BioNMR
NMR aggregator & online community since 2003
BioNMR    
Learn or help to learn NMR - get free NMR books!
 

Go Back   BioNMR > NMR community > News from NMR blogs
Advanced Search



Jobs Groups Conferences Literature Pulse sequences Software forums Programs Sample preps Web resources BioNMR issues


Webservers
NMR processing:
MDD
NMR assignment:
Backbone:
Autoassign
MARS
UNIO Match
PINE
Side-chains:
UNIO ATNOS-Ascan
NOEs:
UNIO ATNOS-Candid
UNIO Candid
ASDP
Structure from NMR restraints:
Ab initio:
GeNMR
Cyana
XPLOR-NIH
ASDP
UNIO ATNOS-Candid
UNIO Candid
Fragment-based:
BMRB CS-Rosetta
Rosetta-NMR (Robetta)
Template-based:
GeNMR
I-TASSER
Refinement:
Amber
Structure from chemical shifts:
Fragment-based:
WeNMR CS-Rosetta
BMRB CS-Rosetta
Homology-based:
CS23D
Simshift
Torsion angles from chemical shifts:
Preditor
TALOS
Promega- Proline
Secondary structure from chemical shifts:
CSI (via RCI server)
TALOS
MICS caps, β-turns
d2D
PECAN
Flexibility from chemical shifts:
RCI
Interactions from chemical shifts:
HADDOCK
Chemical shifts re-referencing:
Shiftcor
UNIO Shiftinspector
LACS
CheckShift
RefDB
NMR model quality:
NOEs, other restraints:
PROSESS
PSVS
RPF scores
iCing
Chemical shifts:
PROSESS
CheShift2
Vasco
iCing
RDCs:
DC
Anisofit
Pseudocontact shifts:
Anisofit
Protein geomtery:
Resolution-by-Proxy
PROSESS
What-If
iCing
PSVS
MolProbity
SAVES2 or SAVES4
Vadar
Prosa
ProQ
MetaMQAPII
PSQS
Eval123D
STAN
Ramachandran Plot
Rampage
ERRAT
Verify_3D
Harmony
Quality Control Check
NMR spectrum prediction:
FANDAS
MestReS
V-NMR
Flexibility from structure:
Backbone S2
Methyl S2
B-factor
Molecular dynamics:
Gromacs
Amber
Antechamber
Chemical shifts prediction:
From structure:
Shiftx2
Sparta+
Camshift
CH3shift- Methyl
ArShift- Aromatic
ShiftS
Proshift
PPM
CheShift-2- Cα
From sequence:
Shifty
Camcoil
Poulsen_rc_CS
Disordered proteins:
MAXOCC
Format conversion & validation:
CCPN
From NMR-STAR 3.1
Validate NMR-STAR 3.1
NMR sample preparation:
Protein disorder:
DisMeta
Protein solubility:
camLILA
ccSOL
Camfold
camGroEL
Zyggregator
Isotope labeling:
UPLABEL
Solid-state NMR:
sedNMR


Reply
Thread Tools Search this Thread Rate Thread Display Modes
  #1  
Unread 01-03-2017, 01:47 AM
nmrlearner's Avatar
Senior Member
 
Join Date: Jan 2005
Posts: 23,135
Points: 193,617, Level: 100
Points: 193,617, Level: 100 Points: 193,617, Level: 100 Points: 193,617, Level: 100
Level up: 0%, 0 Points needed
Level up: 0% Level up: 0% Level up: 0%
Activity: 50.7%
Activity: 50.7% Activity: 50.7% Activity: 50.7%
Last Achievements
Award-Showcase
NMR Credits: 0
NMR Points: 193,617
Downloads: 0
Uploads: 0
Default Antioxidant treatment attenuates lactate production in diabetic nephropathy

From The DNP-NMR Blog:

Antioxidant treatment attenuates lactate production in diabetic nephropathy

p.p1 {margin: 0.0px 0.0px 0.0px 0.0px; font: 12.0px Helvetica}
p.p1 {margin: 0.0px 0.0px 0.0px 36.0px; text-indent: -36.0px; font: 12.0px Helvetica}
Laustsen, C., et al., Antioxidant treatment attenuates lactate production in diabetic nephropathy. American Journal of Physiology - Renal Physiology, 2016: p. ajprenal.00148.2016.


