Protein-Protein Interactions
 

Protein-Protein Interactions

Hi i'm a newbie in NMR and I have 2 proteins, A protein is about 12KDa, B protein is about 45 KDa,i know that A protein binds ssRNA 20nucleotide molecule. My question what is the best way to know if protein B forms a complex A and RNA using NRM techniques? I have thought of deutering protein B, and mapping chemical shifts in isotopic labelled (15N) A protein, is this ok?... can transfered NOE or filtered NOE be used?? what about PRE? I've heard of TROSY, how can this help me?

Thanks for your ideas. Structural biology Master student



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