BioNMR
NMR aggregator & online community since 2003
BioNMR    
Learn or help to learn NMR - get free NMR books!
 

Go Back   BioNMR > NMR community > News from NMR blogs
Advanced Search



Jobs Groups Conferences Literature Pulse sequences Software forums Programs Sample preps Web resources BioNMR issues


Webservers
NMR processing:
MDD
NMR assignment:
Backbone:
Autoassign
MARS
UNIO Match
PINE
Side-chains:
UNIO ATNOS-Ascan
NOEs:
UNIO ATNOS-Candid
UNIO Candid
ASDP
Structure from NMR restraints:
Ab initio:
GeNMR
Cyana
XPLOR-NIH
ASDP
UNIO ATNOS-Candid
UNIO Candid
Fragment-based:
BMRB CS-Rosetta
Rosetta-NMR (Robetta)
Template-based:
GeNMR
I-TASSER
Refinement:
Amber
Structure from chemical shifts:
Fragment-based:
WeNMR CS-Rosetta
BMRB CS-Rosetta
Homology-based:
CS23D
Simshift
Torsion angles from chemical shifts:
Preditor
TALOS
Promega- Proline
Secondary structure from chemical shifts:
CSI (via RCI server)
TALOS
MICS caps, β-turns
d2D
PECAN
Flexibility from chemical shifts:
RCI
Interactions from chemical shifts:
HADDOCK
Chemical shifts re-referencing:
Shiftcor
UNIO Shiftinspector
LACS
CheckShift
RefDB
NMR model quality:
NOEs, other restraints:
PROSESS
PSVS
RPF scores
iCing
Chemical shifts:
PROSESS
CheShift2
Vasco
iCing
RDCs:
DC
Anisofit
Pseudocontact shifts:
Anisofit
Protein geomtery:
Resolution-by-Proxy
PROSESS
What-If
iCing
PSVS
MolProbity
SAVES2 or SAVES4
Vadar
Prosa
ProQ
MetaMQAPII
PSQS
Eval123D
STAN
Ramachandran Plot
Rampage
ERRAT
Verify_3D
Harmony
Quality Control Check
NMR spectrum prediction:
FANDAS
MestReS
V-NMR
Flexibility from structure:
Backbone S2
Methyl S2
B-factor
Molecular dynamics:
Gromacs
Amber
Antechamber
Chemical shifts prediction:
From structure:
Shiftx2
Sparta+
Camshift
CH3shift- Methyl
ArShift- Aromatic
ShiftS
Proshift
PPM
CheShift-2- Cα
From sequence:
Shifty
Camcoil
Poulsen_rc_CS
Disordered proteins:
MAXOCC
Format conversion & validation:
CCPN
From NMR-STAR 3.1
Validate NMR-STAR 3.1
NMR sample preparation:
Protein disorder:
DisMeta
Protein solubility:
camLILA
ccSOL
Camfold
camGroEL
Zyggregator
Isotope labeling:
UPLABEL
Solid-state NMR:
sedNMR


Reply
Thread Tools Search this Thread Rate Thread Display Modes
  #1  
Unread 08-21-2010, 08:15 PM
nmrlearner's Avatar
Senior Member
 
Join Date: Jan 2005
Posts: 18,698
Points: 193,617, Level: 100
Points: 193,617, Level: 100 Points: 193,617, Level: 100 Points: 193,617, Level: 100
Level up: 0%, 0 Points needed
Level up: 0% Level up: 0% Level up: 0%
Activity: 50.7%
Activity: 50.7% Activity: 50.7% Activity: 50.7%
Last Achievements
Award-Showcase
NMR Credits: 0
NMR Points: 193,617
Downloads: 0
Uploads: 0
Default Hsqc - tocsy

HSQC - TOCSY

There are many different NMR techniques used to probe molecular structure. Two very useful methods are the HSQC (or HMQC) sequence used to obtain heteronuclear coupling correlations and the TOCSY sequence used to look at extended homonuclear coupling correlations. These are among the most widely used pulse sequences by chemists to help elucidate the molecular structure of organic molecules. The sequences can also be combined into a single HSQC-TOCSY sequence which is simply an HSQC followed by a TOCSY spin lock. For the case of 1H and 13C in organic molecules, the HSQC-TOCSY spectrum provides 1H TOCSY subspectra of the isolated spin systems in the rows at each of the 13C frequencies. Overlap of 1H resonances which may have appeared in a simple 1H TOCSY spectrum can be resolved in the HSQC-TOCSY spectrum provided there is enough shift dispersion in the 13C spectrum. An example of this is shown in the figure below. The top panel shows the HSQC-TOCSY spectrum for 3-heptanone collected on a 300 MHz instrument. One can see the two spin systems on either side of the carbonyl group, color coded in yellow and pink. The second panel shows an expansion of the region within the red square of the first panel. Here we can separate the overlapping quartet and triplet for the methylene protons on either side of the carbonyl group.



