BioNMR
NMR aggregator & online community since 2003
BioNMR    
Learn or help to learn NMR - get free NMR books!
 

Go Back   BioNMR > NMR community > News from other NMR forums
Advanced Search



Jobs Groups Conferences Literature Pulse sequences Software forums Programs Sample preps Web resources BioNMR issues


Webservers
NMR processing:
MDD
NMR assignment:
Backbone:
Autoassign
MARS
UNIO Match
PINE
Side-chains:
UNIO ATNOS-Ascan
NOEs:
UNIO ATNOS-Candid
UNIO Candid
ASDP
Structure from NMR restraints:
Ab initio:
GeNMR
Cyana
XPLOR-NIH
ASDP
UNIO ATNOS-Candid
UNIO Candid
Fragment-based:
BMRB CS-Rosetta
Rosetta-NMR (Robetta)
Template-based:
GeNMR
I-TASSER
Refinement:
Amber
Structure from chemical shifts:
Fragment-based:
WeNMR CS-Rosetta
BMRB CS-Rosetta
Homology-based:
CS23D
Simshift
Torsion angles from chemical shifts:
Preditor
TALOS
Promega- Proline
Secondary structure from chemical shifts:
CSI (via RCI server)
TALOS
MICS caps, β-turns
d2D
PECAN
Flexibility from chemical shifts:
RCI
Interactions from chemical shifts:
HADDOCK
Chemical shifts re-referencing:
Shiftcor
UNIO Shiftinspector
LACS
CheckShift
RefDB
NMR model quality:
NOEs, other restraints:
PROSESS
PSVS
RPF scores
iCing
Chemical shifts:
PROSESS
CheShift2
Vasco
iCing
RDCs:
DC
Anisofit
Pseudocontact shifts:
Anisofit
Protein geomtery:
Resolution-by-Proxy
PROSESS
What-If
iCing
PSVS
MolProbity
SAVES2 or SAVES4
Vadar
Prosa
ProQ
MetaMQAPII
PSQS
Eval123D
STAN
Ramachandran Plot
Rampage
ERRAT
Verify_3D
Harmony
Quality Control Check
NMR spectrum prediction:
FANDAS
MestReS
V-NMR
Flexibility from structure:
Backbone S2
Methyl S2
B-factor
Molecular dynamics:
Gromacs
Amber
Antechamber
Chemical shifts prediction:
From structure:
Shiftx2
Sparta+
Camshift
CH3shift- Methyl
ArShift- Aromatic
ShiftS
Proshift
PPM
CheShift-2- Cα
From sequence:
Shifty
Camcoil
Poulsen_rc_CS
Disordered proteins:
MAXOCC
Format conversion & validation:
CCPN
From NMR-STAR 3.1
Validate NMR-STAR 3.1
NMR sample preparation:
Protein disorder:
DisMeta
Protein solubility:
camLILA
ccSOL
Camfold
camGroEL
Zyggregator
Isotope labeling:
UPLABEL
Solid-state NMR:
sedNMR


Reply
Thread Tools Search this Thread Rate Thread Display Modes
  #1  
Unread 10-30-2011, 01:39 PM
nmrlearner's Avatar
Senior Member
 
Join Date: Jan 2005
Posts: 23,135
Points: 193,617, Level: 100
Points: 193,617, Level: 100 Points: 193,617, Level: 100 Points: 193,617, Level: 100
Level up: 0%, 0 Points needed
Level up: 0% Level up: 0% Level up: 0%
Activity: 50.7%
Activity: 50.7% Activity: 50.7% Activity: 50.7%
Last Achievements
Award-Showcase
NMR Credits: 0
NMR Points: 193,617
Downloads: 0
Uploads: 0
Default Protein-peptide binding NMR Experments

Protein-peptide binding NMR Experments

Hello friends ,

Protein exist as multimeter (i.e Monomer,dimer,tetrameter ) Before binding to the peptide . After peptide binding to the protein ,we are assuming that protein exist as dimer .

So could you please suggest any supporting NMR experiments and reference to prove protein's Dimer state ?



Check if somebody has answered this question on NMRWiki QA forum
Reply With Quote


