BioNMR

BioNMR (http://www.bionmr.com/forum/)
-   News from NMR blogs (http://www.bionmr.com/forum/news-nmr-blogs-47/)
-   -   Investigation of Intrinsically Disordered Proteins through Exchange with Hyperpolarized Water (http://www.bionmr.com/forum/news-nmr-blogs-47/investigation-intrinsically-disordered-proteins-through-exchange-hyperpolarized-water-24339/)

nmrlearner 02-28-2017 12:02 AM

Investigation of Intrinsically Disordered Proteins through Exchange with Hyperpolarized Water
 
From The DNP-NMR Blog:

Investigation of Intrinsically Disordered Proteins through Exchange with Hyperpolarized Water

p.p1 {margin: 0.0px 0.0px 0.0px 36.0px; text-indent: -36.0px; font: 12.0px Helvetica}
Kurzbach, D., et al., Investigation of Intrinsically Disordered Proteins through Exchange with Hyperpolarized Water. Angew. Chem. Int. Ed., 2017. 56(1): p. 389-392.


http://dx.doi.org/10.1002/anie.201608903


Hyperpolarized water can selectively enhance NMR signals of rapidly exchanging protons in osteopontin (OPN), a metastasis-associated intrinsically disordered protein (IDP), at near-physiological pH and temperature. The transfer of magnetization from hyperpolarized water is limited to solvent-exposed residues and therefore selectively enhances signals in 1H-15N correlation spectra. Binding to the polysaccharide heparin was found to induce the unfolding of preformed structural elements in OPN.
p.p1 {margin: 0.0px 0.0px 0.0px 0.0px; font: 12.0px Helvetica} p.p1 {margin: 0.0px 0.0px 0.0px 0.0px; font: 12.0px Helvetica}

Go to The DNP-NMR Blog for more info.


All times are GMT. The time now is 03:43 PM.

Powered by vBulletin® Version 3.7.3
Copyright ©2000 - 2024, Jelsoft Enterprises Ltd.
Search Engine Friendly URLs by vBSEO 3.6.0
Copyright, BioNMR.com, 2003-2013