BioNMR
NMR aggregator & online community since 2003
BioNMR    
Learn or help to learn NMR - get free NMR books!
 

Go Back   BioNMR > NMR community > News from NMR blogs
Advanced Search



Jobs Groups Conferences Literature Pulse sequences Software forums Programs Sample preps Web resources BioNMR issues


Webservers
NMR processing:
MDD
NMR assignment:
Backbone:
Autoassign
MARS
UNIO Match
PINE
Side-chains:
UNIO ATNOS-Ascan
NOEs:
UNIO ATNOS-Candid
UNIO Candid
ASDP
Structure from NMR restraints:
Ab initio:
GeNMR
Cyana
XPLOR-NIH
ASDP
UNIO ATNOS-Candid
UNIO Candid
Fragment-based:
BMRB CS-Rosetta
Rosetta-NMR (Robetta)
Template-based:
GeNMR
I-TASSER
Refinement:
Amber
Structure from chemical shifts:
Fragment-based:
WeNMR CS-Rosetta
BMRB CS-Rosetta
Homology-based:
CS23D
Simshift
Torsion angles from chemical shifts:
Preditor
TALOS
Promega- Proline
Secondary structure from chemical shifts:
CSI (via RCI server)
TALOS
MICS caps, β-turns
d2D
PECAN
Flexibility from chemical shifts:
RCI
Interactions from chemical shifts:
HADDOCK
Chemical shifts re-referencing:
Shiftcor
UNIO Shiftinspector
LACS
CheckShift
RefDB
NMR model quality:
NOEs, other restraints:
PROSESS
PSVS
RPF scores
iCing
Chemical shifts:
PROSESS
CheShift2
Vasco
iCing
RDCs:
DC
Anisofit
Pseudocontact shifts:
Anisofit
Protein geomtery:
Resolution-by-Proxy
PROSESS
What-If
iCing
PSVS
MolProbity
SAVES2 or SAVES4
Vadar
Prosa
ProQ
MetaMQAPII
PSQS
Eval123D
STAN
Ramachandran Plot
Rampage
ERRAT
Verify_3D
Harmony
Quality Control Check
NMR spectrum prediction:
FANDAS
MestReS
V-NMR
Flexibility from structure:
Backbone S2
Methyl S2
B-factor
Molecular dynamics:
Gromacs
Amber
Antechamber
Chemical shifts prediction:
From structure:
Shiftx2
Sparta+
Camshift
CH3shift- Methyl
ArShift- Aromatic
ShiftS
Proshift
PPM
CheShift-2- Cα
From sequence:
Shifty
Camcoil
Poulsen_rc_CS
Disordered proteins:
MAXOCC
Format conversion & validation:
CCPN
From NMR-STAR 3.1
Validate NMR-STAR 3.1
NMR sample preparation:
Protein disorder:
DisMeta
Protein solubility:
camLILA
ccSOL
Camfold
camGroEL
Zyggregator
Isotope labeling:
UPLABEL
Solid-state NMR:
sedNMR


Reply
Thread Tools Search this Thread Rate Thread Display Modes
  #1  
Unread 03-24-2019, 10:41 PM
nmrlearner's Avatar
Senior Member
 
Join Date: Jan 2005
Posts: 22,317
Points: 193,617, Level: 100
Points: 193,617, Level: 100 Points: 193,617, Level: 100 Points: 193,617, Level: 100
Level up: 0%, 0 Points needed
Level up: 0% Level up: 0% Level up: 0%
Activity: 50.7%
Activity: 50.7% Activity: 50.7% Activity: 50.7%
Last Achievements
Award-Showcase
NMR Credits: 0
NMR Points: 193,617
Downloads: 0
Uploads: 0
Default Absolute 1H polarization measurement with a spin-correlated component of magnetization by hyperpolarized MAS-DNP solid-state NMR #DNPNMR

From The DNP-NMR Blog:

Absolute 1H polarization measurement with a spin-correlated component of magnetization by hyperpolarized MAS-DNP solid-state NMR #DNPNMR

To my knowledge, this is one of the few methods that allows to measure the absolute polarization of a spin ensemble. Often the on/off signal ratio is used to characterize the efficiency of the DNP - a method that does not take depolarization effects into account. The method is related to the SPY-MR method to measure absolute spin polarization in dissolution DNP experiments.






