The PRESS technique has been widely used to achieve voxel localization for in vivo 1H MRS acquisitions. However, for dynamic hyperpolarized 13C MRS experiments, the transition bands of the refocusing pulses may saturate the pre-polarized substrate spins flowing into the voxel. This limitation may be overcome by designing refocusing pulses that do not perturb the resonance of the hyperpolarized substrate, but selectively refocuses the spins of the metabolic products. In this study, a PRESS pulse sequence incorporating spectral–spatial refocusing pulses that have a stop band (‘notch’) at the substrate resonance is tested in vivo using hyperpolarized [1-13C]pyruvate. Higher metabolite SNR was observed in experiments using the spectral–spatial refocusing pulses as compared to conventional refocusing pulses.
Dynamic UltraFast 2D EXchange SpectroscopY (UF-EXSY) of hyperpolarized substrates
From The DNP-NMR Blog:
Dynamic UltraFast 2D EXchange SpectroscopY (UF-EXSY) of hyperpolarized substrates
Leon Swisher, C., et al., Dynamic UltraFast 2D EXchange SpectroscopY (UF-EXSY) of hyperpolarized substrates. J Magn Reson, 2015. 257: p. 102-9.
http://www.ncbi.nlm.nih.gov/pubmed/26117655
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09-10-2015 01:10 AM
Hybrid polarizing solids for pure hyperpolarized liquids through dissolution dynamic nuclear polarization
From The DNP-NMR Blog:
Hybrid polarizing solids for pure hyperpolarized liquids through dissolution dynamic nuclear polarization
Gajan, D., et al., Hybrid polarizing solids for pure hyperpolarized liquids through dissolution dynamic nuclear polarization. Proc. Nat. Aca. Sci. USA, 2014. 111(41): p. 14693-14697.
http://www.pnas.org/content/111/41/14693.abstract
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08-17-2015 07:21 PM
Hybrid polarizing solids for pure hyperpolarized liquids through dissolution dynamic nuclear polarization
From The DNP-NMR Blog:
Hybrid polarizing solids for pure hyperpolarized liquids through dissolution dynamic nuclear polarization
Gajan, D., et al., Hybrid polarizing solids for pure hyperpolarized liquids through dissolution dynamic nuclear polarization. Proc. Nat. Aca. Sci. USA, 2014. 111(41): p. 14693-14697.
http://www.pnas.org/content/111/41/14693.abstract
nmrlearner
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11-12-2014 03:02 PM
In vivo single-shot 13C spectroscopic imaging of hyperpolarized metabolites by spatiotemporal encoding
From The DNP-NMR Blog:
In vivo single-shot 13C spectroscopic imaging of hyperpolarized metabolites by spatiotemporal encoding
Schmidt, R., et al., In vivo single-shot 13C spectroscopic imaging of hyperpolarized metabolites by spatiotemporal encoding. J Magn Reson, 2014. 240(0): p. 8-15.
http://www.ncbi.nlm.nih.gov/pubmed/24486720
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06-02-2014 10:02 PM
In vivo single-shot C spectroscopic imaging of hyperpolarized metabolites by spatiotemporal encoding
From The DNP-NMR Blog:
In vivo single-shot C spectroscopic imaging of hyperpolarized metabolites by spatiotemporal encoding
Schmidt, R., et al., In vivo single-shot C spectroscopic imaging of hyperpolarized metabolites by spatiotemporal encoding. J Magn Reson, 2014. 240C(0): p. 8-15.
http://www.ncbi.nlm.nih.gov/pubmed/24486720
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02-19-2014 03:12 PM
Optimal variable flip angle schemes for dynamic acquisition of exchanging hyperpolarized substrates
From The DNP-NMR Blog:
Optimal variable flip angle schemes for dynamic acquisition of exchanging hyperpolarized substrates
Xing, Y., et al., Optimal variable flip angle schemes for dynamic acquisition of exchanging hyperpolarized substrates. J. Magn. Reson., 2013. 234(0): p. 75-81.
http://dx.doi.org/10.1016/j.jmr.2013.06.003
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11-21-2013 01:14 AM
Solution NMR of Polypeptides Hyperpolarized by Dynamic Nuclear Polarization.
Solution NMR of Polypeptides Hyperpolarized by Dynamic Nuclear Polarization.
Solution NMR of Polypeptides Hyperpolarized by Dynamic Nuclear Polarization.
Anal Chem. 2011 Jun 7;
Authors: Ragavan M, Chen HY, Sekar G, Hilty C
Hyperpolarization of nuclear spins through techniques such as Dynamic Nuclear Polarization (DNP) can greatly increase the signal to noise ratio in NMR measurements, thus eliminating the need for signal averaging. This enables the study of many dynamic processes which would otherwise not be amenable to study by NMR spectroscopy....
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Journal club
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06-10-2011 11:52 AM
[NMR paper] Structure and dynamic properties of the single disulfide-deficient alpha-amylase inhi
Structure and dynamic properties of the single disulfide-deficient alpha-amylase inhibitor tendamistat: an NMR study.
Related Articles Structure and dynamic properties of the single disulfide-deficient alpha-amylase inhibitor tendamistat: an NMR study.
Proteins. 1998 Nov 1;33(2):285-94
Authors: Balbach J, Seip S, Kessler H, Scharf M, Kashani-Poor N, Engels JW
Covalent linkages such as disulfide bonds are important for the stabilization of proteins. In the present NMR study we compare the structure and the dynamics of the single...
Single voxel localization for dynamic hyperpolarized 13C MR spectroscopy
Linear Mode
