On Mechanisms of Dynamic Nuclear Polarization in Solids

		BioNMR > NMR community > News from NMR blogs
On Mechanisms of Dynamic Nuclear Polarization in Solids

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

View First Unread

Thread Tools

Search this Thread

Rate Thread

Display Modes

02-25-2014, 12:44 AM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,174

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

On Mechanisms of Dynamic Nuclear Polarization in Solids

From The DNP-NMR Blog:

On Mechanisms of Dynamic Nuclear Polarization in Solids

Thurber, K.R. and R. Tycko, On Mechanisms of Dynamic Nuclear Polarization in Solids. Israel Journal of Chemistry, 2013: p. n/a-n/a.

http://dx.doi.org/10.1002/ijch.201300116

Dynamic nuclear polarization (DNP) can dramatically increase the signal in nuclear magnetic resonance (NMR) experiments by transferring spin polarization from electrons to nuclei. We discuss quantum mechanical descriptions of the “solid effect” and “cross effect” mechanisms of DNP that typically occur in solid state NMR at high magnetic field, both with and without magic angle spinning (MAS). We present several extensions of recent theoretical descriptions, including (i) treatments of solid effect DNP and cross effect DNP with MAS, in which two nuclei change spin states simultaneously and (ii) a treatment of solid effect DNP in a two-spin system without MAS, in which the ratio of microwave field strength to hyperfine coupling strength can be arbitrary. We also briefly discuss factors that influence DNP efficiency in experiments, beyond the basic principles contained in simple quantum mechanical models.

Go to The DNP-NMR Blog for more info.

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
[NMR paper] Dynamic nuclear polarization-enhanced 13C NMR spectroscopy of static biological solids. Dynamic nuclear polarization-enhanced 13C NMR spectroscopy of static biological solids. http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Dynamic nuclear polarization-enhanced 13C NMR spectroscopy of static biological solids. J Magn Reson. 2013 Jun;231:5-14 Authors: Potapov A, Yau WM, Tycko R Abstract We explore the possibility of using dynamic nuclear polarization (DNP) to enhance signals in structural studies of biological solids by solid state NMR...	nmrlearner	Journal club	0	01-11-2014 07:50 PM
Cross Polarization for Dissolution Dynamic Nuclear Polarization Experiments at Readily Accessible Temperatures 1.2* From The DNP-NMR Blog: Cross Polarization for Dissolution Dynamic Nuclear Polarization Experiments at Readily Accessible Temperatures 1.2*	nmrlearner	News from NMR blogs	0	11-21-2013 01:14 AM
Theoretical Aspects of Dynamic Nuclear Polarization in the Solid State: The Influence of High Radical Concentrations on the Solid Effect and Cross Effect Mechanisms From The DNP-NMR Blog: Theoretical Aspects of Dynamic Nuclear Polarization in the Solid State: The Influence of High Radical Concentrations on the Solid Effect and Cross Effect Mechanisms Hovav, Y., et al., Theoretical Aspects of Dynamic Nuclear Polarization in the Solid State: The Influence of High Radical Concentrations on the Solid Effect and Cross Effect Mechanisms. Appl. Magn. Reson., 2012. 43(1-2): p. 21-41. http://dx.doi.org/10.1007/s00723-012-0359-0	nmrlearner	News from NMR blogs	0	11-21-2013 01:14 AM
Dynamic nuclear polarization methods in solids and solutions to explore membrane proteins and membrane systems From The DNP-NMR Blog: Dynamic nuclear polarization methods in solids and solutions to explore membrane proteins and membrane systems Cheng, C.Y. and S. Han, Dynamic nuclear polarization methods in solids and solutions to explore membrane proteins and membrane systems. Annu Rev Phys Chem, 2013. 64(1): p. 507-32. http://www.ncbi.nlm.nih.gov/pubmed/23331309	nmrlearner	News from NMR blogs	0	07-08-2013 02:17 PM
Dynamic nuclear polarization-enhanced 13C NMR spectroscopy of static biological solids From The DNP-NMR Blog: Dynamic nuclear polarization-enhanced 13C NMR spectroscopy of static biological solids Potapov, A., W.M. Yau, and R. Tycko, Dynamic nuclear polarization-enhanced (13)C NMR spectroscopy of static biological solids. J Magn Reson, 2013. 231(0): p. 5-14. http://www.ncbi.nlm.nih.gov/pubmed/23562665	nmrlearner	News from NMR blogs	0	06-25-2013 12:17 AM
Dynamic nuclear polarization-enhanced 13C NMR spectroscopy of static biological solids Dynamic nuclear polarization-enhanced 13C NMR spectroscopy of static biological solids Available online 27 February 2013 Publication year: 2013 Source:Journal of Magnetic Resonance</br> </br> We explore the possibility of using dynamic nuclear polarization (DNP) to enhance signals in structural studies of biological solids by solid state NMR without sample spinning. Specifically, we use 2D 13C-13C exchange spectroscopy to probe the peptide backbone torsion angles (?,?) in a series of selectively 13C-labeled 40-residue ?-amyloid (A ?1-40) samples, in both fibrillar and...	nmrlearner	Journal club	0	02-28-2013 07:57 PM
Fast passage dynamic nuclear polarization on rotating solids Fast passage dynamic nuclear polarization on rotating solids November 2012 Publication year: 2012 Source:Journal of Magnetic Resonance, Volume 224</br> </br> Magic Angle Spinning (MAS) Dynamic Nuclear Polarization (DNP) has proven to be a very powerful way to improve the signal to noise ratio of NMR experiments on solids. The experiments have in general been interpreted considering the Solid-Effect (SE) and Cross-Effect (CE) DNP mechanisms while ignoring the influence of sample spinning. In this paper, we show experimental data of MAS-DNP enhancements of 1H and 13C in...	nmrlearner	Journal club	0	12-01-2012 06:10 PM
Fast Passage Dynamic Nuclear Polarization on Rotating Solids Fast Passage Dynamic Nuclear Polarization on Rotating Solids Publication year: 2012 Source:Journal of Magnetic Resonance</br> Frederic Mentink-Vigier, Ümit Akbey, Yonatan Hovav, Shimon Vega, Hartmut Oschkinat, Akiva Feintuch</br> Magic Angle Spinning (MAS) Dynamic Nuclear Polarization (DNP) has proven to be a very powerful way to improve the signal to noise ratio of NMR experiments on solids. The experiments have in general been interpreted considering the Solid-Effect (SE) and Cross-Effect (CE) DNP mechanisms while ignoring the influence of sample spinning. In this...	nmrlearner	Journal club	0	08-25-2012 08:56 AM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is Off Trackbacks are Off Pingbacks are Off Refbacks are Off