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Default Efficient assignment and NMR analysis of an intact virus using sequential side-chain correlations and DNP sensitization #DNPNMR

From The DNP-NMR Blog:

Efficient assignment and NMR analysis of an intact virus using sequential side-chain correlations and DNP sensitization #DNPNMR

p.p1 {margin: 0.0px 0.0px 0.0px 36.0px; text-indent: -36.0px; font: 12.0px Helvetica}
Sergeyev, I.V., et al., Efficient assignment and NMR analysis of an intact virus using sequential side-chain correlations and DNP sensitization. Proc Natl Acad Sci U S A, 2017. 114(20): p. 5171-5176.


https://www.ncbi.nlm.nih.gov/pubmed/28461483


<div style="text-align: justify;">An experimental strategy has been developed to increase the efficiency of dynamic nuclear polarization (DNP) in solid-state NMR studies. The method makes assignments simpler, faster, and more reliable via sequential correlations of both side-chain and Calpha resonances. The approach is particularly suited to complex biomolecules and systems with significant chemical-shift degeneracy. It was designed to overcome the spectral congestion and line broadening that occur due to sample freezing at the cryogenic temperatures required for DNP. Nonuniform sampling (NUS) is incorporated to achieve time-efficient collection of multidimensional data. Additionally, fast (25 kHz) magic-angle spinning (MAS) provides optimal sensitivity and resolution. Data collected in
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