Source: University of Ottawa NMR Facility Blog
Reply With Quote


Did you find this post helpful? Yes | No

Reply
Similar Threads
Thread Thread Starter Forum Replies Last Post
[Question from NMRWiki Q&A forum] HSQC perturbation
HSQC perturbation Dear All, I am trying to determine the interaction between 2 proteins. Is there any reference or literature which report the determination of binding constant between 2 proteins in which intensity drop of HSQC cross peaks can be used as the measuring parameter. As when I titrate these 2 proteins only change which I observe is intensity drop. I tried other biophysical techniques to determine binding constant, but results are not satisfactory. Any suggestion would be useful Regards
nmrlearner News from other NMR forums 1 09-24-2013 02:18 AM
[NMRpipe Yahoo group] Re: N15 separated NOESY and TOCSY
Re: N15 separated NOESY and TOCSY Thank you for your replies. The processing script of TOCSY (NH plane): $NMRTXT/ext.xz.com fid/test%03d.fid xz001.fid nmrPipe -in xz001.fid \ ... -out xzf.ft2 More...
NMRpipe Yahoo group news News from other NMR forums 0 05-29-2011 11:00 AM
[NMRpipe Yahoo group] Re: N15 separated NOESY and TOCSY
Re: N15 separated NOESY and TOCSY Hi Iris, Another question is what do you mean by the NH vs HH planes in regards to the 3D matrix (a single 3D peak can't be positive viewed along one axis and More...
NMRpipe Yahoo group news News from other NMR forums 0 05-27-2011 10:40 AM
[NMRpipe Yahoo group] Re: N15 separated NOESY and TOCSY
Re: N15 separated NOESY and TOCSY Hi Iris, It's difficult to diagnose the problem without seeing the processing script, but what phase correction was used in the 15N dimension? If the initial More...
NMRpipe Yahoo group news News from other NMR forums 0 05-26-2011 10:34 PM
[NMRpipe Yahoo group] N15 separated NOESY and TOCSY
N15 separated NOESY and TOCSY Hello, I did a N15 separated TOCSY experiment and used the following script: #!/bin/csh bruk2pipe -in ./ser \ -bad 0.0 -aswap -DMX -decim 2632 -dspfvs 20 More...
NMRpipe Yahoo group news News from other NMR forums 0 05-26-2011 10:34 PM
[Question from NMRWiki Q&A forum] HCCH-Tocsy connectivities
HCCH-Tocsy connectivities What I see from this picture (linked from www.protein-nmr.org.uk): http://www.protein-nmr.org.uk/pictures/experiment_types/hcch_tocsy.png that shows an HCCH-TOCSY magnetisation transfer I do not see any transfer from HD to HB, but in my peaks I see a peak that seems it can be connected az Leu CB-HB2-HD22Can I have these peaks in HCCH-TOCSY? would you please introduce me a reference that tell me what kind of connection can be visible in HC(C)H-Tocsy
nmrlearner News from other NMR forums 0 11-17-2010 06:08 PM
[Nature network NMR forum] HCCH-TOCSY (0 replies)
HCCH-TOCSY (0 replies) Hello everybody, I am a biologist who has jumped into NMR :( I hope this is an active forum so that I can improve myself and can help someday to others. I have a very stupid question!!! Do you know if I can see some HN peaks correlated with C-beta in HCCH-TOCSY? Cheers,
nmrlearner News from other NMR forums 0 08-25-2010 02:58 AM
2D TOCSY Experiment
2D TOCSY Experiment More...
nmrlearner NMR bookmarks 0 08-19-2010 02:34 PM


Thread Tools Search this Thread
Search this Thread:

Advanced Search
Display Modes Rate This Thread
Rate This Thread:

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are Off
Pingbacks are Off
Refbacks are Off



BioNMR advertisements to pay for website hosting and domain registration. Nobody does it for us.



Powered by vBulletin® Version 3.7.3
Copyright ©2000 - 2018, Jelsoft Enterprises Ltd.
Copyright, BioNMR.com, 2003-2013
Search Engine Friendly URLs by vBSEO 3.6.0

All times are GMT. The time now is 06:22 AM.


Map