BioNMR
NMR aggregator & online community since 2003
BioNMR    
Learn or help to learn NMR - get free NMR books!
 

Go Back   BioNMR > NMR community > News from NMR blogs
Advanced Search



Jobs Groups Conferences Literature Pulse sequences Software forums Programs Sample preps Web resources BioNMR issues


Webservers
NMR processing:
MDD
NMR assignment:
Backbone:
Autoassign
MARS
UNIO Match
PINE
Side-chains:
UNIO ATNOS-Ascan
NOEs:
UNIO ATNOS-Candid
UNIO Candid
ASDP
Structure from NMR restraints:
Ab initio:
GeNMR
Cyana
XPLOR-NIH
ASDP
UNIO ATNOS-Candid
UNIO Candid
Fragment-based:
BMRB CS-Rosetta
Rosetta-NMR (Robetta)
Template-based:
GeNMR
I-TASSER
Refinement:
Amber
Structure from chemical shifts:
Fragment-based:
WeNMR CS-Rosetta
BMRB CS-Rosetta
Homology-based:
CS23D
Simshift
Torsion angles from chemical shifts:
Preditor
TALOS
Promega- Proline
Secondary structure from chemical shifts:
CSI (via RCI server)
TALOS
MICS caps, β-turns
d2D
PECAN
Flexibility from chemical shifts:
RCI
Interactions from chemical shifts:
HADDOCK
Chemical shifts re-referencing:
Shiftcor
UNIO Shiftinspector
LACS
CheckShift
RefDB
NMR model quality:
NOEs, other restraints:
PROSESS
PSVS
RPF scores
iCing
Chemical shifts:
PROSESS
CheShift2
Vasco
iCing
RDCs:
DC
Anisofit
Pseudocontact shifts:
Anisofit
Protein geomtery:
Resolution-by-Proxy
PROSESS
What-If
iCing
PSVS
MolProbity
SAVES2 or SAVES4
Vadar
Prosa
ProQ
MetaMQAPII
PSQS
Eval123D
STAN
Ramachandran Plot
Rampage
ERRAT
Verify_3D
Harmony
Quality Control Check
NMR spectrum prediction:
FANDAS
MestReS
V-NMR
Flexibility from structure:
Backbone S2
Methyl S2
B-factor
Molecular dynamics:
Gromacs
Amber
Antechamber
Chemical shifts prediction:
From structure:
Shiftx2
Sparta+
Camshift
CH3shift- Methyl
ArShift- Aromatic
ShiftS
Proshift
PPM
CheShift-2- Cα
From sequence:
Shifty
Camcoil
Poulsen_rc_CS
Disordered proteins:
MAXOCC
Format conversion & validation:
CCPN
From NMR-STAR 3.1
Validate NMR-STAR 3.1
NMR sample preparation:
Protein disorder:
DisMeta
Protein solubility:
camLILA
ccSOL
Camfold
camGroEL
Zyggregator
Isotope labeling:
UPLABEL
Solid-state NMR:
sedNMR


Reply
Thread Tools Search this Thread Rate Thread Display Modes
  #1  
Unread 07-23-2014, 11:25 PM
nmrlearner's Avatar
Senior Member
 
Join Date: Jan 2005
Posts: 23,134
Points: 193,617, Level: 100
Points: 193,617, Level: 100 Points: 193,617, Level: 100 Points: 193,617, Level: 100
Level up: 0%, 0 Points needed
Level up: 0% Level up: 0% Level up: 0%
Activity: 50.7%
Activity: 50.7% Activity: 50.7% Activity: 50.7%
Last Achievements
Award-Showcase
NMR Credits: 0
NMR Points: 193,617
Downloads: 0
Uploads: 0
Default Hyperpolarized 13C dehydroascorbate as an endogenous redox sensor for in vivo metabolic imaging

From The DNP-NMR Blog:

Hyperpolarized 13C dehydroascorbate as an endogenous redox sensor for in vivo metabolic imaging


Keshari, K.R., et al., Hyperpolarized 13C dehydroascorbate as an endogenous redox sensor for in vivo metabolic imaging. Proc. Nat. Aca. Sci. USA, 2011. 108(46): p. 18606-18611.


http://www.pnas.org/content/108/46/18606.abstract


Reduction and oxidation (redox) chemistry is involved in both normal and abnormal cellular function, in processes as diverse as circadian rhythms and neurotransmission. Intracellular redox is maintained by coupled reactions involving NADPH, glutathione (GSH), and vitamin C, as well as their corresponding oxidized counterparts. In addition to functioning as enzyme cofactors, these reducing agents have a critical role in dealing with reactive oxygen species (ROS), the toxic products of oxidative metabolism seen as culprits in aging, neurodegenerative disease, and ischemia/ reperfusion injury. Despite this strong relationship between redox and human disease, methods to interrogate a redox pair in vivo are limited. Here we report the development of [1-13C] dehydroascorbate [DHA], the oxidized form of Vitamin C, as an endogenous redox sensor for in vivo imaging using hyperpolarized 13C spectroscopy. In murine models, hyperpolarized [1-13C] DHA was rapidly converted to [1-13C] vitamin C within the liver, kidneys, and brain, as well as within tumor in a transgenic prostate cancer mouse. This result is consistent with what has been previously described for the DHA/Vitamin C redox pair, and points to a role for hyperpolarized [1-13C] DHA in characterizing the concentrations of key intracellular reducing agents, including GSH. More broadly, these findings suggest a prognostic role for this new redox sensor in determining vulnerability of both normal and abnormal tissues to ROS.


