Question from NMRWiki Q&A forum optimization of mixing time 3D NOESY experments

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[Question from NMRWiki Q&A forum] optimization of mixing time 3D NOESY experments

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NMR processing:

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Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

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GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

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Torsion angles from chemical shifts:

Preditor

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Promega- Proline

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CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

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RCI

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HADDOCK

Chemical shifts re-referencing:

NMR model quality:

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SAVES2 or SAVES4

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CH3shift- Methyl

ArShift- Aromatic

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Proshift

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Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

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CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

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09-21-2011, 05:25 AM

nmrlearner

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optimization of mixing time 3D NOESY experments

optimization of mixing time 3D NOESY experments

Could you please explain that how to optimize mixing time for 3D NOESY experiments for protein like C13 edit NOESY and N15 EDIT NOESY and i want keep spin diffusion as much as less ?

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