BioNMR
NMR aggregator & online community since 2003
BioNMR    
Learn or help to learn NMR - get free NMR books!
 

Go Back   BioNMR > NMR community > News from NMR blogs
Advanced Search



Jobs Groups Conferences Literature Pulse sequences Software forums Programs Sample preps Web resources BioNMR issues


Webservers
NMR processing:
MDD
NMR assignment:
Backbone:
Autoassign
MARS
UNIO Match
PINE
Side-chains:
UNIO ATNOS-Ascan
NOEs:
UNIO ATNOS-Candid
UNIO Candid
ASDP
Structure from NMR restraints:
Ab initio:
GeNMR
Cyana
XPLOR-NIH
ASDP
UNIO ATNOS-Candid
UNIO Candid
Fragment-based:
BMRB CS-Rosetta
Rosetta-NMR (Robetta)
Template-based:
GeNMR
I-TASSER
Refinement:
Amber
Structure from chemical shifts:
Fragment-based:
WeNMR CS-Rosetta
BMRB CS-Rosetta
Homology-based:
CS23D
Simshift
Torsion angles from chemical shifts:
Preditor
TALOS
Promega- Proline
Secondary structure from chemical shifts:
CSI (via RCI server)
TALOS
MICS caps, β-turns
d2D
PECAN
Flexibility from chemical shifts:
RCI
Interactions from chemical shifts:
HADDOCK
Chemical shifts re-referencing:
Shiftcor
UNIO Shiftinspector
LACS
CheckShift
RefDB
NMR model quality:
NOEs, other restraints:
PROSESS
PSVS
RPF scores
iCing
Chemical shifts:
PROSESS
CheShift2
Vasco
iCing
RDCs:
DC
Anisofit
Pseudocontact shifts:
Anisofit
Protein geomtery:
Resolution-by-Proxy
PROSESS
What-If
iCing
PSVS
MolProbity
SAVES2 or SAVES4
Vadar
Prosa
ProQ
MetaMQAPII
PSQS
Eval123D
STAN
Ramachandran Plot
Rampage
ERRAT
Verify_3D
Harmony
Quality Control Check
NMR spectrum prediction:
FANDAS
MestReS
V-NMR
Flexibility from structure:
Backbone S2
Methyl S2
B-factor
Molecular dynamics:
Gromacs
Amber
Antechamber
Chemical shifts prediction:
From structure:
Shiftx2
Sparta+
Camshift
CH3shift- Methyl
ArShift- Aromatic
ShiftS
Proshift
PPM
CheShift-2- Cα
From sequence:
Shifty
Camcoil
Poulsen_rc_CS
Disordered proteins:
MAXOCC
Format conversion & validation:
CCPN
From NMR-STAR 3.1
Validate NMR-STAR 3.1
NMR sample preparation:
Protein disorder:
DisMeta
Protein solubility:
camLILA
ccSOL
Camfold
camGroEL
Zyggregator
Isotope labeling:
UPLABEL
Solid-state NMR:
sedNMR


Reply
Thread Tools Search this Thread Rate Thread Display Modes
  #1  
Unread 07-25-2014, 06:55 PM
nmrlearner's Avatar
Senior Member
 
Join Date: Jan 2005
Posts: 23,134
Points: 193,617, Level: 100
Points: 193,617, Level: 100 Points: 193,617, Level: 100 Points: 193,617, Level: 100
Level up: 0%, 0 Points needed
Level up: 0% Level up: 0% Level up: 0%
Activity: 50.7%
Activity: 50.7% Activity: 50.7% Activity: 50.7%
Last Achievements
Award-Showcase
NMR Credits: 0
NMR Points: 193,617
Downloads: 0
Uploads: 0
Default Flux through hepatic pyruvate carboxylase and phosphoenolpyruvate carboxykinase detected by hyperpolarized 13C magnetic resonance

From The DNP-NMR Blog:

Flux through hepatic pyruvate carboxylase and phosphoenolpyruvate carboxykinase detected by hyperpolarized 13C magnetic resonance


