BioNMR

BioNMR (http://www.bionmr.com/forum/)
-   News from NMR blogs (http://www.bionmr.com/forum/news-nmr-blogs-47/)
-   -   NMR-based structural biology enhanced by dynamic nuclear polarization at high magnetic field (http://www.bionmr.com/forum/news-nmr-blogs-47/nmr-based-structural-biology-enhanced-dynamic-nuclear-polarization-high-magnetic-field-21494/)

nmrlearner 11-19-2014 04:32 PM

NMR-based structural biology enhanced by dynamic nuclear polarization at high magnetic field
 
From The DNP-NMR Blog:

NMR-based structural biology enhanced by dynamic nuclear polarization at high magnetic field


Koers, E.J., et al., NMR-based structural biology enhanced by dynamic nuclear polarization at high magnetic field. J Biomol NMR, 2014. 60(2-3): p. 157-68.


http://www.ncbi.nlm.nih.gov/pubmed/25284462


Dynamic nuclear polarization (DNP) has become a powerful method to enhance spectroscopic sensitivity in the context of magnetic resonance imaging and nuclear magnetic resonance spectroscopy. We show that, compared to DNP at lower field (400 MHz/263 GHz), high field DNP (800 MHz/527 GHz) can significantly enhance spectral resolution and allows exploitation of the paramagnetic relaxation properties of DNP polarizing agents as direct structural probes under magic angle spinning conditions. Applied to a membrane-embedded K(+) channel, this approach allowed us to refine the membrane-embedded channel structure and revealed conformational substates that are present during two different stages of the channel gating cycle. High-field DNP thus offers atomic insight into the role of molecular plasticity during the course of biomolecular function in a complex cellular environment.


Go to The DNP-NMR Blog for more info.


All times are GMT. The time now is 08:07 AM.

Powered by vBulletin® Version 3.7.3
Copyright ©2000 - 2018, Jelsoft Enterprises Ltd.
Search Engine Friendly URLs by vBSEO 3.6.0
Copyright, BioNMR.com, 2003-2013