Sugishita, Tomoaki, Yoh Matsuki, and Toshimichi Fujiwara. “Absolute 1H Polarization Measurement with a Spin-Correlated Component of Magnetization by Hyperpolarized MAS-DNP Solid-State NMR.” Solid State Nuclear Magnetic Resonance 99 (July 2019): 20–26. https://doi.org/10.1016/j.ssnmr.2019.02.001.


Sensitivity of magic-angle spinning (MAS) NMR spectroscopy has been dramatically improved by the advent of high-field dynamic nuclear polarization (DNP) technique and its rapid advances over the past decades. In this course, discussions on ways to improve the DNP enhancement factor or the overall sensitivity gain have been numerous, and led to a number of methodological and instrumental breakthroughs. Beyond the sensitivity gain, however, discussions on accurate quantification of the 1H polarization amplitude achievable in a sample with DNP have been relatively rare. Here, we propose a new method for quantifying the local 1H hyperpolarization amplitude, which is applicable to un-oriented/powdered solid samples under MAS NMR conditions. The method is based on the ability to observe the high-order spin-correlated term (2IzSz) intrinsic to a hyperpolarized I-S two-spin state, separately from the lowest-order Zeeman term (Sz) in quasi-equilibrium magnetization. The quantification procedure does not require evaluation of signal amplitudes for a “microwave-off” condition and for an un-doped reference sample, and thus enables quick and accurate quantification unaffected by the effects of the paramagnetic quenching and the MAS-induced depolarization. The method is also shown to elucidate spatial polarization distribution through the 2IzSz term prepared domain selectively. As a potential application, we also demonstrate 2D DQ-SQ spectroscopy utilizing the 2IzSz term that is generated in a spatially selective manner without using I-S dipolar or J coupling. These salient features may be evolved into a way for characterizing mesoscopic molecular assemblies of medical/biological importance.



