In some cases where there are baseline issues, automatic phase correction may not do a satisfactory job. It then becomes necessary to correct the phase manually. The following video demonstrates how to manually phase correct a 1D NMR spectrum in TOPSPIN.
[U. of Ottawa NMR Facility Blog] Removing t1 Noise from Homoonuclear 2D NMR Data - Video Tutorial
Removing t1 Noise from Homoonuclear 2D NMR Data - Video Tutorial
The often troublesome stripes of vertical noise in 2D NMR spectra are called t1 noise (i.e. noise originating in the t1 domain). When t1 noise occurs in homoonuclear 2D correlation experiments such as COSY, TOCSY, NOESY or ROESY, symmetrization can be used to remove a great deal of the noise and make the data more presentable. The technique was described in a previous post and is demonstrated in this video tutorial using a magnitude COSY spectrum as an example.
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nmrlearner
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04-05-2013 11:03 PM
[U. of Ottawa NMR Facility Blog] Removing t1 Noise from Heteronuclear 2D NMR Data - Video Tutorial
Removing t1 Noise from Heteronuclear 2D NMR Data - Video Tutorial
The often troublesome stripes of vertical noise in 2D NMR spectra are called t1 noise (i.e. noise originating in the t1 domain). When t1 noise occurs in hereronuclear 2D correlation experiments such as HMBC, HSQC, HMQC or HOESY, there is a simple trick to remove a great deal of the noise and make the data more presentable. The technique was described in a previous post and is demonstrated in this video tutorial using a 19F - 1H HOESY spectrum as an example.
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nmrlearner
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03-27-2013 03:03 AM
[Question from NMRWiki Q&A forum] Bruker & Varian phase correction parameters
Bruker & Varian phase correction parameters
Hi,
I am an undergraduate student who is doing a chemistry/computing project. I have chosen to write a simple C program that can import NMR data from commercial spectrometer software into a common format. The scope of my project is restricted to "1D Proton" as time is limited. I'm not sure if the code is going to get used for anything later (I doubt it) and I am not intending to do anything with my work other than get a good grade!
I have a question that relates to the phase correction parameters present in Bruker and Varian datasets:...
nmrlearner
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03-20-2013 01:47 PM
[U. of Ottawa NMR Facility Blog] Exponential Line Broadening - Video Tutorial
Exponential Line Broadening - Video Tutorial
Exponential line broadening is an important NMR data processing tool. It involves multiplying the time domain signal by a decaying exponential function prior to Fourier transforming the data into the frequency domain. It is used to improve the signal-to-noise ratio and is more fully described in a previous post. The following short tutorial video demonstrates its use.
http://4.bp.blogspot.com/-CozKHXyqcTA/UUdrJzrbyqI/AAAAAAAABG8/0FLtL9-4af0/s400/lb_vid.jpg
nmrlearner
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03-19-2013 12:58 AM
[U. of Ottawa NMR Facility Blog] Phasing a 2D NMR Spectrum - Video Tutorial
Phasing a 2D NMR Spectrum - Video Tutorial
The following video demonstrates how to phase a 2D NMR spectrum in TOPSPIN 3.
http://1.bp.blogspot.com/-CYsCw5IN6UQ/UUDc5lQptKI/AAAAAAAABGs/iFjKpU5cMoY/s400/2d_phasing_video.jpg
http://feeds.feedburner.com/~ff/UniversityOfOttawaNmrFacilityBlog?d=yIl2AUoC8zA
http://feeds.feedburner.com/~r/UniversityOfOttawaNmrFacilityBlog/~4/v9fPQ6e3bQw
Source: University of Ottawa NMR Facility Blog
nmrlearner
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03-13-2013 08:55 PM
Origin and removal of mixed-phase artifacts in gradient sensitivity enhanced heteronuclear single quantum correlation spectra
Origin and removal of mixed-phase artifacts in gradient sensitivity enhanced heteronuclear single quantum correlation spectra
Abstract Here we describe phasing anomalies observed in gradient sensitivity enhanced 15N-1H HSQC spectra, and analyze their origin. It is shown that, as a result of 15N off-resonance effects, dispersive contributions to the 1H signal become detectable, and lead to 15N-offset dependent phase errors. Strategies that effectively suppress these artifacts are presented.
Content Type Journal Article
Category Article
Pages 199-207
nmrlearner
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09-30-2011 08:01 PM
Sequence correction of random coil chemical shifts: correlation between neighbor correction factors and changes in the Ramachandran distribution
Sequence correction of random coil chemical shifts: correlation between neighbor correction factors and changes in the Ramachandran distribution
Abstract Random coil chemical shifts are necessary for secondary chemical shift analysis, which is the main NMR method for identification of secondary structure in proteins. One of the largest challenges in the determination of random coil chemical shifts is accounting for the effect of neighboring residues. The contributions from the neighboring residues are typically removed by using neighbor correction factors determined based on each...
nmrlearner
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06-06-2011 12:53 AM
Singular spectrum analysis for an automated solvent artifact removal and baseline correction of 1D NMR spectra.
Singular spectrum analysis for an automated solvent artifact removal and baseline correction of 1D NMR spectra.
Singular spectrum analysis for an automated solvent artifact removal and baseline correction of 1D NMR spectra.
J Magn Reson. 2011 Mar 6;
Authors: De Sanctis S, Malloni WM, Kremer W, Tomé AM, Lang EW, Neidig KP, Kalbitzer HR
NMR spectroscopy in biology and medicine is generally performed in aqueous solutions, thus in (1)H NMR spectroscopy, the dominant signal often stems from the partly suppressed solvent and can be many orders of...
Manual Phase Correction of 1D Spectra - Video Tutorial
Linear Mode
