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nmrlearner 03-19-2018 02:52 PM

New NMR tools for protein structure and function: Spin tags for dynamic nuclear polarization solid state NMR #DNPNMR
 
From The DNP-NMR Blog:

New NMR tools for protein structure and function: Spin tags for dynamic nuclear polarization solid state NMR #DNPNMR

Rogawski, R. and A.E. McDermott, New NMR tools for protein structure and function: Spin tags for dynamic nuclear polarization solid state NMR. Arch. Biochem. Biophys., 2017. 628: p. 102-113.


https://www.ncbi.nlm.nih.gov/pubmed/28623034


Magic angle spinning solid state NMR studies of biological macromolecules [1-3] have enabled exciting studies of membrane proteins [4,5], amyloid fibrils [6], viruses, and large macromolecular assemblies [7]. Dynamic nuclear polarization (DNP) provides a means to enhance detection sensitivity for NMR, particularly for solid state NMR, with many recent biological applications and considerable contemporary efforts towards elaboration and optimization of the DNP experiment. This review explores precedents and innovations in biological DNP experiments, especially highlighting novel chemical biology approaches to introduce the radicals that serve as a source of polarization in DNP experiments.


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Go to The DNP-NMR Blog for more info.


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