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nmrlearner

06-06-2012 06:03 AM

Finding concentration from NMR spectrum

Finding concentration from NMR spectrum

I dissolve Aspartate in a buffer containing 2mM TSP. I have a NMR spectrum of the mixture and I need to find the concentration for the Aspartate.

The 1D spectrum shows 7 peaks at (ppm):0.00 (0.505), 0.60 (0.106), 1.75 (0.124), 2.62 (1.012), 2.80 (1.000), 3.65 (0.034), 3.80 (1.000)

What is the best way to find the concentration?

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