Has someone tried to run proton decoupled of 14N? How long should be the 14N decoupling pulse? I had some problems with the lock signal (lost it) when I tried the pulse sequence zgig (1H on the observed channel). When recording 1D 14N spectrum, the lock was fine (it had some small distortions, but was not lost). We have a DRX 400 MHz spectrometer, with Topspin 1.3 and a BBFO atma probe.Any suggestions on how to set up this experiment will be highly appreciated. Thanks a lot, alina
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NMR Detection of pH-Dependent Histidine–Water Proton Exchange Reveals the Conduction Mechanism of a Transmembrane Proton Channel
NMR Detection of pH-Dependent Histidine–Water Proton Exchange Reveals the Conduction Mechanism of a Transmembrane Proton Channel
Fanghao Hu, Klaus Schmidt-Rohr and Mei Hong
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja2081185/aop/images/medium/ja-2011-081185_0008.gif
Journal of the American Chemical Society
DOI: 10.1021/ja2081185
http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA
http://feeds.feedburner.com/~r/acs/jacsat/~4/C3pPoB5_PR8
nmrlearner
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10-22-2011 10:16 AM
[NMR paper] Direct refinement against proton-proton dipolar couplings in NMR structure determinat
Direct refinement against proton-proton dipolar couplings in NMR structure determination of macromolecules.
Related Articles Direct refinement against proton-proton dipolar couplings in NMR structure determination of macromolecules.
J Magn Reson. 2000 Feb;142(2):393-6
Authors: Tjandra N, Marquardt J, Clore GM
The computational tools necessary for making use of (1)H-(1)H dipolar couplings in macromolecular structure refinement are presented. Potentials are described for direct refinement against (1)H-(1)H dipolar couplings of known sign as well...
nmrlearner
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11-18-2010 09:15 PM
Mechanisms of proton conduction and gating in influenza m2 proton channels from solid
Mechanisms of proton conduction and gating in influenza m2 proton channels from solid-state NMR.
Related Articles Mechanisms of proton conduction and gating in influenza m2 proton channels from solid-state NMR.
Science. 2010 Oct 22;330(6003):505-8
Authors: Hu F, Luo W, Hong M
The M2 protein of influenza viruses forms an acid-activated tetrameric proton channel. We used solid-state nuclear magnetic resonance spectroscopy to determine the structure and functional dynamics of the pH-sensing and proton-selective histidine-37 in M2 bound to a...
nmrlearner
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10-23-2010 05:48 PM
[NMRwiki tweet] nmrwiki: Should deuterium be decoupled in R2, R1 hetnoe expts for 2H/15N labeled samp
nmrwiki: Should deuterium be decoupled in R2, R1 hetnoe expts for 2H/15N labeled sample? http://qa.nmrwiki.org/question/161/
nmrwiki: Should deuterium be decoupled in R2, R1 hetnoe expts for 2H/15N labeled sample? http://qa.nmrwiki.org/question/161/
Source: NMRWiki tweets
nmrlearner
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08-22-2010 01:49 AM
Structure determination of proteins in 2H2O solution aided by a deuterium-decoupled 3
Abstract We developed an NMR pulse sequence, 3D HCA(N)CO, to correlate the chemical shifts of protein backbone 1Hα and 13Cα to those of 13C� in the preceding residue. By applying 2H decoupling, the experiment was accomplished with high sensitivity comparable to that of HCA(CO)N. When combined with HCACO, HCAN and HCA(CO)N, the HCA(N)CO sequence allows the sequential assignment using backbone 13C� and amide 15N chemical shifts without resort to backbone amide protons. This assignment strategy was demonstrated for 13C/15N-labeled GB1 dissolved in 2H2O. The quality of the GB1 structure...
proton decoupled of 14N
Linear Mode
