Cheng, C.Y. and S. Han, Dynamic nuclear polarization methods in solids and solutions to explore membrane proteins and membrane systems. Annu Rev Phys Chem, 2013. 64(1): p. 507-32.
Membrane proteins regulate vital cellular processes, including signaling, ion transport, and vesicular trafficking. Obtaining experimental access to their structures, conformational fluctuations, orientations, locations, and hydration in membrane environments, as well as the lipid membrane properties, is critical to understanding their functions. Dynamic nuclear polarization (DNP) of frozen solids can dramatically boost the sensitivity of current solid-state nuclear magnetic resonance tools to enhance access to membrane protein structures in native membrane environments. Overhauser DNP in the solution state can map out the local and site-specific hydration dynamics landscape of membrane proteins and lipid membranes, critically complementing the structural and dynamics information obtained by electron paramagnetic resonance spectroscopy. Here, we provide an overview of how DNP methods in solids and solutions can significantly increase our understanding of membrane protein structures, dynamics, functions, and hydration in complex biological membrane environments.
[NMR paper] Structure and Dynamic Properties of Membrane Proteins using NMR.
Structure and Dynamic Properties of Membrane Proteins using NMR.
Structure and Dynamic Properties of Membrane Proteins using NMR.
Compr Physiol. 2012 Apr 1;2(2):1491-1539
Authors: Rösner HI, Kragelund BB
Abstract
Integral membrane proteins are one of the most challenging groups of macromolecules despite their apparent conformational simplicity. They manage and drive transport, circulate information, and participate in cellular movements via interactions with other proteins and through intricate conformational changes. Their...
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06-27-2013 02:10 PM
Dynamic nuclear polarization-enhanced 13C NMR spectroscopy of static biological solids
From The DNP-NMR Blog:
Dynamic nuclear polarization-enhanced 13C NMR spectroscopy of static biological solids
Potapov, A., W.M. Yau, and R. Tycko, Dynamic nuclear polarization-enhanced (13)C NMR spectroscopy of static biological solids. J Magn Reson, 2013. 231(0): p. 5-14.
http://www.ncbi.nlm.nih.gov/pubmed/23562665
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06-25-2013 12:17 AM
TEMPOL as a polarizing agent for dynamic nuclear polarization of aqueous solutions
From The DNP-NMR Blog:
TEMPOL as a polarizing agent for dynamic nuclear polarization of aqueous solutions
Gafurov, M., TEMPOL as a polarizing agent for dynamic nuclear polarization of aqueous solutions. Magn. Reson. Solids., 2013. 15: p. 13103.
http://mrsej.ksu.ru/contents.html#13103
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05-03-2013 02:26 PM
Dynamic nuclear polarization-enhanced 13C NMR spectroscopy of static biological solids
Dynamic nuclear polarization-enhanced 13C NMR spectroscopy of static biological solids
Available online 27 February 2013
Publication year: 2013
Source:Journal of Magnetic Resonance</br>
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We explore the possibility of using dynamic nuclear polarization (DNP) to enhance signals in structural studies of biological solids by solid state NMR without sample spinning. Specifically, we use 2D 13C-13C exchange spectroscopy to probe the peptide backbone torsion angles (?,?) in a series of selectively 13C-labeled 40-residue ?-amyloid (A ?1-40) samples, in both fibrillar and...
Fast passage dynamic nuclear polarization on rotating solids
Fast passage dynamic nuclear polarization on rotating solids
November 2012
Publication year: 2012
Source:Journal of Magnetic Resonance, Volume 224</br>
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Magic Angle Spinning (MAS) Dynamic Nuclear Polarization (DNP) has proven to be a very powerful way to improve the signal to noise ratio of NMR experiments on solids. The experiments have in general been interpreted considering the Solid-Effect (SE) and Cross-Effect (CE) DNP mechanisms while ignoring the influence of sample spinning. In this paper, we show experimental data of MAS-DNP enhancements of 1H and 13C in...
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12-01-2012 06:10 PM
Fast Passage Dynamic Nuclear Polarization on Rotating Solids
Fast Passage Dynamic Nuclear Polarization on Rotating Solids
Publication year: 2012
Source:Journal of Magnetic Resonance</br>
Frederic Mentink-Vigier, Ümit Akbey, Yonatan Hovav, Shimon Vega, Hartmut Oschkinat, Akiva Feintuch</br>
Magic Angle Spinning (MAS) Dynamic Nuclear Polarization (DNP) has proven to be a very powerful way to improve the signal to noise ratio of NMR experiments on solids. The experiments have in general been interpreted considering the Solid-Effect (SE) and Cross-Effect (CE) DNP mechanisms while ignoring the influence of sample spinning. In this...
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08-25-2012 08:56 AM
[NMR paper] Dynamic pictures of membrane proteins in two-dimensional crystal, lipid bilayer and d
Dynamic pictures of membrane proteins in two-dimensional crystal, lipid bilayer and detergent as revealed by site-directed solid-state 13C NMR.
Related Articles Dynamic pictures of membrane proteins in two-dimensional crystal, lipid bilayer and detergent as revealed by site-directed solid-state 13C NMR.
Chem Phys Lipids. 2004 Nov;132(1):101-12
Authors: Saitō H
We have compared site-directed 13C solid-state NMR spectra of Ala- and/or Val-labeled membrane proteins, including bacteriorhodopsin (bR), pharaonis phoborhodopin (ppR), its cognate...
Dynamic nuclear polarization methods in solids and solutions to explore membrane proteins and membrane systems
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