Go to The DNP-NMR Blog for more info.
Reply With Quote


Did you find this post helpful? Yes | No

Reply
Similar Threads
Thread Thread Starter Forum Replies Last Post
New NMR tools for protein structure and function: Spin tags for dynamic nuclear polarization solid state NMR #DNPNMR
From The DNP-NMR Blog: New NMR tools for protein structure and function: Spin tags for dynamic nuclear polarization solid state NMR #DNPNMR Rogawski, R. and A.E. McDermott, New NMR tools for protein structure and function: Spin tags for dynamic nuclear polarization solid state NMR. Arch. Biochem. Biophys., 2017. 628: p. 102-113. https://www.ncbi.nlm.nih.gov/pubmed/28623034
nmrlearner News from NMR blogs 0 03-19-2018 02:52 PM
High-Field Solid-State NMR with Dynamic Nuclear Polarization #DNPNMR
From The DNP-NMR Blog: High-Field Solid-State NMR with Dynamic Nuclear Polarization #DNPNMR p.p1 {margin: 0.0px 0.0px 0.0px 36.0px; text-indent: -36.0px; font: 12.0px Helvetica} Lee, D., S. Hediger, and G.D. Paëpe, High-Field Solid-State NMR with Dynamic Nuclear Polarization, in Modern Magnetic Resonance, G.A. Webb, Editor. 2017, Springer International Publishing: Cham. p. 1-17. https://doi.org/10.1007/978-3-319-28275-6_73-1
nmrlearner News from NMR blogs 0 01-08-2018 03:44 PM
Dynamic nuclear polarization for sensitivity enhancement in modern solid-state NMR #DNPNMR
From The DNP-NMR Blog: Dynamic nuclear polarization for sensitivity enhancement in modern solid-state NMR #DNPNMR p.p1 {margin: 0.0px 0.0px 0.0px 36.0px; text-indent: -36.0px; font: 12.0px Helvetica} Lilly Thankamony, A.S., et al., Dynamic nuclear polarization for sensitivity enhancement in modern solid-state NMR. Prog Nucl Magn Reson Spectrosc, 2017. 102-103(Supplement C): p. 120-195. https://www.ncbi.nlm.nih.gov/pubmed/29157490
nmrlearner News from NMR blogs 0 11-30-2017 01:10 AM
Heteronuclear Cross-Relaxation under Solid-State Dynamic Nuclear Polarization #DNPNMR
From The DNP-NMR Blog: Heteronuclear Cross-Relaxation under Solid-State Dynamic Nuclear Polarization #DNPNMR p.p1 {margin: 0.0px 0.0px 0.0px 36.0px; text-indent: -36.0px; font: 12.0px Helvetica} Daube, D., et al., Heteronuclear Cross-Relaxation under Solid-State Dynamic Nuclear Polarization. J. Am. Chem. Soc., 2016. 138(51): p. 16572-16575. http://dx.doi.org/10.1021/jacs.6b08683
nmrlearner News from NMR blogs 0 03-07-2017 08:21 AM
Theory of solid effect and cross effect dynamic nuclear polarization with half-integer high-spin metal polarizing agents in rotating solids #DNPNMR
From The DNP-NMR Blog: Theory of solid effect and cross effect dynamic nuclear polarization with half-integer high-spin metal polarizing agents in rotating solids #DNPNMR p.p1 {margin: 0.0px 0.0px 0.0px 36.0px; text-indent: -36.0px; font: 12.0px Helvetica} Corzilius, B., Theory of solid effect and cross effect dynamic nuclear polarization with half-integer high-spin metal polarizing agents in rotating solids. Phys. Chem. Chem. Phys., 2016. 18(39): p. 27190-27204. http://dx.doi.org/10.1039/C6CP04621E
nmrlearner News from NMR blogs 0 11-21-2016 11:02 PM
A triarylmethyl spin label for long-range distance measurement at physiological temperatures using T1 relaxation enhancement #DNPNMR
From The DNP-NMR Blog: A triarylmethyl spin label for long-range distance measurement at physiological temperatures using T1 relaxation enhancement #DNPNMR Yang, Z., et al., A triarylmethyl spin label for long-range distance measurement at physiological temperatures using T1 relaxation enhancement. J. Magn. Reson., 2016. 269: p. 50-54. http://dx.doi.org/10.1016/j.jmr.2016.05.006
nmrlearner News from NMR blogs 0 08-10-2016 06:25 PM
A peripheral component interconnect express-based scalable and highly integrated pulsed spectrometer for solution state dynamic nuclear polarization #DNPNMR
From The DNP-NMR Blog: A peripheral component interconnect express-based scalable and highly integrated pulsed spectrometer for solution state dynamic nuclear polarization #DNPNMR He, Y., et al., A peripheral component interconnect express-based scalable and highly integrated pulsed spectrometer for solution state dynamic nuclear polarization. Rev Sci Instrum, 2015. 86(8): p. 083101. http://www.ncbi.nlm.nih.gov/pubmed/26329168
nmrlearner News from NMR blogs 0 06-18-2016 01:11 AM
Measuring absolute spin polarization in dissolution-DNP by Spin PolarimetrY Magnetic Resonance (SPY-MR)
From The DNP-NMR Blog: Measuring absolute spin polarization in dissolution-DNP by Spin PolarimetrY Magnetic Resonance (SPY-MR) Vuichoud, B., et al., Measuring absolute spin polarization in dissolution-DNP by Spin PolarimetrY Magnetic Resonance (SPY-MR). J. Magn. Reson., 2015. 260: p. 127-135. http://www.sciencedirect.com/science/article/pii/S1090780715002074
nmrlearner News from NMR blogs 0 01-27-2016 09:09 PM


Thread Tools Search this Thread
Search this Thread:

Advanced Search
Display Modes Rate This Thread
Rate This Thread:

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are Off
Pingbacks are Off
Refbacks are Off



BioNMR advertisements to pay for website hosting and domain registration. Nobody does it for us.



Powered by vBulletin® Version 3.7.3
Copyright ©2000 - 2023, Jelsoft Enterprises Ltd.
Copyright, BioNMR.com, 2003-2013
Search Engine Friendly URLs by vBSEO 3.6.0

All times are GMT. The time now is 08:24 AM.


Map