Go to The DNP-NMR Blog for more info.
Reply With Quote


Did you find this post helpful? Yes | No

Reply
Similar Threads
Thread Thread Starter Forum Replies Last Post
In vivo single-shot 13C spectroscopic imaging of hyperpolarized metabolites by spatiotemporal encoding
From The DNP-NMR Blog: In vivo single-shot 13C spectroscopic imaging of hyperpolarized metabolites by spatiotemporal encoding Schmidt, R., et al., In vivo single-shot 13C spectroscopic imaging of hyperpolarized metabolites by spatiotemporal encoding. J Magn Reson, 2014. 240(0): p. 8-15. http://www.ncbi.nlm.nih.gov/pubmed/24486720
nmrlearner News from NMR blogs 0 06-02-2014 10:02 PM
In vivo single-shot C spectroscopic imaging of hyperpolarized metabolites by spatiotemporal encoding
From The DNP-NMR Blog: In vivo single-shot C spectroscopic imaging of hyperpolarized metabolites by spatiotemporal encoding Schmidt, R., et al., In vivo single-shot C spectroscopic imaging of hyperpolarized metabolites by spatiotemporal encoding. J Magn Reson, 2014. 240C(0): p. 8-15. http://www.ncbi.nlm.nih.gov/pubmed/24486720
nmrlearner News from NMR blogs 0 02-19-2014 03:12 PM
Postdoc position (in vivo hyperpolarized 13C MR)
From The DNP-NMR Blog: Postdoc position (in vivo hyperpolarized 13C MR) The University of Maryland School of Medicine is expanding its molecular imaging and interventional research capabilities with the addition of a Dynamic Nuclear Polarizer and High-Intensity Focused Ultrasound as part of the recently established Center for Integration of Metabolic Imaging and Therapeutics (CIMIT). This expansion is aimed to facilitate both basic science and clinical research by exploring novel molecular imaging agent based technologies for screening, early detection, and real-time...
nmrlearner News from NMR blogs 0 11-21-2013 01:14 AM
In vivo magnetic resonance imaging of hyperpolarized silicon particles
From The DNP-NMR Blog: In vivo magnetic resonance imaging of hyperpolarized silicon particles Cassidy, M.C., et al., In vivo magnetic resonance imaging of hyperpolarized silicon particles. Nat Nano, 2013. 8(5): p. 363-368. http://dx.doi.org/10.1038/nnano.2013.65
nmrlearner News from NMR blogs 0 08-29-2013 01:27 AM
Strategies for rapid in vivo 1H and hyperpolarized 13C MR spectroscopic imaging
From the The DNP-NMR Blog: Strategies for rapid in vivo 1H and hyperpolarized 13C MR spectroscopic imaging Nelson, S.J., et al., Strategies for rapid in vivo 1H and hyperpolarized 13C MR spectroscopic imaging. J. Magn. Reson., 2013. 229(0): p. 187-197. http://dx.doi.org/10.1016/j.jmr.2013.02.003
nmrlearner News from NMR blogs 0 04-15-2013 08:52 AM
Metabolic response of glioma to dichloroacetate measured in vivo by hyperpolarized 13C magnetic resonance spectroscopic imaging
From the The DNP-NMR Blog: Metabolic response of glioma to dichloroacetate measured in vivo by hyperpolarized 13C magnetic resonance spectroscopic imaging Park, J.M., et al., Metabolic response of glioma to dichloroacetate measured in vivo by hyperpolarized 13C magnetic resonance spectroscopic imaging. Neuro-Oncology, 2013. 15(4): p. 433-41. http://neuro-oncology.oxfordjournals.org/content/early/2013/01/16/neuonc.nos319.abstract
nmrlearner News from NMR blogs 0 04-15-2013 08:52 AM
Hyperpolarized (13) C-labelled anhydrides as DNP precursors of metabolic MRI agents
From the The DNP-NMR Blog: Hyperpolarized (13) C-labelled anhydrides as DNP precursors of metabolic MRI agents Colombo Serra, S., et al., Hyperpolarized 13C-labelled anhydrides as DNP precursors of metabolic MRI agents. Contrast Media & Molecular Imaging, 2012. 7(5): p. 469-477. http://www.ncbi.nlm.nih.gov/pubmed/22821881
nmrlearner News from NMR blogs 0 04-15-2013 08:52 AM
[Stan NMR blog] Mercury in-vivo imaging?
Mercury in-vivo imaging? Answer to a query about the feasibility of 199Hg MRI Source: Stan blog library
nmrlearner News from NMR blogs 0 12-21-2010 02:14 AM


Thread Tools Search this Thread
Search this Thread:

Advanced Search
Display Modes Rate This Thread
Rate This Thread:

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are Off
Pingbacks are Off
Refbacks are Off



BioNMR advertisements to pay for website hosting and domain registration. Nobody does it for us.



Powered by vBulletin® Version 3.7.3
Copyright ©2000 - 2024, Jelsoft Enterprises Ltd.
Copyright, BioNMR.com, 2003-2013
Search Engine Friendly URLs by vBSEO 3.6.0

All times are GMT. The time now is 10:07 AM.


Map