how many percent peak in NH HSQC enough to solve structure?
 

how many percent peak in NH HSQC enough to solve structure?

Hello everyone, I have a stupid question and often confuse me. We often meet peak missing in 15N H HSQC,but usually how many percent peak enough to solve NMR solution structure? I ask a lot of people and check in literature. Some guy suggested at least 80 except proline, other answer is at least 90%?For my current project, only appear 80% peak except proline. Now I am trying to optimize sample condition to make more peak. But I need to know a percent that I must optimize for my sample peak. Check my biology result I know that most missing peak lie in a very important disorder, which play an important function role. May I have a understand, If we can check missing peak. If miss peak have no important function, we don't care and directly to assign other peak and solve structure. If missing peak have function, it is a totally different condition. For my protein, If I can't make the disorder(misssing peak), I will not get the correct structure. Thanks a lot!

Chengkun Wang



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