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[Question from NMRWiki Q&A forum] optimising o1 in solid state nmr

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NMR processing:

MDD

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Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

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FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

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Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

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03-09-2011, 04:19 AM

nmrlearner

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optimising o1 in solid state nmr

optimising o1 in solid state nmr

I'm a little bit confused about setting o1, in the case of a solid sample First o1 is the offset of the transmitter frequency so sfo1 = BF1 + o1In my case I have to set Sfo1 = BF1 so I must set o1 = 0 HzSecond there is another way to optimize o1 whish is by applying gs and looking the fid, adjusting o1 until we have a good exponential decrease of the fid. In this case o1 value may not be 0 Hz.Does the second technique is valid in solid stat experiences?And which one I have to use?

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