Hyperpolarized Water to Study Protein–Ligand Interactions
From The DNP-NMR Blog:
Hyperpolarized Water to Study Protein–Ligand Interactions Chappuis, Q., et al., Hyperpolarized Water to Study Protein–Ligand Interactions. The Journal of Physical Chemistry Letters, 2015. 6(9): p. 1674-1678. http://dx.doi.org/10.1021/acs.jpclett.5b00403 The affinity between a chosen target protein and small molecules is a key aspect of drug discovery. Screening by popular NMR methods such as Water-LOGSY suffers from low sensitivity and from false positives caused by aggregated or denatured proteins. This work demonstrates that the sensitivity of Water-LOGSY can be greatly boosted by injecting hyperpolarized water into solutions of proteins and ligands. Ligand binding can be detected in a few seconds, whereas about 30 min is usually required without hyperpolarization. Hyperpolarized water also enhances proton signals of proteins at concentrations below 20 ?M so that one can verify in a few seconds whether the proteins remain intact or have been denatured Go to The DNP-NMR Blog for more info. |
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