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Default Zooming into the Interaction of ?-synuclein with Calcium Ions through Exclusively Heteronuclear NMR Experiments.

Zooming into the Interaction of ?-synuclein with Calcium Ions through Exclusively Heteronuclear NMR Experiments.

Related Articles Zooming into the Interaction of ?-synuclein with Calcium Ions through Exclusively Heteronuclear NMR Experiments.

Angew Chem Int Ed Engl. 2020 Jul 31;:

Authors: Pontoriero L, Schiavina M, Murrali MG, Pierattelli R, Felli IC

Abstract
Many properties of intrinsically disordered proteins (IDPs) or protein regions (IDRs) are expected to be modulated by the nature of amino acid side chains as well as by local solvent exposure. Here we propose a set of exclusively heteronuclear NMR experiments to investigate these features in different experimental conditions relevant for physiological function. The proposed approach is generally applicable to many IDPs/IDRs whose assignment is available in the BMRB to investigate how their properties are modulated by different, physiologically relevant conditions. The experiments are tested on ?-synuclein. They are then used to investigate how ?-synuclein senses Ca2+ * concentration jumps associated to the transmission of nervous signals. Novel modules in the primary sequence of ?-synuclein optimized for calcium sensing in highly flexible, disordered protein segments are identified.


PMID: 32735376 [PubMed - as supplied by publisher]



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