection of Deuterium? What has been done with direct detection of Deuterium? Hello, I am trying to investigate what has been done in NMR using direct detection of deuterium in liquids. - Could you suggest any citations? What kind of sensitivity gain there is in using broadband probe vs. lock channel? Has anything been done/can be done to detect scalar couplings with protons like in COSY or TOCSY? Are HD couplings other than geminal detectable? Thanks.	nmrlearner	News from other NMR forums	0	08-22-2010 02:30 AM
[NMRwiki tweet] nmrwiki: Should deuterium be decoupled in R2, R1 hetnoe expts for 2H/15N labeled samp nmrwiki: Should deuterium be decoupled in R2, R1 hetnoe expts for 2H/15N labeled sample? http://qa.nmrwiki.org/question/161/ nmrwiki: Should deuterium be decoupled in R2, R1 hetnoe expts for 2H/15N labeled sample? http://qa.nmrwiki.org/question/161/ Source: NMRWiki tweets	nmrlearner	Twitter NMR	0	08-22-2010 01:49 AM
[U. of Ottawa NMR Facility Blog] Deuterium Decoupling Deuterium Decoupling Bruker NMR spectrometers equipped with a 2H TX board and 2H amplifier can be setup to carry out 2H observation and decoupling experiments while still maintaining a 2H lock. Two channel systems can use the 2H transmitter for 2H, 1H, 2H and X measurements. In addition to these, a three channel system can be set up for X measurements. The first figure below shows the 13C and 13C spectra collected for a mixture of deuterated solvents using two channels of an AVANCE spectrometer. The second figure shows the 13C, 13C, 13C and 13C spectra for the standard 13C sensitivity...	nmrlearner	News from NMR blogs	0	08-21-2010 08:15 PM
[NMRpipe Yahoo group] Re: Deuterium referencing in conv.tcl Re: Deuterium referencing in conv.tcl Greetings all ... Alex is absolutely correct, and I get to wear the Deuterium Dunce Cap. We've updated and posted new versions of NMRPipe with this fix -- so, More...	nmrlearner	News from other NMR forums	0	08-18-2010 11:15 AM
[NMRpipe Yahoo group] Deuterium referencing in conv.tcl Deuterium referencing in conv.tcl Hello, This isn't of immediate relevance to me, but I am curious nonetheless. I was looking through conv.tcl to learn a bit of how the varian and bruker More...	nmrlearner	News from other NMR forums	0	08-18-2010 11:15 AM
Asking about Deuterium NMR I wanted to do Deuterium NMR in human urine sample but no one has done previously in our lab. In our Lab. we have 400 MHz Bruker (Avance) spectrometer with BroadBand Inverse probe (1H Observe, Z-gradient). How we have to proceed without deuterium lock which is neccessory for exp. ? Is above described instruments specification sufficient to perform the deutrium NMR experiments ? Please any on can help Thanks very much	santoshbharti	NMR Questions and Answers	3	01-14-2009 03:49 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off