Hi all, I have a small question on how to aquired a complete signal in BRUKER AVIII.I want to create a multi pulse sequence (1D), where I want to aquire the signal (sequentially) do not adding or replacing the data before each pulse.
tp tp tp tp ...Performing Aquisition all time.................
|| - pulse
tp -time between pulse
When I try to use a simple loop in the most simplest case of the bruker's sequence 'onepulse', always I obtain is a adding or a replacing of the previous signal.
I'm still new in programming pulse sequences, I've been try to understand this problem with the Bruker's pulse sequence manual, and I believe this could be simple..
Any help will be appreciated.Thank you
Check if somebody has answered this question on NMRWiki QA forum
Did you find this post helpful? |
Similar Threads
Thread
Thread Starter
Forum
Replies
Last Post
[NMR paper] Multi-phosphorylation of the Intrinsically Disordered Unique Domain of c-Src Studied by In-Cell and Real-Time NMR Spectroscopy.
Multi-phosphorylation of the Intrinsically Disordered Unique Domain of c-Src Studied by In-Cell and Real-Time NMR Spectroscopy.
Related Articles Multi-phosphorylation of the Intrinsically Disordered Unique Domain of c-Src Studied by In-Cell and Real-Time NMR Spectroscopy.
Chembiochem. 2013 Jun 6;
Authors: Amata I, Maffei M, Igea A, Gay M, Vilaseca M, Nebreda AR, Pons M
Abstract
Intrinsically disordered regions (IDRs) are preferred sites for post-translational modifications essential for regulating protein function. The enhanced local...
nmrlearner
Journal club
0
06-08-2013 02:18 PM
[NMR paper] Pulse design for broadband correlation NMR spectroscopy by multi-rotating frames.
Pulse design for broadband correlation NMR spectroscopy by multi-rotating frames.
Pulse design for broadband correlation NMR spectroscopy by multi-rotating frames.
J Biomol NMR. 2013 Feb 19;
Authors: Coote P, Arthanari H, Yu TY, Natarajan A, Wagner G, Khaneja N
Abstract
We present a method for designing radio-frequency (RF) pulses for broadband or multi-band isotropic mixing at low power, suitable for protein NMR spectroscopy. These mixing pulses are designed analytically, rather than by numerical optimization, by repeatedly...
nmrlearner
Journal club
0
02-20-2013 06:24 PM
Parallel acquisition of multi-dimensional spectra in protein NMR
Parallel acquisition of multi-dimensional spectra in protein NMR
Abstract We introduce the use of multiple receivers applied in parallel for simultaneously recording multi-dimensional data sets of proteins in a single experiment. The utility of the approach is established through the introduction of the 2D 15N,1HN||13CO HSQC experiment in which a pair of two-dimensional 15N,1HN and 15N,13CO spectra are recorded. The methodology is further extended to higher dimensionality via the 3D 1HN||13CO HNCA in which a pair of data sets recording 13Cα,15N,1HN and 13Cα,15N,13CO chemical shifts...
nmrlearner
Journal club
0
07-20-2012 11:13 PM
[NMR analysis blog] Micropost: Pulse Sequence Blues!
Micropost: Pulse Sequence Blues!
Now that ENC is practically around the corner, here is a link that shows how far this conference encourages the participants’ creativity:
Pulse Sequence Blues!
NMR can also be fun :-)
https://blogger.googleusercontent.com/tracker/6953549091784501422-8328472542836523761?l=nmr-analysis.blogspot.com
nmrlearner
News from NMR blogs
0
02-23-2011 11:21 PM
[NMR analysis blog] Micropost: Pulse Sequence Blues!
Micropost: Pulse Sequence Blues!
Now that ENC is practically around the corner, here is a link that shows how far this conference encourages the participants’ creativity:
Pulse Sequence Blues!
NMR can also be fun :-)
https://blogger.googleusercontent.com/tracker/6953549091784501422-8328472542836523761?l=nmr-analysis.blogspot.com
[Question from NMRWiki Q&A forum] Best pulse sequence for HSQC in Guanidine-HCl
Best pulse sequence for HSQC in Guanidine-HCl
Hello,
I would like to acquire a series of HSQC spectra of an 15N-labeled 12 kDa protein unfolding (over the course of hours) in 3M GuaHCl. I have access to a Varian 600 spectrometer with a conventional probe. Due to the high ionic strength of the sample, tuning and shimming is a little difficult. However, I can record a sort-of-decent HSQC using a WaterGate gradient enhanced WGgNhsqc sequence. I am limited to using a protein concentration of about 250 uM. I've determined the pw90 to be 12.125 at a tpwr of 63.
Using WGgNhsqc, the protein...
nmrlearner
News from other NMR forums
0
10-23-2010 07:42 AM
Combining methods for speeding up multi-dimensional acquisition. Sparse sampling and
Combining methods for speeding up multi-dimensional acquisition. Sparse sampling and fast pulsing methods for unfolded proteins
Publication year: 2010
Source: Journal of Magnetic Resonance, In Press, Accepted Manuscript, Available online 12 June 2010</br>
Dominique, Marion</br>
Resonance assignment of intrinsically disordered proteins is made difficult by the extensive spectral overlaps. High-resolution 3D and 4D spectra are thus essential for this purpose. We have adapted the series of 3D BEST experiments proposed by Lescop et al to the case of unfolded proteins. Longer acquisitions...
How to performing acquisition during all time multi pulse sequence - Bruker ?
Linear Mode
