Weber, E., et al., Sample Ripening through Nanophase Separation Impacts the Performance of Dynamic Nuclear Polarization. Angew. Chem., 2018: p. n/a-n/a.
Mixtures of water and glycerol provide popular matrices for low-temperature spectroscopy of vitrified samples. However, they involve counterintuitive physicochemical properties, such as spontaneous nanoscopic phase separations (NPS) in solutions that appear macroscopically homogeneous. We demonstrate that such phenomena can substantially impact the efficiency of dynamic nuclear polarization (DNP) by factors up to 20% by causing fluctuations in local concentrations of polarization agents (radicals). Thus, a spontaneous NPS of water/glycerol mixtures that takes place on time scales on the order of 30-60 min results in a confinement of polarization agents in nanoscopic water-rich vesicles, which in return affects the DNP. Such effects were found for three common polarization agents, TEMPOL, AMUPol and Trityl.
[NMR paper] Strategies for Efficient Sample Preparation for Dynamic Nuclear Polarization Solid-State NMR of Biological Macromolecules.
Strategies for Efficient Sample Preparation for Dynamic Nuclear Polarization Solid-State NMR of Biological Macromolecules.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--production.springer.de-OnlineResources-Logos-springerlink.gif Related Articles Strategies for Efficient Sample Preparation for Dynamic Nuclear Polarization Solid-State NMR of Biological Macromolecules.
Methods Mol Biol. 2018;1688:133-154
Authors: Itin B, Sergeyev IV
Abstract
Solid-state NMR (SSNMR) is a powerful tool for the elucidation...
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11-21-2017 10:10 PM
Hyperpolarization of nitrogen-15 nuclei by cross polarization and dissolution dynamic nuclear polarization
From The DNP-NMR Blog:
Hyperpolarization of nitrogen-15 nuclei by cross polarization and dissolution dynamic nuclear polarization
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Milani, J., et al., Hyperpolarization of nitrogen-15 nuclei by cross polarization and dissolution dynamic nuclear polarization. Review of Scientific Instruments, 2017. 88(1): p. 015109.
http://dx.doi.org/10.1063/1.4973777
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09-04-2017 03:11 PM
PRESTO polarization transfer to quadrupolar nuclei: implications for dynamic nuclear polarization
From The DNP-NMR Blog:
PRESTO polarization transfer to quadrupolar nuclei: implications for dynamic nuclear polarization
Perras, F.A., T. Kobayashi, and M. Pruski, PRESTO polarization transfer to quadrupolar nuclei: implications for dynamic nuclear polarization. Phys Chem Chem Phys, 2015. 17(35): p. 22616-22.
http://www.ncbi.nlm.nih.gov/pubmed/26266874
Role of Electron Spin Dynamics on Solid-State Dynamic Nuclear Polarization Performance
From The DNP-NMR Blog:
Role of Electron Spin Dynamics on Solid-State Dynamic Nuclear Polarization Performance
Siaw, T.A., et al., Role of Electron Spin Dynamics on Solid-State Dynamic Nuclear Polarization Performance. Phys. Chem. Chem. Phys., 2014.
http://dx.doi.org/10.1039/C4CP02013H
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06-20-2014 08:14 PM
Optimizing sample preparation methods for dynamic nuclear polarization solid-state NMR of synthetic polymers
From The DNP-NMR Blog:
Optimizing sample preparation methods for dynamic nuclear polarization solid-state NMR of synthetic polymers
Le, D., et al., Optimizing sample preparation methods for dynamic nuclear polarization solid-state NMR of synthetic polymers. Macromolecules, 2014: p. 140613123939001.
http://pubs.acs.org/doi/abs/10.1021/ma500788n
Construction and performance of an NMR tube with a sample cavity formed within magnetic susceptibility-matched glass.
Construction and performance of an NMR tube with a sample cavity formed within magnetic susceptibility-matched glass.
Construction and performance of an NMR tube with a sample cavity formed within magnetic susceptibility-matched glass.
J Magn Reson. 2011 Apr;209(2):167-73
Authors: Takeda M, Hallenga K, Shigezane M, Waelchli M, Löhr F, Markley JL, Kainosho M
We describe the construction and performance of an NMR tube with a magnetic susceptibility matched sample cavity that confines the solution within the detection zone in the axial direction and...
Sample Ripening through Nanophase Separation Impacts the Performance of Dynamic Nuclear Polarization
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