Merritt, M.E., et al., Flux through hepatic pyruvate carboxylase and phosphoenolpyruvate carboxykinase detected by hyperpolarized 13C magnetic resonance. Proc. Nat. Aca. Sci. USA, 2011. 108(47): p. 19084-19089.


http://www.pnas.org/content/108/47/19084.abstract


In the heart, detection of hyperpolarized [13C]bicarbonate and 13CO2 by magnetic resonance (MR) after administration of hyperpolarized [1-13C]pyruvate is caused exclusively by oxidative decarboxylation of pyruvate via the pyruvate dehydrogenase complex (PDH). However, liver mitochondria possess alternative anabolic pathways accessible by [1-13C]pyruvate, which may allow a wider diagnostic range for hyperpolarized MR compared with other tissue. Metabolism of hyperpolarized [1-13C]pyruvate in the tricarboxylic acid (TCA) cycle was monitored in the isolated perfused liver from fed and fasted mice. Hyperpolarized [1-13C]pyruvate was rapidly converted to [1-13C]lactate, [1-13C]alanine, [1-13C]malate, [4-13C]malate, [1-13C]aspartate, [4-13C]aspartate, and [13C]bicarbonate. Livers from fasted animals had increased lactate:alanine, consistent with elevated NADH:NAD+. The appearance of asymmetrically enriched malate and aspartate indicated high rates of anaplerotic pyruvate carboxylase activity and incomplete equilibration with fumarate. Hyperpolarized [13C]bicarbonate was also detected, consistent with multiple mechanisms, including cataplerotic decarboxylation of [4-13C]oxaloacetate via phosphoenolpyruvate carboxykinase (PEPCK), forward TCA cycle flux of [4-13C]oxaloacetate to generate 13CO2 at isocitrate dehydrogenase, or decarboxylation of [1-13C]pyruvate by PDH. Isotopomer analysis of liver glutamate confirmed that anaplerosis was sevenfold greater than flux through PDH. In addition, signal from [4-13C]malate and [4-13C]aspartate was markedly blunted and signal from [13C]bicarbonate was completely abolished in livers from PEPCK KO mice, indicating that the major pathway for entry of hyperpolarized [1-13C]pyruvate into the hepatic TCA cycle is via pyruvate carboxylase, and that cataplerotic flux through PEPCK is the primary source of [13C]bicarbonate. We conclude that MR detection of hyperpolarized TCA intermediates and bicarbonate is diagnostic of pyruvate carboxylase and PEPCK flux in the liver.


