Question from NMRWiki Q&A forum Please suggest experiments that push the power limits of NMR probes

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[Question from NMRWiki Q&A forum] Please suggest experiments that push the power limits of NMR probes

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NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

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10-08-2010, 02:35 AM

nmrlearner

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Please suggest experiments that push the power limits of NMR probes

Please suggest experiments that push the power limits of NMR probes

I'm trying to come up with reasonable tests of probe performance for all channels by simulating conditions at which arcing is most likely to occur.

For those tests I usually read voltage of the incoming wave on the RF line using directional coupler with a 50dB attenuated pickup and oscilloscope after running an "experiment" pulsing at the power and duty cycle that simulates the condition to be tested. If the waveform looks nice and square - there is no arcing.

What experiments should I look into? OK for 1H I'd pick TOCSY with the longest mixing time we use.What about 13C, 15N, 31P?

How do you do this kind of testing for solids probes?

How do you test fitness of gradient coils?

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