BioNMR
NMR aggregator & online community since 2003
BioNMR    
Learn or help to learn NMR - get free NMR books!
 

Go Back   BioNMR > NMR community > News from other NMR forums
Advanced Search



Jobs Groups Conferences Literature Pulse sequences Software forums Programs Sample preps Web resources BioNMR issues


Webservers
NMR processing:
MDD
NMR assignment:
Backbone:
Autoassign
MARS
UNIO Match
PINE
Side-chains:
UNIO ATNOS-Ascan
NOEs:
UNIO ATNOS-Candid
UNIO Candid
ASDP
Structure from NMR restraints:
Ab initio:
GeNMR
Cyana
XPLOR-NIH
ASDP
UNIO ATNOS-Candid
UNIO Candid
Fragment-based:
BMRB CS-Rosetta
Rosetta-NMR (Robetta)
Template-based:
GeNMR
I-TASSER
Refinement:
Amber
Structure from chemical shifts:
Fragment-based:
WeNMR CS-Rosetta
BMRB CS-Rosetta
Homology-based:
CS23D
Simshift
Torsion angles from chemical shifts:
Preditor
TALOS
Promega- Proline
Secondary structure from chemical shifts:
CSI (via RCI server)
TALOS
MICS caps, β-turns
d2D
PECAN
Flexibility from chemical shifts:
RCI
Interactions from chemical shifts:
HADDOCK
Chemical shifts re-referencing:
Shiftcor
UNIO Shiftinspector
LACS
CheckShift
RefDB
NMR model quality:
NOEs, other restraints:
PROSESS
PSVS
RPF scores
iCing
Chemical shifts:
PROSESS
CheShift2
Vasco
iCing
RDCs:
DC
Anisofit
Pseudocontact shifts:
Anisofit
Protein geomtery:
Resolution-by-Proxy
PROSESS
What-If
iCing
PSVS
MolProbity
SAVES2 or SAVES4
Vadar
Prosa
ProQ
MetaMQAPII
PSQS
Eval123D
STAN
Ramachandran Plot
Rampage
ERRAT
Verify_3D
Harmony
Quality Control Check
NMR spectrum prediction:
FANDAS
MestReS
V-NMR
Flexibility from structure:
Backbone S2
Methyl S2
B-factor
Molecular dynamics:
Gromacs
Amber
Antechamber
Chemical shifts prediction:
From structure:
Shiftx2
Sparta+
Camshift
CH3shift- Methyl
ArShift- Aromatic
ShiftS
Proshift
PPM
CheShift-2- Cα
From sequence:
Shifty
Camcoil
Poulsen_rc_CS
Disordered proteins:
MAXOCC
Format conversion & validation:
CCPN
From NMR-STAR 3.1
Validate NMR-STAR 3.1
NMR sample preparation:
Protein disorder:
DisMeta
Protein solubility:
camLILA
ccSOL
Camfold
camGroEL
Zyggregator
Isotope labeling:
UPLABEL
Solid-state NMR:
sedNMR


Reply
Thread Tools Search this Thread Rate Thread Display Modes
  #1  
Unread 11-28-2013, 05:18 PM
nmrlearner's Avatar
Senior Member
 
Join Date: Jan 2005
Posts: 23,135
Points: 193,617, Level: 100
Points: 193,617, Level: 100 Points: 193,617, Level: 100 Points: 193,617, Level: 100
Level up: 0%, 0 Points needed
Level up: 0% Level up: 0% Level up: 0%
Activity: 50.7%
Activity: 50.7% Activity: 50.7% Activity: 50.7%
Last Achievements
Award-Showcase
NMR Credits: 0
NMR Points: 193,617
Downloads: 0
Uploads: 0
Default How to adjust the receiver gain in a deuterium oxide protein sample?

How to adjust the receiver gain in a deuterium oxide protein sample?

I am running a 15N HSQC on a protein sample which is in a 100% deuterium oxide solvent. The command rga in TopSpin gives a value of >10000 for the receiver gain. I understand this is happening because there is no water signal and hence the receiver gain is optimizing the S/N. However, should the experiment be run with that high value or should it be adjusted to a value of a size similar to the one used on water samples?



