poor signal to noise on 1H-15N heteronculear NOE
 

poor signal to noise on 1H-15N heteronculear NOE

I have recently attempted to run a 1H-15N heteronuclear NOE experiment in vnmrj (700 rt probe) using the standard sequence that comes with biopack, the signal to nose im getting compared to my standard HSQC is significantly reduced, is this normal? I do not recall this being the case when I ran this previously on a bruker 600 with RT probe? is this normal or does this suggest something is miss set? Also in the no NOE experiment I get very broad noise in the centre of my spectrum/water line.

regards

Tom



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