HSQC perturbation
HSQC perturbation
Dear All, I am trying to determine the interaction between 2 proteins. Is there any reference or literature which report the determination of binding constant between 2 proteins in which intensity drop of HSQC cross peaks can be used as the measuring parameter. As when I titrate these 2 proteins only change which I observe is intensity drop. I tried other biophysical techniques to determine binding constant, but results are not satisfactory. Any suggestion would be useful Regards Arun Check if somebody has answered this question on NMRWiki QA forum |
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