Hyperpolarization techniques, such as Overhauser dynamic nuclear polarization (DNP), can provide a dramatic increase in the signal obtained from nuclear magnetic resonance experiments and may therefore enable new applications where sensitivity is a limiting factor. In this contribution, studies of the (1)H and (19)F Overhauser dynamic nuclear polarization enhancements at 345 mT are presented for three different aromatic solvents with the TEMPO radical for a range of radical concentrations. Furthermore, nuclear magnetic relaxation dispersion measurements of the same solutions are analyzed, showing contributions from dipolar and scalar coupling modulated by translational diffusion and different coupling efficiency for different solvents and nuclei. Measurements of the electron paramagnetic resonance linewidth are included to support the analysis of the DNP saturation factor for varying radical concentration. The results of our study give an insight into the characteristics of nitroxide radicals as polarizing agents for (19)F Overhauser DNP of aromatic fluorinated solvents. Furthermore, we compare our results with the findings of the extensive research on Overhauser DNP that was conducted in the past for a large variety of other radicals.
[NMR paper] A Comparative CEST NMR Study of Slow Conformational Dynamics of Small GTPases Complexed with GTP and GTP Analogues.
A Comparative CEST NMR Study of Slow Conformational Dynamics of Small GTPases Complexed with GTP and GTP Analogues.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--media.wiley.com-assets-2250-98-WileyOnlineLibrary-Button_120x27px_FullText.gif Related Articles A Comparative CEST NMR Study of Slow Conformational Dynamics of Small GTPases Complexed with GTP and GTP Analogues.
Angew Chem Int Ed Engl. 2013 Aug 22;
Authors: Long D, Marshall CB, Bouvignies G, Mazhab-Jafari MT, Smith MJ, Ikura M, Kay LE
Abstract
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[NMR paper] A 1H NMR comparative study of the structure of the critical packing interfaces betwee
A 1H NMR comparative study of the structure of the critical packing interfaces between helix and non-helical region in various ligation states of sperm whale myoglobin.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles A 1H NMR comparative study of the structure of the critical packing interfaces between helix and non-helical region in various ligation states of sperm whale myoglobin.
Biochim Biophys Acta. 1997 Nov 14;1343(1):59-66
Authors: Yamamoto Y
NMR signals arising...
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[NMR paper] Transferred nuclear Overhauser effect study of the C-terminal helix of yeast phosphog
Transferred nuclear Overhauser effect study of the C-terminal helix of yeast phosphoglycerate kinase: NMR solution structure of the C-terminal bound peptide.
Related Articles Transferred nuclear Overhauser effect study of the C-terminal helix of yeast phosphoglycerate kinase: NMR solution structure of the C-terminal bound peptide.
Biochemistry. 1995 Jan 24;34(3):842-6
Authors: Andrieux M, Leroy E, Guittet E, Ritco-Vonsovici M, Mouratou B, Minard P, Desmadril M, Yon JM
Two-dimensional 1H nuclear magnetic resonance spectroscopy is used to...
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[NMR paper] 1H-NMR comparative study of the active site in shark (Galeorhinus japonicus), horse,
1H-NMR comparative study of the active site in shark (Galeorhinus japonicus), horse, and sperm whale deoxy myoglobins.
Related Articles 1H-NMR comparative study of the active site in shark (Galeorhinus japonicus), horse, and sperm whale deoxy myoglobins.
J Biochem. 1992 Sep;112(3):414-20
Authors: Yamamoto Y, Iwafune K, Chûjô R, Inoue Y, Imai K, Suzuki T
1H-NMR spectra of deoxy myoglobins (Mbs) from shark (Galeorhinus japonicus), horse, and sperm whale have been studied to gain insights into their active site structure. It has been...
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[NMR paper] A 31P-NMR spin-lattice relaxation and 31P[1H] nuclear Overhauser effect study of soni
A 31P-NMR spin-lattice relaxation and 31P nuclear Overhauser effect study of sonicated small unilamellar phosphatidylcholine vesicles.
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Biochim Biophys Acta. 1992 Feb 17;1104(1):137-46
Authors: Tauskela JS, Thompson M
The motional properties of the inner and outer monolayer headgroups of...
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[NMR paper] The use of 19F NMR in the study of protein alkylation by fluorinated reactive interme
The use of 19F NMR in the study of protein alkylation by fluorinated reactive intermediates.
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Adv Exp Med Biol. 1991;283:735-8
Authors: Harris JW, Anders MW
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[NMR paper] NMR studies of fluorinated visual pigment analogs.
NMR studies of fluorinated visual pigment analogs.
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Biochem Biophys Res Commun. 1991 Sep 30;179(3):1337-43
Authors: Colmenares LU, Asato AE, Denny M, Mead D, Zingoni JP, Liu RS
The 19F-nmr chemical shift data of isomeric pigments (11-cis and 9-cis) of four vinyl fluororhodopsins and two trifluororhodopsins have been recorded. When compared with model protonated Schiff...
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[NMR paper] Proton NMR study of the comparative electronic/magnetic properties and dynamics of th
Proton NMR study of the comparative electronic/magnetic properties and dynamics of the acid in equilibrium with alkaline transition in a series of ferricytochromes c'.
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J Biol Chem. 1990 Sep 25;265(27):16173-80
Authors: La Mar GN, Jackson JT, Dugad LB, Cusanovich MA, Bartsch RG
The proton NMR spectra of ferricytochrome c' from Rhodopseudomonas palustris,...
A comparative study of (1)H and (19)F Overhauser DNP in fluorinated benzenes
Linear Mode