http://ajprenal.physiology.org/conte....2016.full.pdf


The early progression of diabetic nephropathy is notoriously difficult to detect and quantify prior to the occurrence of substantial histological damage. Recently, hyperpolarized [1-13C] pyruvate has demonstrated increased lactate production in the kidney early after the onset of diabetes, implying increased lactate dehydrogenase activity as a consequence of increased nicotinamide adenine dinucleotide substrate availability due to upregulation of the polyol pathway, i.e., pseudohypoxia. In this study, we investigated the role of oxidative stress in mediating these metabolic alterations using state-of-the-art hyperpolarized magnetic resonance (MR) imaging. Ten-week-old female Wistar rats were randomly divided into three groups: healthy controls, untreated diabetic (streptozotocin treatment to induce insulinopenic diabetes), and diabetic, receiving chronic antioxidant treatment with TEMPOL (4-hydroxy-2,2,6,6-tetramethylpiperidin-1-oxyl) via the drinking water. Examinations were performed 2, 3, and 4 weeks after the induction of diabetes by using a 3T Clinical MR system equipped with a dual tuned 13C/1H-volume rat coil. The rats received intravenous hyperpolarized [1-13C] pyruvate and were imaged using a slice-selective 13C-IDEAL spiral sequence. Untreated diabetic rats showed increased renal lactate production compared to that shown by the controls. However, chronic TEMPOL treatment significantly attenuated diabetes-induced lactate production. No significant effects of diabetes or TEMPOL were observed on 13C-alanine levels, indicating an intact glucose-alanine cycle, or 13C-bicarbonate, indicating normal flux through the Krebs cycle. In conclusion, this study demonstrates that diabetes-induced pseudohypoxia, as indicated by an increased lactate-to-pyruvate ratio, is significantly attenuated by antioxidant treatment. This demonstrates a pivotal role of oxidative stress for renal metabolic alterations occurring in early diabetes.
p.p1 {margin: 0.0px 0.0px 0.0px 0.0px; font: 12.0px Helvetica}

