This study aims to investigate the feasibility of dynamic hyperpolarized 13C MR spectroscopic imaging (MRSI) using the SPectroscopic Imaging by exploiting spatiospectral CorrElation (SPICE) technique and an estimation of the spatially resolved conversion constant rate (kpl). An acquisition scheme comprising a single training dataset and several imaging datasets was proposed considering hyperpolarized 13C circumstances. The feasibility and advantage of the scheme were investigated in two parts: (a) consistency of spectral basis over time and (b) accuracy of the estimated kpl. The simulations and in vivo experiments support accurate kpl estimation with consistent spectral bases. The proposed method was implemented in an enzyme phantom and via in vivo experiments. In the enzyme phantom experiments, spatially resolved homogeneous kpl maps were observed. In the in vivo experiments, normal diet (ND) mice and high-fat diet (HFD) mice had kpl (s-1) values of medullar (ND: 0.0119 ± 0.0022, HFD: 0.0195 ± 0.0005) and cortical (ND: 0.0148 ±0.0023, HFD: 0.0224 ±0.0054) regions which were higher than vascular (ND: 0.0087 ±0.0013, HFD: 0.0132 ±0.0050) regions. In particular, the kpl value in the medullar region exhibited a significant difference between the two diet groups. In summary, the feasibility of using modified SPICE for dynamic hyperpolarized 13C MRSI was demonstrated via simulations and in vivo experiments. The consistency of spectral bases over time and the accuracy of the estimated kpl values validate the proposed acquisition scheme, which comprises only a single training dataset. The proposed method improved the spatial resolution of dynamic hyperpolarized 13C MRSI, which could be used for kpl estimation using high signal-to-noise ratio spectral bases.
Free Radical Imaging Using In Vivo Dynamic Nuclear Polarization-MRI #DNPNMR #ODNP
From The DNP-NMR Blog:
Free Radical Imaging Using In Vivo Dynamic Nuclear Polarization-MRI #DNPNMR #ODNP
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Utsumi, H. and F. Hyodo, Free Radical Imaging Using In Vivo Dynamic Nuclear Polarization-MRI. Methods Enzymol, 2015. 564: p. 553-71.
https://www.ncbi.nlm.nih.gov/pubmed/26477265
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08-18-2017 04:59 PM
Simultaneous and spectroscopic redox molecular imaging of multiple free radical intermediates using dynamic nuclear polarization-magnetic resonance imaging #DNPNMR
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Simultaneous and spectroscopic redox molecular imaging of multiple free radical intermediates using dynamic nuclear polarization-magnetic resonance imaging #DNPNMR
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Hyodo, F., et al., Simultaneous and spectroscopic redox molecular imaging of multiple free radical intermediates using dynamic nuclear polarization-magnetic resonance imaging. Anal Chem, 2014. 86(15): p. 7234-8.
https://www.ncbi.nlm.nih.gov/pubmed/25036767
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08-17-2017 12:56 AM
Dynamic nuclear polarization of biocompatible (13)C-enriched carbonates for in vivo pH imaging #DNPNMR
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Dynamic nuclear polarization of biocompatible (13)C-enriched carbonates for in vivo pH imaging #DNPNMR
Korenchan, D.E., et al., Dynamic nuclear polarization of biocompatible (13)C-enriched carbonates for in vivo pH imaging. Chem Commun (Camb), 2016. 52(14): p. 3030-3.
http://www.ncbi.nlm.nih.gov/pubmed/26792559
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06-02-2016 02:11 AM
In vivo single-shot 13C spectroscopic imaging of hyperpolarized metabolites by spatiotemporal encoding
From The DNP-NMR Blog:
In vivo single-shot 13C spectroscopic imaging of hyperpolarized metabolites by spatiotemporal encoding
Schmidt, R., et al., In vivo single-shot 13C spectroscopic imaging of hyperpolarized metabolites by spatiotemporal encoding. J Magn Reson, 2014. 240(0): p. 8-15.
http://www.ncbi.nlm.nih.gov/pubmed/24486720
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06-02-2014 10:02 PM
In vivo single-shot C spectroscopic imaging of hyperpolarized metabolites by spatiotemporal encoding
From The DNP-NMR Blog:
In vivo single-shot C spectroscopic imaging of hyperpolarized metabolites by spatiotemporal encoding
Schmidt, R., et al., In vivo single-shot C spectroscopic imaging of hyperpolarized metabolites by spatiotemporal encoding. J Magn Reson, 2014. 240C(0): p. 8-15.
http://www.ncbi.nlm.nih.gov/pubmed/24486720
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02-19-2014 03:12 PM
Strategies for rapid in vivo 1H and hyperpolarized 13C MR spectroscopic imaging
From the The DNP-NMR Blog:
Strategies for rapid in vivo 1H and hyperpolarized 13C MR spectroscopic imaging
Nelson, S.J., et al., Strategies for rapid in vivo 1H and hyperpolarized 13C MR spectroscopic imaging. J. Magn. Reson., 2013. 229(0): p. 187-197.
http://dx.doi.org/10.1016/j.jmr.2013.02.003
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04-15-2013 08:52 AM
Metabolic response of glioma to dichloroacetate measured in vivo by hyperpolarized 13C magnetic resonance spectroscopic imaging
From the The DNP-NMR Blog:
Metabolic response of glioma to dichloroacetate measured in vivo by hyperpolarized 13C magnetic resonance spectroscopic imaging
Park, J.M., et al., Metabolic response of glioma to dichloroacetate measured in vivo by hyperpolarized 13C magnetic resonance spectroscopic imaging. Neuro-Oncology, 2013. 15(4): p. 433-41.
http://neuro-oncology.oxfordjournals.org/content/early/2013/01/16/neuonc.nos319.abstract
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04-15-2013 08:52 AM
Detection of autosomal dominant polycystic kidney disease by NMR spectroscopic fingerprinting of urine.
Detection of autosomal dominant polycystic kidney disease by NMR spectroscopic fingerprinting of urine.
Detection of autosomal dominant polycystic kidney disease by NMR spectroscopic fingerprinting of urine.
Kidney Int. 2011 Mar 9;
Authors: Gronwald W, Klein MS, Zeltner R, Schulze BD, Reinhold SW, Deutschmann M, Immervoll AK, Böger CA, Banas B, Eckardt KU, Oefner PJ
Autosomal dominant polycystic kidney disease (ADPKD) is a frequent cause of kidney failure; however, urinary biomarkers for the disease are lacking. In a step towards identifying such...
Dynamic hyperpolarized 13C MR spectroscopic imaging using SPICE in mouse kidney at 9.4 T #DNPNMR
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