The bigger they are, the harder they fall: A topical review on sedimented solutes for solid-state NMR

		BioNMR > NMR community > News from NMR blogs
The bigger they are, the harder they fall: A topical review on sedimented solutes for solid-state NMR

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

View First Unread

Thread Tools

Search this Thread

Rate Thread

Display Modes

07-31-2015, 07:53 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,174

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

The bigger they are, the harder they fall: A topical review on sedimented solutes for solid-state NMR

From The DNP-NMR Blog:

The bigger they are, the harder they fall: A topical review on sedimented solutes for solid-state NMR

Preparing samples for DNP experiments sometimes involves using sedimented solutes. This is a nice overview of the technique and how it is beneficial for DNP-NMR spectroscopy.

Ravera, E., The bigger they are, the harder they fall: A topical review on sedimented solutes for solid-state NMR. Concepts in Magnetic Resonance Part A, 2014. 43(6): p. 209-227.

http://dx.doi.org/10.1002/cmr.a.21318

Sample preparation for solid-state NMR of soluble proteins is all but a trivial accomplishment and often represents the factor that determines the success of the experiments, despite the dramatic methodological and technological improvements in the field of SSNMR. Several different sample preparation have been proposed and applied over the years, with varied results. Among those methods we have proposed the use of sedimentation for obtaining samples with high sensitivity and resolution. This review aims at covering the applications of NMR to sedimented solutes from its first description to the latest developments with implications in the field of DNP. © 2015 Wiley Periodicals, Inc. Concepts Magn Reson Part A 43A: 209–227, 2015.

Go to The DNP-NMR Blog for more info.

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
[NMR paper] Solid-state NMR of the Yersinia pestis outer membrane protein Ail in lipid bilayer nanodiscs sedimented by ultracentrifugation. Solid-state NMR of the Yersinia pestis outer membrane protein Ail in lipid bilayer nanodiscs sedimented by ultracentrifugation. Solid-state NMR of the Yersinia pestis outer membrane protein Ail in lipid bilayer nanodiscs sedimented by ultracentrifugation. J Biomol NMR. 2015 Jan 13; Authors: Ding Y, Fujimoto LM, Yao Y, Marassi FM Abstract Solid-state NMR studies of sedimented soluble proteins has been developed recently as an attractive approach for overcoming the size limitations of solution NMR spectroscopy while bypassing the...	nmrlearner	Journal club	0	01-13-2015 02:31 PM
Solid-state NMR of the Yersinia pestis outer membrane protein Ail in lipid bilayer nanodiscs sedimented by ultracentrifugation Solid-state NMR of the Yersinia pestis outer membrane protein Ail in lipid bilayer nanodiscs sedimented by ultracentrifugation Abstract Solid-state NMR studies of sedimented soluble proteins has been developed recently as an attractive approach for overcoming the size limitations of solution NMR spectroscopy while bypassing the need for sample crystallization or precipitation (Bertini et al. Proc Natl Acad Sci USA 108(26):10396â??10399, 2011). Inspired by the potential benefits of this method, we have investigated the ability to sediment lipid bilayer...	nmrlearner	Journal club	0	01-12-2015 11:31 PM
[Question from NMRWiki Q&A forum] bigger MAS-NMR spectrum as irradiation window bigger MAS-NMR spectrum as irradiation window Hi, I have a sample with a very very big CSA which is broader than my irradiation windows, so I have to acquire my data in 2 or more experiments (shaped pulses are enough and I can't go for another probehead). I need to do a simulation of the resulting spectrum, but I have to make sure my irradiation power is the same everywhere so the resulting sum spectrum isn't distorted. I heard about an article about how to choose the different irradiation windows of each measure so the sum still can be simulated but I couldn't get the source. I...	nmrlearner	News from other NMR forums	0	12-17-2014 08:03 AM
Dynamic Nuclear Polarization of Sedimented Solutes From the The DNP-NMR Blog: Dynamic Nuclear Polarization of Sedimented Solutes Ravera, E., et al., Dynamic Nuclear Polarization of Sedimented Solutes. J. Am. Chem. Soc., 2013. 135(5): p. 1641-1644. http://dx.doi.org/10.1021/ja312553b	nmrlearner	News from NMR blogs	0	04-15-2013 08:52 AM
Solid-state NMR of proteins sedimented by ultracentrifugation [Chemistry] Solid-state NMR of proteins sedimented by ultracentrifugation Bertini, I., Luchinat, C., Parigi, G., Ravera, E., Reif, B., Turano, P.... Date: 2011-06-28 Relatively large proteins in solution, spun in NMR rotors for solid samples at typical ultracentrifugation speeds, sediment at the rotor wall. The sedimented proteins provide high-quality solid-state-like NMR spectra suitable for structural investigation. The proteins fully revert to the native solution state when spinning is stopped, allowing one to study them in both conditions. Transiently sedimented proteins can be considered a...	nmrlearner	Journal club	0	06-29-2011 04:45 AM
Solid-state NMR of proteins sedimented by ultracentrifugation. Solid-state NMR of proteins sedimented by ultracentrifugation. Solid-state NMR of proteins sedimented by ultracentrifugation. Proc Natl Acad Sci U S A. 2011 Jun 13; Authors: Bertini I, Luchinat C, Parigi G, Ravera E, Reif B, Turano P Relatively large proteins in solution, spun in NMR rotors for solid samples at typical ultracentrifugation speeds, sediment at the rotor wall. The sedimented proteins provide high-quality solid-state-like NMR spectra suitable for structural investigation. The proteins fully revert to the native solution state when...	nmrlearner	Journal club	0	06-15-2011 01:15 PM
[NMR900 blog] 2010 MRS Fall Meeting 2010 MRS Fall Meeting November 29 - December 3, 2010, Boston, MA Abstracts for technical symposia are available online http://www.mrs.org/s_mrs/sec.asp?CID=27791&DID=332879 https://blogger.googleusercontent.com/tracker/8663203727601106205-4133507382418019445?l=nmr900.blogspot.com Read complete story on NMR900 blog	nmrlearner	News from NMR blogs	0	11-06-2010 01:08 AM
Solution- and solid-state NMR studies of GPCRs and their ligands (Review). Solution- and solid-state NMR studies of GPCRs and their ligands (Review). Related Articles Solution- and solid-state NMR studies of GPCRs and their ligands (Review). Biochim Biophys Acta. 2010 Oct 14; Authors: Tapaneeyakorn S, Goddard AD, Oates J, Willis CL, Watts A G protein-coupled receptors (GPCRs) represent one of the major targets of new drugs on the market given their roles as key membrane receptors in many cellular signalling pathways. Structure-based drug design has potential to be the most reliable method for novel drug discovery....	nmrlearner	Journal club	0	10-19-2010 04:51 PM

« Previous Thread | Next Thread »

Thread Tools	Search this Thread
Show Printable Version Email this Page	Search this Thread: Advanced Search
Display Modes	Rate This Thread
Linear Mode Switch to Hybrid Mode Switch to Threaded Mode	Rate This Thread:

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is Off Trackbacks are Off Pingbacks are Off Refbacks are Off