Well-resolved and information-rich J-spectra are the foundation for chemical detection in zero-field NMR. However, even for relatively small molecules, spectra exhibit complexity, hindering the analysis. To address this problem, we investigate an example biomolecule with a complex J-coupling network-urea, a key metabolite in protein catabolism-and demonstrate ways of simplifying its zero-field spectra by modifying spin topology. This goal is achieved by controlling pH-dependent chemical exchange...
3-O-Methyl- d -glucose mutarotation and proton exchange rates assessed by 13 C, 1 H NMR and by chemical exchange saturation transfer and spin lock measurements
3-O-Methyl- d -glucose mutarotation and proton exchange rates assessed by 13 C, 1 H NMR and by chemical exchange saturation transfer and spin lock measurements
Abstract
3-O-Methyl-d-glucose (3OMG) was recently suggested as an agent to image tumors using chemical exchange saturation transfer (CEST) MRI. To characterize the properties of 3OMG in solution, the anomeric equilibrium and the mutarotation rates of 3OMG were studied by 1H and 13C NMR. This information is essential in designing the in vivo CEST experiments. At room temperature, the ratio...
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11-25-2018 06:02 AM
[NMR paper] Spin Saturation Transfer Difference NMR (SSTD NMR): A New Tool to Obtain Kinetic Parameters of Chemical Exchange Processes.
Spin Saturation Transfer Difference NMR (SSTD NMR): A New Tool to Obtain Kinetic Parameters of Chemical Exchange Processes.
Spin Saturation Transfer Difference NMR (SSTD NMR): A New Tool to Obtain Kinetic Parameters of Chemical Exchange Processes.
J Vis Exp. 2016 Nov 12;(117):
Authors: Quirós MT, Macdonald C, Angulo J, Muñoz MP
Abstract
This detailed protocol describes the new Spin Saturation Transfer Difference Nuclear Magnetic Resonance protocol (SSTD NMR), recently developed in our group to study processes of mutual-site...
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12-04-2016 02:24 AM
[NMR paper] NMR resonance splitting of urea in stretched hydrogels: proton exchange and (1)H/(2)H isotopologues.
NMR resonance splitting of urea in stretched hydrogels: proton exchange and (1)H/(2)H isotopologues.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles NMR resonance splitting of urea in stretched hydrogels: proton exchange and (1)H/(2)H isotopologues.
J Magn Reson. 2014 Oct;247:72-80
Authors: Kuchel PW, Naumann C, Chapman BE, Shishmarev D, Håkansson P, Bacskay G, Hush NS
Abstract
Urea at ~12 M in concentrated gelatin gel, that was stretched,...
Characterizing Slow Chemical Exchange in Nucleic Acids by Carbon CEST and Low Spin-Lock Field R1? NMR Spectroscopy
Characterizing Slow Chemical Exchange in Nucleic Acids by Carbon CEST and Low Spin-Lock Field R1? NMR Spectroscopy
Bo Zhao, Alexandar L. Hansen and Qi Zhang
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja409835y/aop/images/medium/ja-2013-09835y_0005.gif
Journal of the American Chemical Society
DOI: 10.1021/ja409835y
http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA
http://feeds.feedburner.com/~r/acs/jacsat/~4/iu74AOgzY6s
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12-19-2013 05:34 AM
Lecture 10. 13C NMR Chemical Shifts. Chemical Equivalence and Spin-Spin Coupling.
Lecture 10. 13C NMR Chemical Shifts. Chemical Equivalence and Spin-Spin Coupling.
http://i.ytimg.com/vi/y3IyHEVNi_Q/default.jpg
Lecture 10. 13C NMR Chemical Shifts. Chemical Equivalence and Spin-Spin Coupling.
This video is part of a 28-lecture graduate-level course titled "Organic Spectroscopy" taught at UC Irvine by Professor James S. Nowick. The course covers in...
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03-07-2013 05:38 AM
[NMR paper] Probing Slow Chemical Exchange at Carbonyl Sites in Proteins by Chemical Exchange Saturation Transfer NMR Spectroscopy.
Probing Slow Chemical Exchange at Carbonyl Sites in Proteins by Chemical Exchange Saturation Transfer NMR Spectroscopy.
Probing Slow Chemical Exchange at Carbonyl Sites in Proteins by Chemical Exchange Saturation Transfer NMR Spectroscopy.
Angew Chem Int Ed Engl. 2013 Feb 28;
Authors: Vallurupalli P, Kay LE
Abstract
Seeing the invisible: A 13 CO NMR chemical exchange saturation transfer (CEST) experiment for the study of "invisible" excited protein states with lifetimes on the order of 5-50 ms has been developed. The 13 CO chemical...
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03-02-2013 11:45 AM
[NMR paper] Membrane protein topology probed by (1)H spin diffusion from lipids using solid-state
Membrane protein topology probed by (1)H spin diffusion from lipids using solid-state NMR spectroscopy.
Related Articles Membrane protein topology probed by (1)H spin diffusion from lipids using solid-state NMR spectroscopy.
J Am Chem Soc. 2002 Feb 6;124(5):874-83
Authors: Huster D, Yao X, Hong M
We describe a two-dimensional solid-state NMR technique to investigate membrane protein topology under magic-angle spinning conditions. The experiment detects the rate of (1)H spin diffusion from the mobile lipids to the rigid protein. While spin...
Zero-Field NMR of Urea: Spin-Topology Engineering by Chemical Exchange
Linear Mode