Check if somebody has answered this question on NMRWiki QA forum
Reply With Quote


Did you find this post helpful? Yes | No

Reply
Similar Threads
Thread Thread Starter Forum Replies Last Post
[Question from NMRWiki Q&A forum] Sensitivity gain by utilizing steady state polarization
Sensitivity gain by utilizing steady state polarization What do we exactly mean by "utilizing steady state polarization" to gain sensitivity in an experiment?...I know that steady state should be achieved before the start of acquisition which means, in a way, it is already being used for 1H,13C/15N(isn't it?)...I am lost in understanding this concept!...also, how/why does it differ in standard pulse sequences and spin state selective coherence transfer experiments? Check if somebody has answered this question on NMRWiki QA forum
nmrlearner News from other NMR forums 0 08-05-2013 09:06 AM
UW Students Gain Research Experience as Undergraduates - University of Wyoming News
http://www.bionmr.com//nt3.ggpht.com/news/tbn/TwnxFHMkKxrmoM/6.jpg University of Wyoming News <img alt="" height="1" width="1" /> UW Students Gain Research Experience as Undergraduates University of Wyoming News They use Nuclear Magnetic Resonance spectroscopy and other biophysical methods to study protein structure and function. Moulton is a member of the American Chemical Society, and has been recognized as a "Superior Student" in the UW Department of ... UW Students Gain Research Experience as Undergraduates - University of Wyoming News
nmrlearner Online News 0 04-20-2013 02:07 AM
[U. of Ottawa NMR Facility Blog] Receiver Gain and Signal-to-Noise Ratio
Receiver Gain and Signal-to-Noise Ratio The signal-to-noise ratio in an NMR spectrum can be affected drastically the choice of the receiver gain setting, so care should be taken to set the receiver gain correctly for optimum results. At very low receiver gain settings, both the signal and the noise use only a fraction of the available digitization levels of the analog-to-digital concertor (ADC). As a result, the intensity of each point in the FID is represented with only a few possible values and the FID is "choppy". This is analogous to a black and white photograph being represented...
nmrlearner News from NMR blogs 0 02-15-2013 04:27 AM
[Question from NMRWiki Q&A forum] 2DNOESY with N15 C13 Labelled sample or Unlabelled sample
2DNOESY with N15 C13 Labelled sample or Unlabelled sample Dear Friends, I am in process of doing structure calculation of dimeric protein 22kD by using ARIA2.2 . Can I acquire 2DNOESY spectrum for my protein. Will it provide useful information for structure calculation? And whether I should acquire 2DNOESY with N15 C13 Labelled sample or Unlabelled sample . Which sample would be appropriate for this kind of experiment on VNMRJ 700 with biopack ? Thanks & Regards Srinivas Check if somebody has answered this question on NMRWiki QA forum
nmrlearner News from other NMR forums 0 02-06-2012 03:54 PM
Structure of Peptides on Metal Oxide Surfaces Probed by NMR
Structure of Peptides on Metal Oxide Surfaces Probed by NMR Peter A. Mirau, Rajesh R. Naik and Patricia Gehring http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja205454t/aop/images/medium/ja-2011-05454t_0008.gif Journal of the American Chemical Society DOI: 10.1021/ja205454t http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA http://feeds.feedburner.com/~r/acs/jacsat/~4/twbT3VIr8Xo
nmrlearner Journal club 0 10-22-2011 10:16 AM
[Question from NMRWiki Q&A forum] Switiching off the receiver during acquisition
Switiching off the receiver during acquisition Hello nmr wikier. I use Bruker Avance I AQS 400. TopSpin Version 1.3 For a test I want to switch off the receiver during the acquisition. In pursuance of the pulse programming manual I can do that with the statement setrtp. So I wrote following pulse programm (excerpt): 1 ze 2 30m p1 ph1 3 DE1 DE2 DERX DEADC DEPA DE3 4 0.1u DWELL_GEN:f1
nmrlearner News from other NMR forums 0 09-13-2011 09:15 PM
[NMRwiki tweet] nmrwiki: How to adjust topshim parameters? #nmr @bruker http://t.co/9lopPjA
nmrwiki: How to adjust topshim parameters? #nmr @bruker http://t.co/9lopPjA nmrwiki: How to adjust topshim parameters? #nmr @bruker http://t.co/9lopPjA Source: NMRWiki tweets
nmrlearner Twitter NMR 0 07-25-2011 11:10 PM
[Question from NMRWiki Q&A forum] Should I decouple deuterium on 2H/15N labeled sample for R1, R2 and hetnoe?
Should I decouple deuterium on 2H/15N labeled sample for R1, R2 and hetnoe? If I'm running relaxation experiments (15N) for R1, R2, and hetnoe on a double labeled (2H/15N) sample, do I need to do any deuterium decoupling added to the program? Check if somebody has answered this question on NMRWiki QA forum
nmrlearner News from other NMR forums 0 08-22-2010 02:30 AM


Thread Tools Search this Thread
Search this Thread:

Advanced Search
Display Modes Rate This Thread
Rate This Thread:

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is On
Trackbacks are Off
Pingbacks are Off
Refbacks are Off



BioNMR advertisements to pay for website hosting and domain registration. Nobody does it for us.



Powered by vBulletin® Version 3.7.3
Copyright ©2000 - 2024, Jelsoft Enterprises Ltd.
Copyright, BioNMR.com, 2003-2013
Search Engine Friendly URLs by vBSEO 3.6.0

All times are GMT. The time now is 07:30 AM.


Map