Go to The DNP-NMR Blog for more info.
Reply With Quote


Did you find this post helpful? Yes | No

Reply
Similar Threads
Thread Thread Starter Forum Replies Last Post
Production of hyperpolarized [1,4-13C2]malate from [1,4-13C2]fumarate is a marker of cell necrosis and treatment response in tumors
From The DNP-NMR Blog: Production of hyperpolarized malate from fumarate is a marker of cell necrosis and treatment response in tumors Gallagher, F.A., M.I. Kettunen, D.E. Hu, P.R. Jensen, R.I. Zandt, M. Karlsson, A. Gisselsson, S.K. Nelson, T.H. Witney, S.E. Bohndiek, G. Hansson, T. Peitersen, M.H. Lerche, and K.M. Brindle, Proc Natl Acad Sci U S A, 106, (2009) http://www.ncbi.nlm.nih.gov/pubmed/19903889
nmrlearner News from NMR blogs 0 12-16-2015 10:32 PM
The antioxidant role of proteins containing selenium - Medical Xpress
http://www.bionmr.com//t3.gstatic.com/images?q=tbn:ANd9GcSFmjKJueH8sAToPwf6NTRqSMgpmXF5eN5IlNWIrF-aWjIE0gtwFZ4ekXGsw23ht2fak3YoJkNr Medical Xpress <img alt="" height="1" width="1" /> The antioxidant role of proteins containing selenium Medical Xpress Rozovsky and her team used mass spectrometry and nuclear magnetic resonance spectroscopy to study selenium in redox biology, which the journal called a new approach that deepens scientific understanding of the chemical properties involved. Rozovsky ... The antioxidant role of proteins containing selenium - Medical Xpress
nmrlearner Online News 0 04-28-2014 10:34 PM
Journal Highlight: 1H and 13C NMR-based sugar profiling with chemometric analysis and antioxidant activity of herbhoneys and honeys
Journal Highlight: 1H and 13C NMR-based sugar profiling with chemometric analysis and antioxidant activity of herbhoneys and honeys http://www.spectroscopynow.com/common/images/thumbnails/1447e6dff3b.jpg1H and 13C NMR spectroscopy coupled with chemometric analysis (PCA and PLS-DA) and antioxidant assays were used to study 25 samples of Polish herbhoneys and honeys. Read the rest at Spectroscopynow.com
nmrlearner General 0 03-03-2014 04:55 PM
[NMR paper] Futile cycling of lactate through the plasma membrane of C6 glioma cells as detected
Futile cycling of lactate through the plasma membrane of C6 glioma cells as detected by (13C, 2H) NMR. Related Articles Futile cycling of lactate through the plasma membrane of C6 glioma cells as detected by (13C, 2H) NMR. J Neurosci Res. 2005 Jan 1-15;79(1-2):119-27 Authors: Rodrigues TB, Gray HL, Benito M, Garrido S, Sierra A, Geraldes CF, Ballesteros P, Cerdán S We report a novel ((13)C, (2)H) nuclear magnetic resonance (NMR) procedure to investigate lactate recycling through the monocarboxylate transporter of the plasma membrane of cells...
nmrlearner Journal club 0 11-24-2010 11:14 PM
[NMR paper] A novel 19F-NMR method for the investigation of the antioxidant capacity of biomolecu
A novel 19F-NMR method for the investigation of the antioxidant capacity of biomolecules and biofluids. Related Articles A novel 19F-NMR method for the investigation of the antioxidant capacity of biomolecules and biofluids. Free Radic Biol Med. 1999 Aug;27(3-4):356-63 Authors: Aime S, Calzoni S, Digilio G, Giraudo S, Fasano M, Maffeo D A new assay for the measurement of the antioxidant capacity of biomolecules by high resolution 19F-NMR spectroscopy is presented here. This method is based on the use of trifluoroacetanilidic detectors, namely...
nmrlearner Journal club 0 11-18-2010 08:31 PM
[NMR paper] Lactic acid and protein interactions: implications for the NMR visibility of lactate
Lactic acid and protein interactions: implications for the NMR visibility of lactate in biological systems. Related Articles Lactic acid and protein interactions: implications for the NMR visibility of lactate in biological systems. Biochim Biophys Acta. 1999 Jan 4;1426(1):177-84 Authors: Chatham JC, Forder JR The addition of bovine serum albumin (BSA) to a solution of lactate and alanine resulted in the disappearance of the 1H-NMR resonances from lactate but not alanine. As temperature is increased lactate becomes increasingly NMR visible and...
nmrlearner Journal club 0 11-18-2010 07:05 PM
[NMR paper] A 19F-NMR study of the equilibrium unfolding of membrane-associated D-lactate dehydro
A 19F-NMR study of the equilibrium unfolding of membrane-associated D-lactate dehydrogenase of Escherichia coli. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-acspubs.jpg Related Articles A 19F-NMR study of the equilibrium unfolding of membrane-associated D-lactate dehydrogenase of Escherichia coli. Biochemistry. 1996 Dec 24;35(51):16502-9 Authors: Sun ZY, Pratt EA, Simplaceanu V, Ho C Partially folded protein intermediates have been observed by 19F-NMR spectroscopy during the equilibrium unfolding of the...
nmrlearner Journal club 0 08-22-2010 02:20 PM
[NMR paper] A 19F-NMR study of the membrane-binding region of D-lactate dehydrogenase of Escheric
A 19F-NMR study of the membrane-binding region of D-lactate dehydrogenase of Escherichia coli. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_FREE_120x27.gif http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.pubmedcentral.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.gif Related Articles A 19F-NMR study of the membrane-binding region of D-lactate dehydrogenase of Escherichia coli. Protein Sci. 1993 Nov;2(11):1938-47 Authors: Sun ZY, Truong HT, Pratt EA, Sutherland DC,...
nmrlearner Journal club 0 08-22-2010 03:01 AM


Thread Tools Search this Thread
Search this Thread:

Advanced Search
Display Modes Rate This Thread
Rate This Thread:

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are Off
Pingbacks are Off
Refbacks are Off



BioNMR advertisements to pay for website hosting and domain registration. Nobody does it for us.



Powered by vBulletin® Version 3.7.3
Copyright ©2000 - 2024, Jelsoft Enterprises Ltd.
Copyright, BioNMR.com, 2003-2013
Search Engine Friendly URLs by vBSEO 3.6.0

All times are GMT. The time now is 02:39 AM.


Map