Randomly duterated protein is useful to get noesy experments ?
Randomly duterated protein is useful to get noesy experments ?
Dear NMR WIKIers I got doubt that can I use Randomly duterated protein (40 to 60 percent ) to get 3D (C13 EDIT and N15 EDIT) noesy experiments ? because if i duteriated the protein randomly the peak intensity drop would be there. but from standard formula NOE = 1/R6. I am afraid of error from intensity drop . could you please suggest any reference related to this ? Thnaking you Check if somebody has answered this question on NMRWiki QA forum |
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