Pierce, Joshua, Matthew J. Cuneo, Anna Jennings, Le Li, Flora Meilleur, Jinkui Zhao, and Dean A.A. Myles. “Dynamic Nuclear Polarization Enhanced Neutron Crystallography: Amplifying Hydrogen in Biological Crystals.” In Methods in Enzymology, 634:153–75. Elsevier, 2020.
Dynamic nuclear polarization (DNP) can provide a powerful means to amplify neutron diffraction from biological crystals by 10–100-fold, while simultaneously enhancing the visibility of hydrogen by an order of magnitude. Polarizing the neutron beam and aligning the proton spins in a polarized sample modulates the coherent and incoherent neutron scattering cross-sections of hydrogen, in ideal cases amplifying the coherent scattering by almost an order of magnitude and suppressing the incoherent background to zero. This chapter describes current efforts to develop and apply DNP techniques for spin polarized neutron protein crystallography, highlighting concepts, experimental design, labeling strategies and recent results, as well as considering new strategies for data collection and analysis that these techniques could enable.
Structure of Lipid Nanoparticles Containing siRNA or mRNA by Dynamic Nuclear Polarization-Enhanced NMR Spectroscopy #DNPNMR
From The DNP-NMR Blog:
Structure of Lipid Nanoparticles Containing siRNA or mRNA by Dynamic Nuclear Polarization-Enhanced NMR Spectroscopy #DNPNMR
Viger-Gravel, J., et al., Structure of Lipid Nanoparticles Containing siRNA or mRNA by Dynamic Nuclear Polarization-Enhanced NMR Spectroscopy. The Journal of Physical Chemistry B, 2018. 122(7): p. 2073-2081.
https://doi.org/10.1021/acs.jpcb.7b10795
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05-04-2018 03:33 PM
In Situ Characterization of Pharmaceutical Formulations by Dynamic Nuclear Polarization Enhanced MAS NMR #DNPNMR
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In Situ Characterization of Pharmaceutical Formulations by Dynamic Nuclear Polarization Enhanced MAS NMR #DNPNMR
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Ni, Q.Z., et al., In Situ Characterization of Pharmaceutical Formulations by Dynamic Nuclear Polarization Enhanced MAS NMR. The Journal of Physical Chemistry B, 2017. 121(34): p. 8132-8141.
http://dx.doi.org/10.1021/acs.jpcb.7b07213
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12-09-2017 07:49 AM
Dynamic Nuclear Polarization and Relaxation of H and D Atoms in Solid Mixtures of Hydrogen Isotopes #DNPNMR
From The DNP-NMR Blog:
Dynamic Nuclear Polarization and Relaxation of H and D Atoms in Solid Mixtures of Hydrogen Isotopes #DNPNMR
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Sheludiakov, S., et al., Dynamic Nuclear Polarization and Relaxation of H and D Atoms in Solid Mixtures of Hydrogen Isotopes. Journal of Low Temperature Physics, 2016: p. 1-11.
http://dx.doi.org/10.1007/s10909-016-1730-6
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02-14-2017 12:51 AM
Dynamic Nuclear Polarization Enhanced MAS NMR Spectroscopy for Structural Analysis of HIV-1 Protein Assemblies #DNPNMR
From The DNP-NMR Blog:
Dynamic Nuclear Polarization Enhanced MAS NMR Spectroscopy for Structural Analysis of HIV-1 Protein Assemblies #DNPNMR
Gupta, R., et al., Dynamic Nuclear Polarization Enhanced MAS NMR Spectroscopy for Structural Analysis of HIV-1 Protein Assemblies. J Phys Chem B, 2016. 120(2): p. 329-39.
http://www.ncbi.nlm.nih.gov/pubmed/26709853
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05-25-2016 02:33 PM
Amplifying dynamic nuclear polarization of frozen solutions by incorporating dielectric particles
From The DNP-NMR Blog:
Amplifying dynamic nuclear polarization of frozen solutions by incorporating dielectric particles
Kubicki, D.J., et al., Amplifying dynamic nuclear polarization of frozen solutions by incorporating dielectric particles. J Am Chem Soc, 2014. 136(44): p. 15711-8.
http://www.ncbi.nlm.nih.gov/pubmed/25285480
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11-10-2014 10:59 PM
[NMR paper] Dynamic nuclear polarization-enhanced 13C NMR spectroscopy of static biological solids.
Dynamic nuclear polarization-enhanced 13C NMR spectroscopy of static biological solids.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Dynamic nuclear polarization-enhanced 13C NMR spectroscopy of static biological solids.
J Magn Reson. 2013 Jun;231:5-14
Authors: Potapov A, Yau WM, Tycko R
Abstract
We explore the possibility of using dynamic nuclear polarization (DNP) to enhance signals in structural studies of biological solids by solid state NMR...
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01-11-2014 07:50 PM
Dynamic nuclear polarization-enhanced 13C NMR spectroscopy of static biological solids
From The DNP-NMR Blog:
Dynamic nuclear polarization-enhanced 13C NMR spectroscopy of static biological solids
Potapov, A., W.M. Yau, and R. Tycko, Dynamic nuclear polarization-enhanced (13)C NMR spectroscopy of static biological solids. J Magn Reson, 2013. 231(0): p. 5-14.
http://www.ncbi.nlm.nih.gov/pubmed/23562665
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06-25-2013 12:17 AM
Dynamic nuclear polarization-enhanced 13C NMR spectroscopy of static biological solids
Dynamic nuclear polarization-enhanced 13C NMR spectroscopy of static biological solids
Available online 27 February 2013
Publication year: 2013
Source:Journal of Magnetic Resonance</br>
</br>
We explore the possibility of using dynamic nuclear polarization (DNP) to enhance signals in structural studies of biological solids by solid state NMR without sample spinning. Specifically, we use 2D 13C-13C exchange spectroscopy to probe the peptide backbone torsion angles (?,?) in a series of selectively 13C-labeled 40-residue ?-amyloid (A ?1-40) samples, in both fibrillar and...
Dynamic Nuclear Polarization Enhanced Neutron Crystallography: Amplifying Hydrogen in Biological Crystals #DNPNMR
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