Question from NMRWiki Q&A forum How important is calibrating a 90degree pulse for 2D?

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[Question from NMRWiki Q&A forum] How important is calibrating a 90degree pulse for 2D?

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06-28-2012, 08:21 PM

nmrlearner

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How important is calibrating a 90degree pulse for 2D?

How important is calibrating a 90degree pulse for 2D?

So it is pretty much always recommended to calibrate the 90 for 1H for any 2D experiment... Cosy, Noesy, HSQC, etc...

But when I have calibrated them, there is relatively little change... say from 7.32 to 7.65... Of course for all I know that could be a significant change...

Anyway, how 'off' does the 90 need to be to make a noticeable difference? If you have a concentrated sample, is it less sensitive to the 90?

Thanks!!

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