How to get off resonance ligand integral in STD experiment
 

How to get off resonance ligand integral in STD experiment

Hello I am using satxfer1D experiment on varian unity plus 500 MHz spectrometer and vnmr (not vnmrJ!) software. I would like to determine dissociation constant from STD amplification factor (Io-Isat)/Io. So I need separate spectrum saturated at 30ppm to get off-resonance ligand integrals Io. Standard STD experiment give me only the difference between on- and off- resonance spectra (Io-Isat). Is the possibility to extract or to programm satxfer1D experiment to get what I want?



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