c13 edit noesy phasing problem
 

c13 edit noesy phasing problem

Dear NMR Wiki'ers

I Have acquired c13-edit noesy experiment on 700vnmr for my target protein (20kd) with mixing time of 120ms , I tried my best to correct the phasing, still it seems to be negative contours ,Peak broadening and noise is more after processing .How to overcome this problem ?

Fig-1 1D projections fig-2 f1f3 projection in process in t1fig-3 f1f3 projection in process in t1 after transformation (1026-512-256)

<img width=50% src="http://qa.nmrwiki.org/upfiles/13204074491921939.png" /img>



Check if somebody has answered this question on NMRWiki QA forum