Traditional (31)P NMR or MRI measurements suffer from low sensitivity relative to (1)H detection and consequently require longer scan times. We show here that hyperpolarization of (31)P nuclei through reversible interactions with parahydrogen can deliver substantial signal enhancements in a range of regioisomeric phosphonate esters containing a heteroaromatic motif which were synthesized in order to identify the optimum molecular scaffold for polarization transfer. A 3588-fold (31)P signal enhancement (2.34% polarization) was returned for a partially deuterated pyridyl substituted phosphonate ester. This hyperpolarization level is sufficient to allow single scan (31)P MR images of a phantom to be recorded at a 9.4 T observation field in seconds that have signal-to-noise ratios of up to 94.4 when the analyte concentration is 10 mM. In contrast, a 12 h 2048 scan measurement under standard conditions yields a signal-to-noise ratio of just 11.4. (31)P-hyperpolarized images are also reported from a 7 T preclinical scanner.
Improving protein solubility & long-term stability
Has anybody successfully used the following method?
A simple method for improving protein solubility and long-term stability.
Golovanov AP, Hautbergue GM, Wilson SA, Lian LY.
Department of Biomolecular Sciences, University of Manchester Institute of Science and Technology, P. O. Box 88, Manchester M60 1QD, UK. a.golovanov@umist.ac.jp
J Am Chem Soc. 2004 Jul 28;126(29):8933-9.
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Proteins
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01-22-2014 07:04 PM
Design of a 13C magnetic resonance probe using a deuterated methoxy group as a long-lived hyperpolarization unit
From the The DNP-NMR Blog:
Design of a 13C magnetic resonance probe using a deuterated methoxy group as a long-lived hyperpolarization unit
Doura, T., et al., Design of a 13C Magnetic Resonance Probe Using a Deuterated Methoxy Group as a Long-Lived Hyperpolarization Unit. Angew. Chem. Int. Ed., 2012. 51(40): p. 10114-10117.
http://www.ncbi.nlm.nih.gov/pubmed/22961955
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News from NMR blogs
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04-15-2013 08:52 AM
Grid computing for improving conformational sampling in NMR structure calculation.
Grid computing for improving conformational sampling in NMR structure calculation.
Grid computing for improving conformational sampling in NMR structure calculation.
Bioinformatics. 2011 May 5;
Authors: Mareuil F, Blanchet C, Malliavin TE, Nilges M
MOTIVATION: Methods for automatic NMR structure determination need to face a high level of ambiguity encountered in NMR spectra recorded by solid-state NMR and by solution NMR of partially unfolded proteins, leading to time-consuming calculations. The software package Ambiguous Restraints for Iterative...
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Journal club
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05-08-2011 04:35 AM
[NMR paper] Improving the accuracy of NMR structures of large proteins using pseudocontact shifts
Improving the accuracy of NMR structures of large proteins using pseudocontact shifts as long-range restraints.
Related Articles Improving the accuracy of NMR structures of large proteins using pseudocontact shifts as long-range restraints.
J Biomol NMR. 2004 Mar;28(3):205-12
Authors: Gaponenko V, Sarma SP, Altieri AS, Horita DA, Li J, Byrd RA
We demonstrate improved accuracy in protein structure determination for large (>/=30 kDa), deuterated proteins (e.g. STAT4(NT)) via the combination of pseudocontact shifts for amide and methyl protons...
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11-24-2010 09:25 PM
[NMR paper] The importance of being ordered: improving NMR structures using residual dipolar coup
The importance of being ordered: improving NMR structures using residual dipolar couplings.
Related Articles The importance of being ordered: improving NMR structures using residual dipolar couplings.
C R Biol. 2002 Sep;325(9):957-66
Authors: Gronenborn AM
Residual dipolar couplings arise from small degrees of alignment of molecules in a magnetic field. Most biomolecules lack sufficient intrinsic magnetic susceptibility anisotropies for practical purposes; however, alignment can be achieved using dilute aqueous phospholipid mixtures, colloidal...
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Journal club
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11-24-2010 08:58 PM
[NMR paper] Improving the quality of protein structures derived by NMR spectroscopy.
Improving the quality of protein structures derived by NMR spectroscopy.
Related Articles Improving the quality of protein structures derived by NMR spectroscopy.
J Biomol NMR. 2002 Mar;22(3):281-9
Authors: Spronk CA, Linge JP, Hilbers CW, Vuister GW
Biomolecular structures provide the basis for many studies in several research areas such as homology modelling, structure-based drug design and functional genomics. It is an important prerequisite that the structure is reliable in terms of accurate description of the experimental data, and in...
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11-24-2010 08:49 PM
[NMR paper] Improving the accuracy of NMR structures of DNA by means of a database potential of m
Improving the accuracy of NMR structures of DNA by means of a database potential of mean force describing base-base positional interactions.
Related Articles Improving the accuracy of NMR structures of DNA by means of a database potential of mean force describing base-base positional interactions.
J Am Chem Soc. 2001 May 2;123(17):3903-18
Authors: Kuszewski J, Schwieters C, Clore GM
NMR structure determination of nucleic acids presents an intrinsically difficult problem since the density of short interproton distance contacts is relatively low...
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11-19-2010 08:32 PM
[NMR paper] Improving the quality of NMR and crystallographic protein structures by means of a co
Improving the quality of NMR and crystallographic protein structures by means of a conformational database potential derived from structure databases.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_FREE_120x27.gif http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.pubmedcentral.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.gif Related Articles Improving the quality of NMR and crystallographic protein structures by means of a conformational database potential derived from structure databases.
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Improving the hyperpolarization of (31)P nuclei by synthetic design
Linear Mode
