The often troublesome stripes of vertical noise in 2D NMR spectra are called t1 noise (i.e. noise originating in the t1 domain). When t1 noise occurs in hereronuclear 2D correlation experiments such as HMBC, HSQC, HMQC or HOESY, there is a simple trick to remove a great deal of the noise and make the data more presentable. The technique was described in a previous post and is demonstrated in this video tutorial using a 19F - 1H HOESY spectrum as an example.
[U. of Ottawa NMR Facility Blog] Exponential Line Broadening - Video Tutorial
Exponential Line Broadening - Video Tutorial
Exponential line broadening is an important NMR data processing tool. It involves multiplying the time domain signal by a decaying exponential function prior to Fourier transforming the data into the frequency domain. It is used to improve the signal-to-noise ratio and is more fully described in a previous post. The following short tutorial video demonstrates its use.
http://4.bp.blogspot.com/-CozKHXyqcTA/UUdrJzrbyqI/AAAAAAAABG8/0FLtL9-4af0/s400/lb_vid.jpg
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03-19-2013 12:58 AM
[U. of Ottawa NMR Facility Blog] Phasing a 2D NMR Spectrum - Video Tutorial
Phasing a 2D NMR Spectrum - Video Tutorial
The following video demonstrates how to phase a 2D NMR spectrum in TOPSPIN 3.
http://1.bp.blogspot.com/-CYsCw5IN6UQ/UUDc5lQptKI/AAAAAAAABGs/iFjKpU5cMoY/s400/2d_phasing_video.jpg
http://feeds.feedburner.com/~ff/UniversityOfOttawaNmrFacilityBlog?d=yIl2AUoC8zA
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Source: University of Ottawa NMR Facility Blog
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03-13-2013 08:55 PM
[U. of Ottawa NMR Facility Blog] Thermal Noise in NMR Data
Thermal Noise in NMR Data
The University of Ottawa has recently been funded for a 600 MHz NMR spectrometer with a cryogenetically cooled probe. Cryoprobes differ from conventional NMR probes in that the rf circuits and preamplifiers are cooled with cold helium gas while the sample is maintained at ambient temperature. The benefit of cryogenically cooled electronics compared to room temperature electronics is that the thermal noise in the system is reduced at cryogenic temperatures while the NMR signal remains constant for the sample at ambient temperature. The signal-to-noise ratio in...
[NMR paper] A mini-protein designed by removing a module from barnase: molecular modeling and NMR
A mini-protein designed by removing a module from barnase: molecular modeling and NMR measurements of the conformation.
Related Articles A mini-protein designed by removing a module from barnase: molecular modeling and NMR measurements of the conformation.
Protein Eng. 1999 Aug;12(8):673-80
Authors: Takahashi K, Noguti T, Hojo H, Yamauchi K, Kinoshita M, Aimoto S, Ohkubo T, G? M
A globular domain can be decomposed into compact modules consisting of contiguous 10-30 amino acid residues. The correlation between modules and exons observed in...
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11-18-2010 08:31 PM
[NMR paper] Combined use of 13C chemical shift and 1H alpha-13C alpha heteronuclear NOE data in m
Combined use of 13C chemical shift and 1H alpha-13C alpha heteronuclear NOE data in monitoring a protein NMR structure refinement.
Related Articles Combined use of 13C chemical shift and 1H alpha-13C alpha heteronuclear NOE data in monitoring a protein NMR structure refinement.
J Biomol NMR. 1995 Feb;5(2):161-72
Authors: Celda B, Biamonti C, Arnau MJ, Tejero R, Montelione GT
A large portion of the 13C resonance assignments for murine epidermal growth factor (mEGF) at pH 3.1 and 28 degrees C has been determined at natural isotope abundance....
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08-22-2010 03:41 AM
[Stan NMR blog] Noise Figure and Equivalent Input Noise
Noise Figure and Equivalent Input Noise
An educational article about the definitions of - and the relation between - the two concepts.
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Removing t1 Noise from Heteronuclear 2D NMR Data - Video Tutorial
Linear Mode
