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Default S/N increases then decreases in 2D HMBC-type experiment

S/N increases then decreases in 2D HMBC-type experiment

Hello All

Does anyone have any ideas on why the S/N would increase and then degrade during an HMBC-style experiment? If I process as I acquire, the S/N is best at about 3/4 through the experiment. At the end, the signals are still visible but the S/N has decreased. Both the instrument and the pulse sequence/pulse program appear to be working correctly (tested with other samples).

Thank-you in advance.



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