Go to The DNP-NMR Blog for more info.
Reply With Quote


Did you find this post helpful? Yes | No

Reply
Similar Threads
Thread Thread Starter Forum Replies Last Post
Hyperpolarized singlet lifetimes of pyruvate in human blood and in the mouse
From The DNP-NMR Blog: Hyperpolarized singlet lifetimes of pyruvate in human blood and in the mouse Marco-Rius, I., et al., Hyperpolarized singlet lifetimes of pyruvate in human blood and in the mouse. NMR Biomed, 2013. 26(12): p. 1696-704. http://www.ncbi.nlm.nih.gov/pubmed/23946252
nmrlearner News from NMR blogs 0 03-19-2014 10:43 PM
Reproducibility study for free-breathing measurements of pyruvate metabolism using hyperpolarized 13C in the heart
From The DNP-NMR Blog: Reproducibility study for free-breathing measurements of pyruvate metabolism using hyperpolarized 13C in the heart Lau, A.Z., et al., Reproducibility study for free-breathing measurements of pyruvate metabolism using hyperpolarized (13) C in the heart. Magn Reson Med, 2013. 69(4): p. 1063-71. http://www.ncbi.nlm.nih.gov/pubmed/22760647
nmrlearner News from NMR blogs 0 03-17-2014 07:23 PM
In vivo magnetic resonance imaging of hyperpolarized silicon particles
From The DNP-NMR Blog: In vivo magnetic resonance imaging of hyperpolarized silicon particles Cassidy, M.C., et al., In vivo magnetic resonance imaging of hyperpolarized silicon particles. Nat Nano, 2013. 8(5): p. 363-368. http://dx.doi.org/10.1038/nnano.2013.65
nmrlearner News from NMR blogs 0 08-29-2013 01:27 AM
[NMR tweet] Hyperpolarized diamond nuclei increase NMR/MRI sensitivity: Today?s nuclear magnetic resonance (NMR) and magnetic... http://t.co/FJUKPENlLm
Hyperpolarized diamond nuclei increase NMR/MRI sensitivity: Today?s nuclear magnetic resonance (NMR) and magnetic... http://t.co/FJUKPENlLm Published by environmentguru (Environment Guru) on 2013-06-06T19:24:34Z Source: Twitter
nmrlearner Twitter NMR 0 06-07-2013 07:06 AM
Metabolic response of glioma to dichloroacetate measured in vivo by hyperpolarized 13C magnetic resonance spectroscopic imaging
From the The DNP-NMR Blog: Metabolic response of glioma to dichloroacetate measured in vivo by hyperpolarized 13C magnetic resonance spectroscopic imaging Park, J.M., et al., Metabolic response of glioma to dichloroacetate measured in vivo by hyperpolarized 13C magnetic resonance spectroscopic imaging. Neuro-Oncology, 2013. 15(4): p. 433-41. http://neuro-oncology.oxfordjournals.org/content/early/2013/01/16/neuonc.nos319.abstract
nmrlearner News from NMR blogs 0 04-15-2013 08:52 AM
Probing alanine transaminase catalysis with hyperpolarized 13CD3-pyruvate
From the The DNP-NMR Blog: Probing alanine transaminase catalysis with hyperpolarized 13CD3-pyruvate Barb, A.W., et al., Probing alanine transaminase catalysis with hyperpolarized 13CD3-pyruvate. J. Magn. Reson., 2013. 228(0): p. 59-65. http://dx.doi.org/10.1016/j.jmr.2012.12.013
nmrlearner News from NMR blogs 0 04-15-2013 08:52 AM
[NMR paper] 13C NMR evidence for pyruvate kinase flux attenuation underlying suppressed acid form
13C NMR evidence for pyruvate kinase flux attenuation underlying suppressed acid formation in Bacillus subtilis. Related Articles 13C NMR evidence for pyruvate kinase flux attenuation underlying suppressed acid formation in Bacillus subtilis. Biotechnol Prog. 2000 Mar-Apr;16(2):169-75 Authors: Phalakornkule C, Fry B, Zhu T, Kopesel R, Ataai MM, Domach MM When batch and continuous Bacillus subtilis cultures are provided with a small amount of citrate, acid production ceases, carbon yield increases by more than 2-fold, and the productivity of...
nmrlearner Journal club 0 11-18-2010 09:15 PM
[NMR paper] Enzyme IIIlac of the staphylococcal phosphoenolpyruvate-dependent phosphotransferase
Enzyme IIIlac of the staphylococcal phosphoenolpyruvate-dependent phosphotransferase system: site-specific mutagenesis of histidine residues, biochemical characterization and 1H-NMR studies. Related Articles Enzyme IIIlac of the staphylococcal phosphoenolpyruvate-dependent phosphotransferase system: site-specific mutagenesis of histidine residues, biochemical characterization and 1H-NMR studies. Protein Eng. 1991 Apr;4(4):469-73 Authors: Finkeldei U, Kalbitzer HR, Eisermann R, Stewart GC, Hengstenberg W The lactose-specific phosphocarrier...
nmrlearner Journal club 0 08-21-2010 11:16 PM


Thread Tools Search this Thread
Search this Thread:

Advanced Search
Display Modes Rate This Thread
Rate This Thread:

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are Off
Pingbacks are Off
Refbacks are Off



BioNMR advertisements to pay for website hosting and domain registration. Nobody does it for us.



Powered by vBulletin® Version 3.7.3
Copyright ©2000 - 2024, Jelsoft Enterprises Ltd.
Copyright, BioNMR.com, 2003-2013
Search Engine Friendly URLs by vBSEO 3.6.0

All times are GMT. The time now is 12:01 PM.


Map