Hi, thank you for reading my question. I am a novice at NMR, I apologize in advance if my question appears naive. I am synthesizing peptides composed solely of Lys and Leu residues in either a block or random orientation. To demonstrate the orientation, I am proposing to use 13C NMR. My expectation is the block orientation will demonstrate higher intensity carbonyl peaks for Lys-Lys and Leu-Leu than Lys-Leu whereas the random peptide will show a higher intensity peak for the Lys-Leu. Does this sound reasonable or does anyone have any other suggestions?
Check if somebody has answered this question on NMRWiki QA forum
Did you find this post helpful? |
Similar Threads
Thread
Thread Starter
Forum
Replies
Last Post
NMR for a 30-residue peptide
Dear all,
Is that possible to do structure determination of a 30-residue peptide
using 500 MHz NMR?
I have a 30-residue linear peptide with three Gly and two AHx
(aminohexanoic acid) groups at the center of the peptide. I read some
papers from people who use 500 MHz NMR to determine the structure of
up to 20-residue peptides, but not a 30-residue peptide.
bimo
NMR Questions and Answers
5
03-06-2013 12:35 AM
[CNS Yahoo group] Re: peptide bond cut during annealing
Re: peptide bond cut during annealing
Hi Nic, thanks a lot! This was the solution as you suggested: I forgot to add the new residue into the link file to be patched with PEPT. SInce the involved
More...
nmrlearner
News from other NMR forums
0
03-05-2012 04:29 PM
[CNS Yahoo group] Re: peptide bond cut during annealing
Re: peptide bond cut during annealing
Yes. I have had this happen when I had to create a novel amino acid (oxidized cysteine) and didn't set it up to 'link' with the normal amino acids.
More...
nmrlearner
News from other NMR forums
0
03-05-2012 04:29 PM
[CNS Yahoo group] peptide bond cut during annealing
peptide bond cut during annealing
Dear All, I was generated topologies/parameters for a new residue in a peptide. The generate.inp created a correct peptide bond from the new residue to the
More...
nmrlearner
News from other NMR forums
0
03-05-2012 04:04 AM
[CNS Yahoo group] Re: peptide bond cut during annealing
Re: peptide bond cut during annealing
Most likely, there is some error in topology. Please post your topology/parameter/link files for the unconventional residue.
More...
nmrlearner
News from other NMR forums
0
03-05-2012 04:04 AM
Transferred NOESY NMR studies of biotin mimetic peptide (FSHPQNT) bound to streptavidin: A structural model for studies of peptide-protein interactions.
Transferred NOESY NMR studies of biotin mimetic peptide (FSHPQNT) bound to streptavidin: A structural model for studies of peptide-protein interactions.
Transferred NOESY NMR studies of biotin mimetic peptide (FSHPQNT) bound to streptavidin: A structural model for studies of peptide-protein interactions.
Chem Biol Drug Des. 2011 Feb 5;
Authors: Gizachew D, Dratz E
Protein-protein interactions control signaling, specific adhesion and many other biological functions. The three dimensional structures of the interfaces and bound ligand can be...
nmrlearner
Journal club
0
02-08-2011 06:28 PM
[Question from NMRWiki Q&A forum] peptide protein interaction
peptide protein interaction
I have determined the structure of a 110 residues protein(11kDa) which is known to interact with a 15mer peptide. Now I am interested to know which residues of the 15mer peptide interacts with this 11kDa protein. Can anyone suggest a simple nmr experiment which can tell the residues from the 15mer peptide which interact with the protein.
Check if somebody has answered this question on NMRWiki QA forum
!3C NMR of Peptide
Linear Mode