Did you find this post helpful? Yes | No

Reply
Similar Threads
Thread Thread Starter Forum Replies Last Post
[Question from NMRWiki Q&A forum] Randomly duterated protein is useful to get noesy experments ?
Randomly duterated protein is useful to get noesy experments ? Dear NMR WIKIers I got doubt that can I use Randomly duterated protein (40 to 60 percent ) to get 3D (C13 EDIT and N15 EDIT) noesy experiments ? because if i duteriated the protein randomly the peak intensity drop would be there. but from standard formula NOE = 1/R6. I am afraid of error from intensity drop . could you please suggest any reference related to this ? Thnaking you
nmrlearner News from other NMR forums 0 10-03-2011 08:39 AM
[Question from NMRWiki Q&A forum] Auto assignment of N15 C13 EDIT noesy experments
Auto assignment of N15 C13 EDIT noesy experments Dear NMR wikiers could you please suggest any auto assignment web servers online to assign N-15 & C13- EDIT NOESY experiments data? Thanking you
nmrlearner News from other NMR forums 0 09-29-2011 07:27 AM
[Question from NMRWiki Q&A forum] optimization of mixing time 3D NOESY experments
optimization of mixing time 3D NOESY experments Could you please explain that how to optimize mixing time for 3D NOESY experiments for protein like C13 edit NOESY and N15 EDIT NOESY and i want keep spin diffusion as much as less ? Check if somebody has answered this question on NMRWiki QA forum
nmrlearner News from other NMR forums 0 09-21-2011 05:25 AM
[Question from NMRWiki Q&A forum] Aminoacid residue identification or assignment of protein from 3D NMR Experments
Aminoacid residue identification or assignment of protein from 3D NMR Experments Dear NMR Wiki'ers I am identifying aminoacid residues or assignment of protein from following 3D NMR experments HNCA/HNCOCA - Glycine (45 ppm ) CBCANH/CBCACONH - Alanine (52-CA , 19 - CB ), Serine,threonine - ( CA-58.57 , CB - 64 or 70 )
nmrlearner News from other NMR forums 0 05-15-2011 08:19 PM
Transferred NOESY NMR studies of biotin mimetic peptide (FSHPQNT) bound to streptavidin: A structural model for studies of peptide-protein interactions.
Transferred NOESY NMR studies of biotin mimetic peptide (FSHPQNT) bound to streptavidin: A structural model for studies of peptide-protein interactions. Transferred NOESY NMR studies of biotin mimetic peptide (FSHPQNT) bound to streptavidin: A structural model for studies of peptide-protein interactions. Chem Biol Drug Des. 2011 Feb 5; Authors: Gizachew D, Dratz E Protein-protein interactions control signaling, specific adhesion and many other biological functions. The three dimensional structures of the interfaces and bound ligand can be...
nmrlearner Journal club 0 02-08-2011 06:28 PM
[NMR paper] NMR solution structure of a peptide from the mdm-2 binding domain of the p53 protein
NMR solution structure of a peptide from the mdm-2 binding domain of the p53 protein that is selectively cytotoxic to cancer cells. Related Articles NMR solution structure of a peptide from the mdm-2 binding domain of the p53 protein that is selectively cytotoxic to cancer cells. Biochemistry. 2004 Feb 24;43(7):1854-61 Authors: Rosal R, Pincus MR, Brandt-Rauf PW, Fine RL, Michl J, Wang H We have recently found that a peptide from the mdm-2 binding domain of the p53 protein induced rapid membranolytic necrosis of a variety of different human...
nmrlearner Journal club 0 11-24-2010 09:25 PM
[NMR paper] Peptide hormone binding to G-protein-coupled receptors: structural characterization v
Peptide hormone binding to G-protein-coupled receptors: structural characterization via NMR techniques. Related Articles Peptide hormone binding to G-protein-coupled receptors: structural characterization via NMR techniques. Med Res Rev. 2001 Sep;21(5):450-71 Authors: Mierke DF, Giragossian C G-protein-coupled receptors (GPCRs) allow cells to respond to calcium, hormones, and neurotransmitters. Not surprisingly, they currently make up the largest family of validated drug targets. Rational drug design for molecular regulators targeting GPCRs...
nmrlearner Journal club 0 11-19-2010 08:44 PM
Crystallographic and NMR evaluation of the impact of peptide binding to the second PD
Crystallographic and NMR evaluation of the impact of peptide binding to the second PDZ domain of PTP1E. Crystallographic and NMR evaluation of the impact of peptide binding to the second PDZ domain of PTP1E. Biochemistry. 2010 Sep 14; Authors: Zhang J, Sapienza PJ, Ke H, Chang A, Hengel SR, Wang H, Phillips GN, Lee AL PDZ (PSD95/Discs large/ZO-1) domains are ubiquitous protein interaction motifs found in scaffolding proteins involved in signal transduction. Despite the fact that many PDZs show a limited tendency to undergo structural change, the...
nmrlearner Journal club 0 09-16-2010 02:59 PM


Thread Tools Search this Thread
Search this Thread:

Advanced Search
Display Modes Rate This Thread
Rate This Thread:

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is On
Trackbacks are Off
Pingbacks are Off
Refbacks are Off



BioNMR advertisements to pay for website hosting and domain registration. Nobody does it for us.



Powered by vBulletin® Version 3.7.3
Copyright ©2000 - 2024, Jelsoft Enterprises Ltd.
Copyright, BioNMR.com, 2003-2013
Search Engine Friendly URLs by vBSEO 3.6.0

All times are GMT. The time now is 11:58 PM.


Map