Sauer, G., et al., Effective PHIP labeling of bioactive peptides boosts the intensity of the NMR signal. Angew Chem Int Ed Engl, 2014. 53(47): p. 12941-5.
A series of novel bioactive derivatives of the sunflower trypsin inhibitor-1 (SFTI-1) suitable for hyperpolarization by parahydrogen-induced polarization (PHIP) was developed. The PHIP activity was achieved by labeling with L-propargylglycine, O-propargyl-L-tyrosine, or 4-pentynoic acid. (1) H NMR signal enhancements (SE) of up to a factor of 70 were achieved in aqueous solution. We found that an isolated spatial location of the triple bond within the respective label and its accessibility for the hydrogenation catalyst are essential factors for the degree of signal enhancement.
[Question from NMRWiki Q&A forum] PHIP experiments
PHIP experiments
Dear all! I am struggling with some density matrices in PHIP experiments. I understand why the density matrix before hydrogenation reaction is I dot S But why afterwards (in PASADENA exp. for example) the density matrix is just IzSz? And in ALTADENA is IzSz-0.5(Iz-Sz)? Thank you!
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nmrlearner
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11-28-2013 05:18 PM
Protein that boosts longevity may protect against diabetes - R & D Magazine
Protein that boosts longevity may protect against diabetes - R & D Magazine
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Protein that boosts longevity may protect against diabetes
R & D Magazine
A protein that slows aging in mice and other animals also protects against the ravages of a high-fat diet, including diabetes, according to a new MIT study. MIT biology professor ... JEOL to launch world's smallest solid-state NMR probe. Apr 12 ...
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nmrlearner
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08-09-2012 08:10 AM
Postdoc NMR - PHIP
Postdoc NMR - PHIP
Currently we are looking for a post-doctoral researcher in the area of para-hydrogen induced hyperpolarisation (PHIP). The position aims specifically for development of the PHIP methodology and instrumentation for screening, biomarker research and/or molecular diagnostics. The post-doc (initially 2 yrs; extension is possible) should have a background in (Physical) Chemistry and/or Analytical Sciences with knowledge of MR spectroscopy (e.g. NMR or MRI) and an interest in methodological development and instrumentation. Expertise in para-hydrogen based NMR technology is an...
Effective PHIP labeling of bioactive peptides boosts the intensity of the NMR signal
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